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Ali Gilblair Snyder Test

The Snyder test is a colorimetric test for determining dental caries, or cavities, activity or susceptibility based on the rate of acid production by acidogenic oral microorganisms (lactobacillus) in a glucose medium. Bromcresol green is used as the pH indicator, and it produces a color change from green to yellow. The Snyder test agar includes 2% glucose. The pH of the agar is ~ 4.8, which inhibits the growth of most organisms, but it is ideal for acidophiles such as Lactobacillus species. Saliva samples are inoculated into the tubes and allowed to incubate. If Lactobacillus is present in the saliva, it will ferment the glucose and produce lactic acid, causing the pH to drop to ~ 4.4, causing the bromcresol to change from a green color to a yellow. A change of color to yellow indicated the individual from which the sample came from is susceptible to the formation of dental caries (cavities). No color change indicates little or no susceptibility to forming dental caries, a slight color change indicates mild susceptibility to forming dental caries, significant color change indicates moderate susceptibility to forming dental caries, and complete color change indicates high susceptibility to forming dental caries....