Physio Ex Exercise 8 Activity 4 PDF

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11/9/2020

PhysioEx Exercise 8 Activity 4

PhysioEx Lab Report Exercise 8: Chemical and Physical Processes of Digestion Activity 4: Assessing Lipase Digestion of Fat Name: Kaelyn Wozniak Date: 9 November 2020 Session ID: session-45b6ac50-6de1-cd2e-b150-f7eae7f80ff9

Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 Which of the following is/are true of bile? You correctly answered: It works by a physical process. 2 The substrate used in this simulation is You correctly answered: vegetable oil. 3 When fatty acids are liberated by lipase, the pH You correctly answered: decreases. 4 One of the products of the chemical digestion of lipids is You correctly answered: fatty acids.

Experiment Results Predict Question 1 Predict Question: Which tube do you think will have the highest lipase activity? Your answer: tube 3 (lipase, deionized water, bile salts, pH 9.0 buffer). Stop & Think Questions 1 What does the pH measure? You correctly answered: both lipase activity and fatty acid release. 2 Why is it difficult to detect whether lipase is active in tube5(lipase, vegetable oil, bile salts, pH 2.0 buffer)? You correctly answered: The pH is already very low, so a decrease in pH might be difficult to detect. Experiment Data https://media.pearsoncmg.com/bc/bc 0media ap/physioex/10/ex8/act4/

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11/9/2020

PhysioEx Exercise 8 Activity 4

Tube No

Reagent 1

1

Lipase

2 3 4

Reagent 2 Vegetable Oil

Reagent 3

Reagent 4

Time (min)

Temp (°C)

pH

Bile salts

pH 7.0 Buffer

60

37

6.21

Lipase

Vegetable Deionized Oil Water

pH 7.0 Buffer

60

37

7.00

Lipase

Deionized Water

Bile salts

pH 9.0 Buffer

60

37

9.00

Deionized Vegetable Water Oil

Bile salts

pH 7.0 Buffer

60

37

7.00

5

Lipase

Deionized Water

Bile salts

pH 2.0 Buffer

60

37

2.00

6

Lipase

Vegetable Oil

Bile salts

pH 9.0 Buffer

60

37

9.00

Post-lab Quiz Results You scored 75% by answering 3 out of 4 questions correctly. 1 What is the product of lipase hydrolysis? You correctly answered: fatty acids. 2 From your results, which pH is ideal for pancreatic lipase digestion? You correctly answered: pH 7.0. 3 Which tube confirms that there is no lipase in bile salts or vegetable oil? You correctly answered: tube 4 (deionized water, vegetable oil, bile salts, pH 7.0 buffer). 4 Where in the body does pancreatic lipase hydrolyze triglycerides? Your answer: mouth. Correct answer: small intestine.

Review Sheet Results 1 Explain why you can't fully test the lipase activity in tube5(lipase, vegetable oil, bile salts, pH 2.0 buffer). Your answer: The lipase in tube 5 can not be fully tested because the pH is already so low and measuring the lipase activity would drop the pH even more. i https://media.pearsoncmg.com/bc/bc 0media ap/physioex/10/ex8/act4/

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11/9/2020

PhysioEx Exercise 8 Activity 4

2 Which tube had the highest lipase activity? How well did the results compare with your prediction? Discuss possible reasons why it may or may not have matched. Your answer: Test tube 1 ended up having the highest lipase activity and this is because it was the closest pH to the intestines. 3 Explain why pancreatic lipase would be active in both the mouth and the pancreas. Your answer: Pancreatic lipase would be active in both the mouth and the pancreas because it has the highest activity in areas with a pH of 7.0 which is what the mouth and pancreas are. 4 Describe the process of bile emulsification of lipids and how it improves lipase activity. Your answer: The process of bile emulsification of lipids occurs due to bile physically breaking up the fat globules. It improves lipase activity because of the breaking down physically and not chemically.

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