PEX-08-04 - Physio Ex 9.1 PDF

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Physio Ex 9.1...

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Name: Steffany Rivera Exercise 8: Chemical and Physical Processes of Digestion: Activity 4: Assessing Lipase Digestion of Fat Lab Report Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. Which of the following is/are true of bile? You correctly answered: b. It works by a physical process. 2. The substrate used in this simulation is You correctly answered: c. vegetable oil. 3. When fatty acids are liberated by lipase, the pH You correctly answered: b. decreases. 4. One of the products of the chemical digestion of lipids is You correctly answered: d. fatty acids.

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Experiment Results Predict Question: Predict Question: Which tube do you think will have the highest lipase activity? Your answer : c. tube 3 Stop & Think Questions: What does the pH measure? You correctly answered: d. both lipase activity and fatty acid release Why is it difficult to detect whether lipase is active in tube 5? You correctly answered: d. The pH is already very low, so a decrease in pH might be difficult to detect. Experiment Data: Tube No.

Reagent 1

Reagent 2

Reagent 3

Reagent 4

1 2

Lipase Lipase

Vegetable Oil Vegetable Oil

pH 7.0 Buffer pH 7.0 Buffer

3 4 5

Lipase Deionized Water Lipase

Deionized Water Vegetable Oil Vegetable Oil

Bile Salts Deionized Water Bile Salts Bile Salts Bile Salts

6

Lipase

Vegetable Oil

Bile Salts

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Temp.

pH

60 60

37 37

6.21 7.00

pH 9.0 Buffer pH 7.0 Buffer pH 2.0 Buffer

60 60 60

37 37 37

9.00 7.00 2.00

pH 9.0 Buffer

60

37

9.00

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Post-lab Quiz Results You scored 75% by answering 3 out of 4 questions correctly. 1. What is the product of lipase hydrolysis? You correctly answered: d. fatty acids 2. From your results, which pH is ideal for pancreatic lipase digestion? You correctly answered: b. pH 7.0 3. Which tube confirms that there is no lipase in bile salts or vegetable oil? You correctly answered: c. tube 4 4. From your results, where (in theory) would pancreatic lipase be active? Your answer: c. pancreas Correct answer: d. mouth and pancreas

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Review Sheet Results 1. Explain why you can't fully test the lipase activity in tube 5. Your answer: Because there is already a low pH so it is really hard to measure the change in pH 2. Which tube had the highest lipase activity? How well did the results compare with your prediction? Discuss possible reasons why it may or may not have matched. Your answer: Test tube 1 had the highest lipase activity because of the low pH 3. Explain why pancreatic lipase would be active in both the mouth and the pancreas. Your answer: Because some fats might need to be broken down in both the mouth and the pancreas 4. Describe the process of bile emulsification of lipids and how it improves lipase activity. Your answer: Bile emulsification increases the surface area and allows for more lipase activity by allowing it to bind to the active site.

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