Physio EX-01-01 PDF

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Name: Lily Rosenblum Exercise 1: Cell Transport Mechanisms and Permeability: Activity 1: Simulating Dialysis (Simple Diffusion) Lab Report Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. The driving force for diffusion is You correctly answered: b. the kinetic energy of the molecules in motion. 2. In diffusion, molecules move You correctly answered: a. from high concentration to low concentration. 3. Which of the following dialysis membranes has the largest pore size? You correctly answered: d. 200 MWCO 4. Avogadro's number is a constant for the number of You correctly answered: b. molecules.

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Experiment Results Predict Question: Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane? Your answer : c. No, not at all. Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607 amino acids, and the average molecular weight of a single amino acid is 135 g/mole. Which of the following will be able to diffuse through the 200 MWCO membrane? Your answer : c. glucose only Stop & Think Questions: The reason sodium chloride didn't diffuse left to right is that You correctly answered: c. the membrane pore size was too small. Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average molecular weight of a single amino acid is 135 g/mole. There is no reason to run these solutes at the 20 MWCO because You correctly answered: d. glucose and albumin are both too large to pass. The rate of diffusion for urea You correctly answered: b. is slower than that for sodium because urea is a larger molecule. Experiment Data: Solute

MWCO

Left Solute Concentration

Na+ ClUrea Albumin Glucose Na+ ClUrea

20 20 20 20 20 20

9.00 0.00 0.00 0.00 0.00 9.00

0.00 0.00 0.00 0.00 0.00 0.00

0.0000 0.0000 0.0000 0.0000 0.0000 0.0000

Albumin Glucose Na+ ClUrea Albumin Glucose

20 20 50 50 50 50

0.00 0.00 9.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00

0.0000 0.0000 0.0150 0.0000 0.0000 0.0000

Na+ ClUrea Albumin Glucose Na+ ClUrea

50 50 50 50 100 100

18.00 0.00 0.00 0.00 9.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00

0.0273 0.0000 0.0000 0.0000 0.0150 0.0000

Albumin Glucose Na+ ClUrea

100 100 100 100

0.00 0.00 0.00 9.00

0.00 0.00 0.00 0.00

0.0000 0.0000 0.0000 0.0094

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Right Solute Concentration

Average Diffusion Rate

Solute

MWCO

Left Solute Concentration

Albumin Glucose

100 100

0.00 0.00

0.00 0.00

Na+ ClUrea Albumin Glucose Na+ ClUrea

200 200 200 200 200 200

0.00 0.00 0.00 9.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00

0.0000 0.0000 0.0000 0.0042 0.0000 0.0000

Albumin Glucose

200 200

9.00 0.00

0.00 0.00

0.0000 0.0000

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Right Solute Concentration

Average Diffusion Rate 0.0000 0.0000

Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. The effect of increasing the concentration of sodium chloride from 9 mM to 18 mM in the left beaker was to You correctly answered: b. increase the rate of diffusion. 2. Describe the difference between the rate of diffusion seen for sodium and urea. You correctly answered: c. Urea diffused more slowly because it is larger than sodium. 3. Which of the following solutes did not pass through any of the membranes? You correctly answered: b. albumin 4. When diffusion stops, we say the solution has reached You correctly answered: a. equilibrium.

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Review Sheet Results 1. Describe two variables that affect the rate of diffusion. Your answer: Temp and Concentration gradient. 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? Your answer: The urea wasn't able to diffuse through the 20 MWCO membrane because the molecular weight of urea is 60.07. This value is larger than the 20 MWCO. My results were accurate with my prediction. 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? Your answer: Glucose was able to slightly diffuse through the 200 MWCO membrane because it is smaller than the membrane. The molar mass of Glucose is 180.156g/mole which is less than 200 MWCO. However, the albumin was unable to because its molar mass is too large. My results are accurately reflected in my prediction 4. Put the following in order from smallest to largest molecular weight: glucose, sodium chloride, albumin, and urea. Your answer: Sodium Chlorie: 58.44 g/mol Urea: 60.06 g/mol Glucose: 180.156 g/mol Albumin: 66000 g/mol

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