Most antibodies have KD values in the low micromolar (10-6) to nanomolar (10-7 to 10-9) range. High affinity antibodies generally considered to be in the low nanomolar range (10-9) with very high affi PDF

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Summary

抗原抗体结合的亲和力范围已经tcr与pmhc结合的亲和力
This part of the reaction is used to calculate the "on-rate" (Kon), a constant used to characterize how quickly the antibody binds to its target....

Description

mcAb（单克隆抗体）的亲和力通常为 1×105～1×1012mol-1

What is the typical KD value for an antibody? What would one expect to be a good KD value?

Most antibodies have KD values in the low micromolar (10-6) to nanomolar (10-7 to 10-9) range. High affinity antibodies generally considered to be in the low nanomolar range (10-9) with very high affinity antibodies being in the picomolar (10-12) range. Association Reaction (Kon) - This part of the reaction is used to calculate the "on-rate" (Kon), a constant used to characterize how quickly the antibody binds to its target.

Dissociation Reaction (Koff) - This part of the reaction is used to calculate the "off-rate" (Koff), a constant used to characterize how quickly an antibody dissociates from its target. TCR 对其 pMHC 亲和力很低,因此很难直接预测 TCR 与 pMHC 的结 合亲和力。 because the weak binding affinities of natural TCR-pMHC interactions (0.1–500μM) are close to the limits of detection by SPR....