OL Biosafety-1 - Week 7 Labster Biosafety Lab worksheet. PDF

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Week 7 Labster Biosafety Lab worksheet....

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BIOS242, Week 7-OL Name:

Lab 7: Biosafety Learning Outcomes    

Explain how a biosafety containment level III laboratory is constructed (e.g. air flow and pressure control). Explain the basic safety rules of a biosafety containment level III laboratory (e.g. use of safety equipment). Handle microorganisms in a biosafety containment level III laboratory. Explain the concept of fumigation and how this is executed within a microbiological safety cabinet.

Introduction: Experiment with biosafety virtual simulation lab In the Biosafety simulation, you will be introduced to the concept of laboratory containment, which is a very special type of laboratory required to work with hazardous pathogens. Laboratory containment Containment in this context refers to the requirements set out by various bodies to safely manipulate such pathogens. In this simulation, you will be able to experience a containment level 3 (Biosafety Level 3 or BSL3) research laboratory. Learn about lab safety You will become familiar with the basic protocols utilized to ensure safety, and you will be presented with the specific basic safety rules for this type of laboratory (e.g. personal protective equipment (PPE) and primary containment). In addition, you will learn how every part of the laboratory is designed to avoid contamination. This includes learning about why the pressure in the laboratory needs to be controlled at all times (secondary containment), and how the microbiological safety cabinets are designed to offer protection). Identify the hazard In the biosafety simulation you will be presented with a hypothetical case where you will need to identify a potential bioterrorism agent that is classed as a hazard group three microorganism. Please note that this simulation has been developed broadly around UK regulations. Some protocols may differ depending on the specific regulations.

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BIOS242, Week 7-OL Name:

Questions: 1. Purpose: Please describe in complete sentences and in your own words, the purpose of this experiment. 

The goal of this experiment was to become familiar with the various levels of biosafety and to conduct a Bacillus anthracis test appropriately and safely. Another objective was to get knowledge about how to effectively manage a spill cleanup.

2. What are the different biosafety levels?  

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Level 1 organisms are those that are highly unlikely to cause disease. Level 2 organisms are those that can cause disease in humans and posing a threat to those who are directly exposed to them. It is improbable that this will spread to the community. Level 3 organisms are those that can cause significant sickness in humans and posing a threat to those who are directly exposed. There is a risk that it will spread throughout the community. Level 4 organisms are those that can cause major disease in humans and posing a serious threat to those who are exposed to them. There is a high danger that I will spread throughout the neighborhood.

3. What practices would you recommend being used in BSL 1 and how do these differ from BSL 2?  I would recommend specific techniques for BSL 1 since they can be employed near other facilities and do not require the use of special contamination equipment in the workplace. Gowns or lab coats, gloves, and goggles should all be used. These should be different than those in a BSL 2 since microorganisms in a BSL 2 can cause sickness; consequently, the doors should be self-closing to prevent microbes from entering or exiting. Experiments that may involve splashes or spills should be conducted inside a cabinet, and equipment should be decontaminated using a method such as autoclaving. A BSL 2 facility should be more separated, with access restricted to those authorized. There should be distinctions between the two since BSL 2 carries a risk of disease and requires

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BIOS242, Week 7-OL Name:

additional protocols and policies to prevent humans from becoming ill or the microbe from being handled properly. 4. In this simulation what organism was discussed? Which biosafety level is this pathogen handled in? Why? 

Bacillus anthracis should be treated with biosafety level 3 precautions because it has the potential to cause serious disease and pose a threat to the population. While it is unknown whether Bacillus anthracis is present, it should be treated as level 3 to be on the safe side.

5. What does biosafety level 4 mean? What type of pathogens can only be handled in these labs? 

Biosafety level 4 pathogens pose a significant threat to the community and can cause severe disease in people. Ebola and Rift Valley sickness are only a few of the pathogens dealt with at the level 4.

6. Reflection: Write 5 sentences on what you learned from this simulation. What did you like and what was something that you would prefer not to be a part of this simulation?

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What I learned from this simulation is that there are various levels of biosafety, ranging from zero risk to the highest risk of disease and spread throughout the population. I learned about Bacillus anthracis, a rod-shaped gram-positive bacterium. It is classed as a level 3 pathogen because it produces serious sickness in humans and poses a danger of spreading to the community. When working with a potentially dangerous material such as Bacillus anthracis, it is critical to wear appropriate personal protective equipment (PPE) to protect oneself and to prevent dirt and other contaminants from entering or exiting the lab. The

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BIOS242, Week 7-OL Name:

personal protective equipment (PPE) that was worn and should be worn includes a lab gown, gloves, and an overcoat.

Grading Rubric: Activity Lab Report and Questions

All Lab Deliverables

Deliverable Complete answer questions 

Purpose (2 point)

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Questions (8 points)

 Reflection (5 points) Complete ALL lab work and lab report

Points 15

15

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