Subject Outline Spring 2021 - Immunology 1 PDF

Preview

Summary

Subject Outline Spring 2021 for Immunology 1...

Description

SUBJECT OUTLINE 91401 Immunology 1 Course area

UTS: Science

Delivery

Spring 2021; City

Credit points 6cp Requisite(s)

91400c Human Anatomy and Physiology These requisites may not apply to students in certain courses. See access conditions.

Result type

Grade and marks

Attendance: Spring session: 11wks (2hpw, lecture; 3hrs, practical session [every alternating week]; 2x 2hrs, workshops, online). February session: 3wks (4 x 3hrs, practical sessions [3x face-to-face, 1x online]; 2x 2hrs, workshops, online; 10 x 2hrs, lectures)

Subject coordinator Dr Richard Kim Lecturer & Postdoctoral Research Fellow Centre for Inflammation, School of Life Sciences, Faculty of Science Tel: 9565 6198 Email: [email protected] Room: CB04.06.440 (meetings by appointment only)

Teaching staff Lecturers Dr Richard Kim, Lecturer Email: [email protected] Dr Chantal Donovan, Lecturer Email: [email protected] Dr Lisa Sedger, Lecturer Email: [email protected] Dr Gang Liu, Lecturer Email: [email protected] Dr Matt Johansen, Post-doctoral Researcher Email: [email protected] Prof. Phil Hansbro, Lecturer Email: [email protected] Queries regarding lecture content should be directed to the responsible Lecturer or the Subject Coordinator. Queries involving practicals, ask your Teaching Associates (TAs) during practical sessions otherwise email your Lead Teaching Associate or the Subject Coordinator.

Subject description This subject is designed to introduce the basic concepts of immunology for students planning careers in biomedical research, biotechnology and diagnostic pathology. The cells of the blood, bone marrow and immune tissues are studied in detail with regard to their identification, morphology and function. The development of these cells (haematopoiesis) and their role in haemostasis and immune function is investigated. The study of immune function is structured in such a way that it follows the course of an immune response, from initial non-specific reactions to the development of adaptive responses and immunological memory. Emphasis is given to the basic concepts that underlie the recognition of foreign bodies by the host and the immune response to infection. The practical sessions introduce students to a variety of immunological techniques routinely used in pathology and research laboratories such as serial dilutions, Coombs test, enzyme-linked immunosorbent assay (ELISA) and flow cytometry.

23/07/2021 (Spring 2021)

© University of Technology Sydney

Page 1 of 9

Subject learning objectives (SLOs) Upon successful completion of this subject students should be able to: 1. Work safely in a laboratory using blood, cell products and perform tests following safety guidelines and using laboratory safety precautions. 2. Identify, describe and relate innate immune cell morphology and function. Specifically identify monocyte, macrophage, NK cell, granulocytes and their effector mechanisms for clearing foreign bodies including phagocytosis, free radicals of oxygen and oxygen-independent killing, perforin and granzyme mediated killing. 3. Describe, demonstrate and analyse the complement cascades (alternative, classical and lectin pathways) and their role in opsonisation, cell lysis and the inflammatory response. 4. Identify, describe and relate adaptive immune cell morphology and function. Specifically describe B and T cells and their unique receptors which bind a unique antigen and induce the proliferation/differentiation of effector cell clones (clonal selection). 5. Describe and relate immature, mature, plasma and memory B cells. Specifically describe the structural organisation of the antibody molecule and how this facilitates antibody function. 6. Describe and relate the role of the thymus in the development of mature T cells. 7. Describe and justify the structure and function of the T cell receptor. 8. Describe and explain the two T cell MHC-restricted (class I and class II) antigen processing and presentation pathways.

Course intended learning outcomes (CILOs) This subject also contributes specifically to the development of following course intended learning outcomes: Apply: Identify how disease can arise and disrupt normal physiological function. (1.1) Analyse: Examine and use appropriate scientific tools in the design and execution of medical science research. (2.2) Synthesise: Work responsibly, safely, and with respect to diversity, within ethical, academic, and regulatory frameworks relevant to medical science. (3.3)

Contribution to the development of graduate attributes Immunology 1 develops the desired Faculty of Science graduate attributes of: 1. Disciplinary knowledge An understanding of the nature, practice and application of the fundamental concepts underlying the discipline of immunology. The knowledge and understanding of immunological concepts and techniques studied and practiced in this subject will aid in developing competence in relevant, current laboratory skills for professional practice and research. In this subject, you will also acquire the sound theoretical background to make reasoned scientific and technological judgments and make responsible decisions affecting laboratory operations. These concepts and techniques will be achieved through the subject’s theoretical curriculum and through the provision of practical experience in the practical sessions. 2. Research, inquiry and critical thinking You will engage in practical and workshop learning experiences that present case studies and experimental data, which will develop your skills in data analyses, problem-solving and critical thinking. 3. Professional, ethical and social responsibility Through the practical and workshop program, you will develop the ability to acquire, develop, employ and integrate a range of technical, practical and professional skills, within a professional context, both autonomously and collaboratively, across the disciplinary area of immunology. During practical sessions, there will be opportunities to develop your expertise in laboratory skills and data handling and analysis.

23/07/2021 (Spring 2021)

© University of Technology Sydney

Page 2 of 9

Teaching and learning strategies Each week during the teaching session you will participate in lectures (theory) (2 hour duration each) and practicals/workshops (2-3 hour duration each) including wet-lab experiments that will be performed in student groups. For further information regarding the content involved, please refer to the subject 'program' in the following section. Lectures, practicals, and workshops are closely integrated to help you achieve this subject’s learning objectives. Before attending each scheduled lecture, you must download the slides/additional information from CANVAS and prepare before the class. For Immunology 1 theory revision, you will be provided with lecture revision modules (quiz questions) from week 2 on CANVAS in preparation for the Immunology 1 Theory exam conducted towards the end of the teaching session. Practical classes will be conducted in laboratories and involve hands-on approaches to learning. You will have an Immunology 1 Practical Manual and experienced Teaching Associates to assist, guide and offer feedback to you through the practical component of this subject. Before coming to each scheduled practical class, you must revise the material provided in the Immunology 1 Practical Manual as this will allow you to maximise your learning experience in the face-to-face sessions. There will be learning activities where you will work in groups and share findings with other students. Immunology 1 Practical session knowledge will be assessed through online Practical Reports (short answer and multiple choice questions). Your Immunology 1 Practical Manual, Lecture Slides/Notes, lecture revision modules, and other additional resources will be made available to you through CANVAS, as will results and feedback, other than the final Theory exam results. It is essential that CANVAS is checked at least twice weekly to receive updated material and announcements.

Content (topics) The lecture series is designed to introduce you to the language (jargon) and basic concepts of immunology. The practical sessions provide an introduction to a variety of techniques and procedures that are the cornerstones of immunological analysis in clinical and research laboratories. In addition, workshop sessions and problem-based learning activities will be used to explore contemporary topics in immunology and to provide opportunities to apply knowledge to real life situations.

Program Week/Session

Dates

1

Description 2nd August 2021 Lecture 1. Introduction to the Immune System: Cells and Organs Notes: Two hour lectures are delivered online on Mondays (6pm-8pm). Three hour practical sessions are delivered on Tuesdays (10am-1pm, 2-5pm, 6-9pm). Bring lab coats, lab safety glasses and wear suitable closed footwear to all practical classes. If you do not wear suitable closed footwear to practical classes, you will be asked to leave the class. Please visit CANVAS for the subject welcome and subject resources. 02/08/2021 to 06/08/2021 - Preview week, No Practical.

2

9th August 2021 Lecture 2. Innate Immunity 1

23/07/2021 (Spring 2021)

© University of Technology Sydney

Page 3 of 9

3

16th August 2021 Lecture 3. Innate Immunity 2 17th August 2021 Practical 1. Subject Overview, Laboratory Safety Orientation Serial Dilutions & Titres Doubling & logarithmic dilutions Determination of the titre of a haemolytic substance Notes: Bring lab coats, lab safety glasses and wear suitable closed footwear to all practical classes.

4

23rd August 2021 Lecture 4. Antigen Presentation

5

30th August 2021 Lecture 5. B cells 1 31st August 2021 Practical 2. Complement-mediated lysis of red blood cells (classical complement pathway) Notes: Bring lab coats, lab safety glasses and wear suitable closed footwear to all practical classes.

6

6th September 2021 Lecture 6. B cells 2

7

13th September 2021 Lecture 7. T cells 14th September 2021 Practical 3. Enzyme linked immunosorbent assay (ELISA) Completion of ELISA protocol and analysis of data. Notes: Bring lab coats, lab safety glasses and wear suitable closed footwear to all practical classes.

23/07/2021 (Spring 2021)

© University of Technology Sydney

Page 4 of 9

STUVAC

STUVAC (20th September 2021-26th September 2021) 20th September 2021. No Lecture 21st September 2021. No Practical

8

27th September 2021 Lecture 8. T cell Receptor

9

4th October 2021 No lecture 5th October 2021 Workshop 1. Flow Cytometry Workshop, Practical considerations and applications, Analysis & interpretation of data.

10

11th October 2021 Lecture 9. Regulation of T cell responses 16th October 2021 Workshop 2. Vaccines Workshop.

11

18th October 2021 Lecture 10. Immunology Research 19th October 2021 Practical 4. Flow Cytometry Practical, Analysis & interpretation of data.

12

25th October 2021 - No Lecture Online Lecture Review Modules

STUVAC

STUVAC (1st November 2021 - 5th November 2021)

Centrally conducted exams begin 6th November 2021 Date TBA Immunology 1 Theory exam Notes: Restricted open book (authorised exam resource) Immunology 1 Theory exam, 2 hour MCQ and short answer exam.

23/07/2021 (Spring 2021)

© University of Technology Sydney

Page 5 of 9

Assessment The theory and practical content delivered in Immunology 1 will be assessed with the following weighting (theory and practical session content 100%).

Assessment task 1: Quizzes Intent:

This assessment task contributes to the development of the following graduate attributes: 1. Disciplinary Knowledge.

Objective(s): This assessment task addresses subject learning objective(s): 2, 4 and 5 This assessment task contributes to the development of course intended learning outcome(s): 1.1 Type:

Quiz/test

Groupwork:

Individual

Weight:

45%

Task:

These are multiple choice and short answer question quizzes that assess knowledge, research, inquiry and critical thinking of content covered in the Immunology 1 Lectures.

Due:

Week 5, Week 9 and Week 11

Criteria:

You will be assessed on your disciplinary knowledge and interpretative skills developed in the Immunology 1 lectures. Correctness of responses to short answer and multiple-choice answer questions related to the Immunology 1 lectures.

Assessment task 2: Practical Reports Intent:

This assessment task contributes to the development of the following graduate attributes: 1. Disciplinary Knowledge. 2. Research, inquiry and critical thinking. 3. Professional, ethical and social responsibility.

Objective(s): This assessment task addresses subject learning objective(s): 1, 3 and 8 This assessment task contributes to the development of course intended learning outcome(s): 1.1, 2.2 and 3.3 Type:

Report

Groupwork:

Individual

Weight:

20%

23/07/2021 (Spring 2021)

© University of Technology Sydney

Page 6 of 9

Task:

These are Practical Reports comprised of multiple choice and short answer questions that assess knowledge, research, inquiry and critical thinking of content covered in the Immunology 1 Practical Sessions.

Due:

Week 7 and Week 12

Criteria:

Correctness of responses to multiple-choice and short answer questions related to the practical work. Correctness of interpretations and conclusions of sample experimental data from techniques studied in the Immunology 1 practical sessions

Assessment task 3: Theory Exam Intent:

This assessment task contributes to the development of the following graduate attributes: 1. Disciplinary Knowledge. 2. Research, inquiry and critical thinking.

Objective(s): This assessment task addresses subject learning objective(s): 2, 3, 4, 5, 6, 7 and 8 This assessment task contributes to the development of course intended learning outcome(s): 1.1 and 2.2 Type:

Examination

Groupwork: Individual Weight:

35%

Task:

This is a multiple choice question and short answer question examination. 2 hours (plus 10 minutes reading time) will take place during the formal examination period. This is a restricted open book exam.

Length:

2 hours

Due:

UTS Exam period Spring semester

Criteria:

You will be assessed on your disciplinary knowledge and interpretative skills developed in the Immunology 1 lectures, practical sessions and workshops. Correctness of responses to short answer and multiple-choice answer questions related to the Immunology 1 lectures and practical sessions and workshops.

Further You should note that academics do not organise these exam times, this is done centrally within the information: university. Therefore, if you cannot attend the exam or if you believe that you are entitled to Special Consideration for the exam then you need to lodge the relevant Professional Authority and online Special Consideration Application through Student Administration Services. If you miss the formal theory exam, then you will be required to sit an alternative Theory exam and the UTS Examinations Branch will inform you of the alternate date and location.

23/07/2021 (Spring 2021)

© University of Technology Sydney

Page 7 of 9

Minimum requirements You must attain at least 50% of the collective marks in the subject to pass. No minimum attendance requirement for practicals. No minimum requirements for lectures.

Recommended texts Prescribed readings for Immunology 1 will be from the text below, which is also the recommended text for Immunology 2 (subject number 91359) Murphy K and Weaver C (9th Edition) Janeway's Immunobiology Garland Science NY (ISBN 978-0-8153-4505-3)

Academic liaison officer Academic liaison officers Download the complete list of the University's ALOs (PDF 52kB), including their contact details.

Support UTS Library The Library has a wide range of resources, facilities and services to support you throughout your studies including textbooks, subject readings, old exam papers, academic writing guides, scientific literature databases, workshops, a gaming room and bookable group study rooms. There is also a team of librarians to help you with all your questions. w: lib.uts.edu.au facebook: utslibrary twitter: @utslibrary ph: 9514 3666 Mathematics & Science Study Centre The Mathematics and Science Study Centre (MSSC) operates a Drop-in Room located on UTS City Campus, in Building 4, level 3, room 331 (CB04.03.331). Academic staff members are available for one-to-one assistance. For timetabling and other MSSC resources see: w:https://tinyurl.com/UTS-maths-study-centre

Statement on copyright Australian copyright law allows you as a student or researcher to copy and use limited amounts of other people's material in your study or research without their permission and free of charge. This applies to any sort of published or unpublished work, and includes written material, tables and compilations, designs, drawings (including maps and plans), paintings, photographs, sculpture, craft work, films (such as feature films, television programs, commercials and computer video games), software (such as computer programs and databases), sound recordings, performances and broadcasts (including podcasts and vodcasts of these) and text, including books, journals, websites, emails and other electronic messages. It is important to remember that you can only use a limited amount for your study or research purposes and that you need to correctly acknowledge the author and reference their material when you use it in your work. Incorrect or improper use of copyright-protected material could result in breaking Australian copyright law, for which significant penalties apply. Incorrect or improper use of copyright-protected material at UTS would result in consideration under the UTS Student Misconduct rules. UTS Rules and the UTS Student Charter require that students familiarise themselves and comply with UTS student policies and procedures. Student should also see the copyright information advising what you can copy and how much you can use. Copyright notice concerning teaching materials Please remember that the teaching materials and resources provided to you at UTS are protected by copyright. You are not permitted to re-use those for commercial purposes (including in-kind benefit or gain) without permission of the copyright owner. Improper or illegal use of teaching materials may lead to prosecution for copyright infringement. Your presentation submitted as an Assessment may be used for future teaching activities at UTS without further notification.

23/07/2021 (Spring 2021)

© University of Technology Sydney

Page 8 of 9

Statement on plagiarism The University and Faculty of Science encourage students to undertake their academic studies with the highest integrity and take seriously any instances of student misconduct. Student misconduct as defined by Rule 16.2 can include cheating (examples of which may be in formal or informal examinations, copying work from another student for individual reports or assignments, altering data,...