Physio Ex Exercise 8 Activity 2 PDF

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3/23/2021

PhysioEx Exercise 8 Activity 2

PhysioEx Lab Report Exercise 8: Chemical and Physical Processes of Digestion Activity 2: Exploring Amylase Substrate Specificity Name: Titus Aina Date: 23 March 2021 Session ID: session-42c7d3de-7cd8-bb96-1723-f65851f24670

Pre-lab Quiz Results You have not completed the Pre-lab Quiz.

Experiment Results Predict Question 1 Predict Question: Do you think test tube 3 (amylase, cellulose, pH 7.0 buffer) will show a positive Benedict's test? Your answer: yes. Stop & Think Questions 1 You included tube 2 (amylase, glucose, pH 7.0 buffer) to You correctly answered: see what a positive Benedict's test should look like. 2 Which tube is included to detect contaminating amylase in the buffer or in cellulose? You correctly answered: None of the above. 3 What is the usual substrate for the pancreatic enzyme peptidase? You correctly answered: peptides. Experiment Data Tube No. Reagent 1 Reagent 2 Reagent 3

Time (min)

Temp (°C)

IKI

Benedict's

1

Amylase

Starch

pH 7.0 Buffer

60

37

-

++

2

Amylase

Glucose

pH 7.0 Buffer

60

37

-

++

3

Amylase

Cellulose

pH 7.0 Buffer

60

37

-

-

4

Cellulose

pH 7.0 Buffer

Deionized Water

60

37

-

-

5

Peptidase

Starch

60

37

+

pH 7.0

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3/23/2021

PhysioEx Exercise 8 Activity 2

5

Peptidase

Starch

Buffer

6

Bacteria

Cellulose

pH 7.0 Buffer

60

37

+

-

60

37

-

++

Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 The substrate(s) for amylase is/are You correctly answered: starch. 2 The results of tube 5 (peptidase, starch, pH 7.0 buffer) demonstrated that You correctly answered: peptidase does not digest starch. 3 Why was the cellulose in tube 6 (bacteria, cellulose, pH 7.0 buffer) hydrolyzed to glucose? You correctly answered: The bacterial suspension contained the enzyme cellulase, which digested the cellulose. 4 Which tube allows you to conclude that the buffer was not contaminated with amylase? You correctly answered: tube 5 (peptidase, starch, pH 7.0 buffer).

Review Sheet Results 1 Describe why the results in tube 1 (amylase, starch, pH 7.0 buffer) and tube 2 (amylase, glucose, pH 7.0 buffer) are the same. Your answer: The results showed that tube 1 and tube 2 were the same because the amylase in tube 1 is hydrolyzing the starch to the glucose. In tube 2, the glucose is already in its hydrolyzed form. 2 Describe the result in tube 3 (amylase, cellulose, pH 7.0 buffer). How well did the results compare with your prediction? Your answer: I originally predicted yes, however after experimenting, I found that test tube 3 did not show Benedict's test. This is because the amylase did not digest the cellulose in the solution. 3 Describe the usual substrate for peptidase. Your answer: The usual substrate for peptidase is peptides and proteins. https://media.pearsoncmg.com/bc/bc 0media ap/physioex/10/ex8/act2/

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PhysioEx Exercise 8 Activity 2

4 Explain how bacteria can aid in digestion. Your answer: Bacteria aids in digestion by breaking down cellulose; this is because the body cannot do it naturally without cellulase.

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