Robert Bednarczyk Micro Lec Lab 1 Spring 2019 BS4420-001 Syllabus PDF

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SUNY College at Old Westbury Biological Sciences Spring 2019 BS 4420-001, Microbiology Lecture: Tuesdays and Thursdays 8:00-9:30AM; NSB S103 Laboratory Section: Tuesdays 9:40AM-12:50PM; NSB S102

Lecture Instructor: Robert Bednarczyk, PhD Email: [email protected] To help me respond to your emails more quickly, please include “MICRO-Old Westbury” in the subject line. I will attempt to answer your e-mails within 24 hours or less. Office Phone: 516-876-3041 Office: NSB S-205 Office Hours: Mondays 1:00-3:00pm; Tuesdays and Thursdays 1:00-4:00pm; Wednesdays 10:00-12:00pm; Other times are only by scheduled appointments Prerequisites: Prior to taking this course, you should have taken an introductory biology course, e.g. BS2400/BS2401 and BS2410/BS2411. Course Information: This course is an introduction to the fundamentals of microbiology, intended to serve science majors. The course is divided in three units: (I) Introduction to Microbiology, (II) Microbial Nutrition, Growth, and Control (III) Microbial Molecular Biology and Genetics and (IV) Diversity of the Microbial World. Refer to the calendar of the course to see the specific topics we will cover in the course. Laboratory sessions are focused on basic techniques and procedures used by microbiologist such as pure culture techniques, methods of staining and the microscopic, colonial and biochemical identification of microorganisms. Course Objectives: After completing this course you should have an understanding of: 1. The importance of microorganisms to our global society (students are invited to bring relevant news articles to class for discussion). 2. The basic groups of microbes, including prokaryotic microbes (Archaea and Bacteria), viruses, and eukaryotic microbes. 3. The structure and function of cells, their metabolism and how they grow/divide. 4. The eukaryotic and prokaryotic genomes, and gene expression in each group.

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5. The tools and techniques that are used to classify and identify microbes as well as those used in biotechnology to develop products. 6. The principles of chemotherapy and discusses the ideal characteristics for successful chemotherapeutic agents. Required Material:  Textbook: Prescott’s Microbiology, 10th Edition. McGraw Hill, Publishers. ISBN 978-1-25928159-4. The textbook will be used as a resource for the lecture. Pages of the textbook that correlate with the corresponding lecture topics are listed on the Lecture schedule. Reading the textbook may help you understand and be able to apply concepts presented in class but, unless specifically noted in class, you will not be tested on topics that are not discussed in the lecture or lab, or included in handouts or supplements on the course website. There will be one available hard-copy textbook on Course Reserve at the Library. 

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Laboratory Manual: Laboratory Exercises in Microbiology; Prescott, Harley, Klein. McGraw Hill publishers. There will be one available hard-copy manual on Course Reserve at the Library. A mobile device enabled for WiFi access and an account in Socrative (https://www.socrative.com/apps.html)

Class Resources: Blackboard: We will use the Blackboard platform for this course. To get access to the course use the following Course ID: _____________________________. Lecture handouts, videos, animations, additional readings, and study guides will be posted on Blackboard on a weekly basis. Your classmate: Identifying at least two study partners will serve you well. These classmates should be your go-to people when you have a question after class, if you come late, or miss a class. Write their names and email or phone number. Consider forming study groups. Name: __________________________________________ Phone: _________________________________________ Email: __________________________________________

Name: __________________________________________ Phone: _________________________________________ Email: __________________________________________

Name: __________________________________________ Phone: _________________________________________ Email: __________________________________________

Name: __________________________________________ Phone: _________________________________________ Email: __________________________________________

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Preparing for Class: Reading and Assignments: Lecture will focus on review and reinforcement of assigned material; therefore assignments should be completed prior to each class time. Each assignment will have a set of questions to guide your reading and reinforce what you have independently learned from the reading. Complete the assignment before the next class lecture topic. You should bring to class: 1. The lecture handouts posted on Blackboard (either printed or on laptop/tablet) to assist in note taking. 2. Extra blank paper for drawings, notes, and activities during class. 3. A mobile device to use the “Socrative” app. Style of exams: The questions on the exam will be: multiple choice, true or false, short answer, essay, and/or matching. The question will vary from simple recall to interpretative/analytical types where you will demonstrate a deeper understanding of a concept. What to bring and not bring to the exam: 1. A pencil or a pen, whatever you prefer. 2. Calculators, cellphones, smart watches or any other electronic devices are not to be used. 3. You will need your Old Westbury ID card when turning in the exam. Class Courtesy:  Only coffee, tea, juice, water or any other beverage is accepted in the classroom . Do not bring food or beverage into the laboratory.  Class interruptions by taking or making calls, texting, or using phones for social media are prohibited. If you are seen making one of this you will be ask to leave the classroom.  Inappropriate clothing in classroom is not permitted (e.g. pajamas, provocative clothes, Halloween costume, etc.); if you ware inappropriate cloth you will be ask to leave the room.  No visitors are allowed in the classroom or laboratory. Attendance Policy:  Lecture Attendance: Lectures provides essential information not covered in the text. It is therefore important that you attend all lectures. If you miss a lecture you are responsible for the material covered. You are also responsible for all announcements made in lecture. If you miss a class or come late, be sure you obtain missed material from a classmate.  Laboratory Attendance: Attendance at every laboratory section is required. If you must miss a laboratory you must provide documentation to the laboratory instructor that the absence was unavoidable. Failure to attend more than 2 of your assigned laboratory automatically results in an F for the course.

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Lecture Exams:  There will be four exams. Three unit exams and a final exam will be given on the dates listed on the syllabus. The exam will assess your ability to retain and recall material covered in lecture as well as your ability to integrate and extrapolate the material covered by solving problems. o o o o

Exam 1: February 19, 2019; 8:00-9:30am Exam 2: March 12, 2019; 8:00-9:30am Exam 3: April 16, 2019; 8:00-9:30am Final Exam: Finals week May 14-16, 2019

NOTE: This syllabus serves as a guide for lectures, homework and exams. The lecturer may make changes as the semester progresses. If changes are made, an announcement will be posted on Blackboard. 

Make-up exams: If, for a valid reason, (illness, etc.) you are unable to take an exam at the scheduled time, you must contact me within 24 hours. The make-up exam will be in a different format than the one given to the class. If you fail to notify me, you will receive a “zero” (0) for the grade. The policy for missing the final is the same.

Grading Policy: Your grade in this course will be determined by lecture and laboratory as follows:  Lecture Grade (70% of the final grade):  Partial exams: Scores from the 3 exams will be combined for a total of 55% of your lecture grade.  Final exam: will account for 20% of your lecture grade.  Blackboard quizzes: There will be one quiz per chapter. Your two lowest quiz score will be dropped. Scores from the quizzes will be combined for a total of 10% of your lecture grade.  Class Participation: Your class participation will be based on your attendance, class participation and punctuality. Participation will account for a 5% of your lecture grade.  Group presentation and written report: You will be presenting in small groups of 5 on a ‘directed' topic of your choice. To ensure that every student participate in the process each member will write a report. ‘Presentation Guide and Instructions' will be provided in Blackboard. This will account for a 10% of your lecture grade. 

Laboratory Grade (30% of the final grade): o Attendance: will account for 25% of your laboratory grade.

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o Lab participation and completion of laboratory assessments in your Lab Manual: will account for 25% of your laboratory grade. o Laboratory exams: 50% of your laboratory grade will come from the two laboratory exams. Grading Scale:  Please note that there is no curving at the end of the semester. Point Total 97 – 100 93 – 96 90-92 87-89 83-86 80-82 77-79 73-76 70-72 60-69 0-59

Grade A+ A AB+ B BC+ C CD F

There are no negotiations for grades. Your grade is the sum of the components listed above. There is no extra credit, make-up points. It is expected that you give 100% effort in all your activities in this course.

Disability Resources: In order to receive disability-related academic accommodations, students must first be registered with the Office of Services for Students with Disabilities (OSSD). Students who have a documented disability or suspect they may have a disability are invited to set up an appointment with the director of the OSSD. The office will help you determine if you qualify for accommodations and assist you with the process of accessing them. All support services are free and all contacts with the OSSD are strictly confidential. If you have already registered with the OSSD, please provide the course accommodation form and discuss your specific accommodations with me. All information and documentation is confidential. SUNY/Old Westbury is committed to assuring that all students have equal access to all learning activities and to social activities on campus. Office for Students with Disabilities is in the Academic building, Room 2065 Director: Stacey DeFelice Phone: 516-876-3009 Email: [email protected]

Honor Code:

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Each student must pursue his or her academic goals honestly and be personally accountable for all submitted work. Representing another person’s work as your own is always wrong. Faculty is required to report any suspected instances of academic dishonesty. For more comprehensive information on academic integrity, including categories of academic dishonesty, please refer to https://www.oldwestbury.edu/policies/academic-integrity. The following represents examples of this but does not constitute an exhaustive list:  Cheating on exams or assignments by the use of books, electronic devices, notes, or other aids when these are not permitted including collusion or copying from another student.  Submitting the same paper in more than one course or repeatedly within the same course without permission of the instructors.  Plagiarizing: copying someone else’s writing or paraphrasing it too closely, even if it constitutes only some of your written assignment. Falsifying documents or records related to credit, grades, or other academic matters.  Altering an exam or paper after it has been graded in order to request a grade change.  Stealing, concealing, destroying, or inappropriately modifying classroom or other instructional material, such as posted exams, laboratory supplies, or computer programs.  Preventing relevant material from being subjected to academic evaluation. “Please note: In this matter, ignorance of the Academic Integrity Policy is never an acceptable excuse”

Week 1 2

Date Jan 22 Jan 24 Jan 29

Topic

Reading

Introduction: Evolution/Classification/History

Chapter 1

Study of Microbial Structure

Chapter 2

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3 4 5 6 7 8 (Mid-term) 9 10 11 12

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14 15 16 17 (Finals)

Jan 31 Feb 5 Feb 7 Feb 12 Feb 14 Feb 19 Feb 21 Feb 26 Feb 28 Mar 5 Mar 7 Mar 12 Mar 14 Mar 19 Mar 21 Mar 26 Mar 28 Apr 2 Apr 4 Apr 9 Apr 11 Apr 16 Apr 18 Apr 23 Apr 25 Apr 30 May 2 May 7 May 9 May 14 May 16

Microscopy and Specimen Preparation Microbial Taxonomy

Chapter 19

Prokaryotic Cell Structure and Function

Chapters 3, 4

Eukaryotic Cell Structure and Function ***EXAM I*** (Chapter 1, 2, 3, 4 & 19) Eukaryotic Cell Structure and Function Acellular Infectious Agents Microbial Growth Control of Microorganisms in the Environment Antimicrobial Chemotherapy ***EXAM II*** (Chapter 5-9) Bacterial Genome Replication and Expression

Chapter 5 Chapter 5 Chapter 6 Chapter 7 Chapter 8 Chapter 9 Chapter 13

Spring Break-no classes Regulation of Bacterial Cellular Processes

Chapter 14

Genetic Variations Recombinant DNA Technology and Microbial Genomics Viruses: Introduction and General Characteristics; Bacteriophages; Viruses of Eukaryotes ***EXAM III*** (Chapter 13, 14, 16, 17, 18) Viruses: Introduction and General Characteristics; Bacteriophages; Viruses of Eukaryotes

Chapter 16

Protista, Fungi, Algae

Chapters 25, 26

Chapters 17, 18 Chapter 27

Chapter 27

Students Presentation Review Study day ***FINAL*** (All)

SUNY College at Old Westbury Biological Sciences Spring 2018 BS 4420, Microbiology Laboratory Instructor: Robert Bednarczyk, PhD

Section: Tuesday 9:40AM-12:50PM

Email: [email protected] Office Phone: 516-876-3041

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Office: NSB S-205 Office Hours: Mondays 1:00-3:00pm; Tuesdays and Thursdays 1:00-4:00pm; Wednesdays 10:00-12:00pm; Other times are only by scheduled appointments Laboratory Manual: Laboratory Exercises in Microbiology; Prescott, Harley, Klein. McGraw Hill publishers. DATES Jan 29 Feb 5

Feb 12

Feb 19 Feb 26 Mar 5 Mar 12 Mar 26, Apr 2 Apr 9, Apr 16 Apr 23 Apr 30

LAB Harley #1 Harley #2 Harley #14 Harley #15 Harley #16 Harley #17 Harley #19 Harley #6 Harley #7 Harley #8 Harley #9 Harley #45

Harley #18 Harley #20 Harley #38 Harley #36 Harley #62

TOPIC General Directions and Safety Requirements Bright-Field Light Microscopy-Bacterial Motility Culture Transfer Instruments & Techniques Spread – Plate Technique Streak – Plate Technique Pour – Plate Technique Determination of Bacterial Numbers Negative Staining Smear – Preparation and Simple Staining Gram Staining Acid Fast Determination of a Bacterial Growth Curve, Two Hour Method ***EXAM*** Cultivation of Anaerobe Bacteria: Fermentation Unknowns Entrotube Bacterial Transformation

Hand Out

Transduction

Harley #61

Bioinformatics ***FINAL***

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