SIM - Lab Assignment about SIM (Sulfur Reduction, Indole Production, and Motility) PDF

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Lab Assignment about SIM (Sulfur Reduction, Indole Production, and Motility)...

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Ali Gilblair SIM Medium (Indole)

Sim medium is a combination differential medium that tests three different parameters, Sulfur Reduction, Indole Production, and Motility. The sulfur reduction test is useful in differentiating enteric organisms. The Indole test is a component of the IMViC series of tests which are used for differentiating the Enterobacteriaceae. The motility test is useful for testing a wide variety of organisms. The SIM test is primarily useful for differentiating Salmonella and Shigella. SIM mediums contain nutrients, iron, and sodium thiosulfate. If an organism can reduce to hydrogen sulfide, the hydrogen sulfide will combine with the iron to form ferric sulfide, which is also a black precipitate. If there is any blackening of the medium, that indicates the reduction of sulfur which is a positive test result. For Hydrogen Sulfide, a positive test will show blackening along the line of inoculation and a negative test will show no blackening of the medium. A positive result for the Indole test will show red color development in the upper portion of the medium and a negative will show no red color. For the Motility test, a positive result will show diffuse growth outward from the stab line or turbidity through the medium, and a negative result will show growth only along the line of inoculation....