Microscope Questions - Lab 3 PDF

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Introduction to the Microscope PRE-LAB QUESTIONS 1. Of the four major types of microscopes, give an example of a scenario in which each would be the ideal choice for visualizing a sample.

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Stereo (dissecting) – 100x – visible light - used for small macro organisms, too large for compound microscope – teaching and research labs.

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Compound – 2000x – visible light - inexpensive and used for microscopic cells – teaching and research labs.

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Confocal – 2000x – laser - used for visualizing individual components and create 3D images – medical and research labs.

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Electron – 10X10^6x – electron beam – needs metal coating – used for most minute structures and create 3D images – large medical and research labs.

2. What is the difference between the coarse adjustment knob and the fine adjustment knob? When is it appropriate to use each of them?

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The coarse and fine adjustment knobs are both for focusing, but the fine adjustment knob is used to focus more on precise and with greater detail. You can only use the fine adjustment knob when using a higher magnification because the slide is so close that the coarse adjustment knob could break the slide.

3. If you are using a compound microscope with an ocular lens with a magnification of 10x and an objective lens with a magnification of 40x, what is the total magnification?

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Introduction to the Microscope 

400

4. In this lab, we discussed preparation of a wet mount slide. Research and describe another slide preparation and an example of when it would be used.

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Dry mount slide – prepared like wet mount slides but without the water. Example of when it would be used for: inanimate objects or things that do not need water to live.

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Introduction to the Microscope EXPERIMENT 1: VIRTUAL MICROSCOPE Post-Lab Questions 1. What is the first step normally taken when you look through the ocular lenses?

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To adjust the eye pieces to line up into the light circle correctly.

2. What does it mean that the image is inverted when you look through the ocular lenses?

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When looking through the ocular lenses it creates an inverted image because the mirrors that directs the light that comes off the image to your eyes.

3. What new details are you able to see on the slide when the magnification is increased to 10x that you could not see at 4x? What about at 40x?

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The higher the magnification the more details you can see on the slide because it is zooming in on a certain area. When the magnification was only a 4x, you could see a lot more of the overall object and when you get to 40x it zooms in to more specific area and you can see the different thickness and layers.

4. Why is it important to be able to properly calibrate and measure objects viewed through a microscope?

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Properly calibrating the microscope is important so that you can quickly and easily get a measurement of the objects that are being examined. Every microscope is different, and the measurements can be off if not calibrate correctly.

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Introduction to the Microscope 5. Describe the qualitative difference you notice with the different types of microscope views in the “Microscope Compare” and “Specimen Compare” exercises.

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The qualitative difference I noticed right away was the colors from the confocal microscope on the microscope compare view. The confocal microscope showed very bright, electric colors, which I found appealing. I am very visual and found the colors to be more helpful and show more detail.

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