TLC Plan - Lab summary/plan PDF

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Lab summary/plan...

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Victoria Puckett, Anthony Mba, Walker Lierman TLC Plan 04/26/2018 Planning Questions: 1. We will transfer the ink from the letter to the TLC plate by soaking it in ethanol and using the solution on the TLC plate. 2. We will use the most polar and the least polar to determine which one is better for TLC separation. After completing a few trials and determining which are best, then we will mix those and test the separation to see if there is more separation in the process. 3. We will determine which ink matches the unknown by testing a couple solvents and figuring out which ones had the most seperation. Some things to consider is that the ink is not as strong as it should.

Procedure: 1. Gather Materials and prepare for experiment’ 2. Obtain 4 different solvents 3. Place mobile phase into beaker as well as TLC plate. 4. Seal beaker 5. Wait for indicated end point 6. Take out TLC plate and mark results with pencil so the results don’t fade 7. Calculate RF 8. Record DATA

Calculations:

Distance traveled by spot

Rf= distnace traveled by solvent front

Glassware/Specific Techniques - Thin-layer chromatography - Solution preparation - Determination of Rf - Glass Dish

MSDS/SDS ●

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Ethanol ( C❑2 H ❑6 O ) ○ CAS#: 64-17-5 ○ Acute Health Effects: ■ Hazardous in case of skin contact (irritant), of eye contact (irritant), of inhalation. Slightly hazardous in case of skin contact (permeator), of ingest ○ Chronic Health Effects: ■ Slightly hazardous in case of skin contact (sensitizer). The substance is toxic to blood, the reproductive system, liver, upper respiratory tract, skin, central nervous system ○ ○ ○

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If in contact with eyes remove any contact lenses, flush eyes with water for 15 minutes If in contact with skin flush skin with water for 15 minutes while removing clothing. Cover skin with an anti-bacterial cream If ingested do not force oneself to vomit unless directed, if large quantities are ingested call a doctor immediately

Ethanol Acetate ( C❑4 H ❑8 O ❑2 ) ○ ○

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CAS#: 141-78-6 Acute Health Effects: ■ Hazardous in case of ingestion, of inhalation. Slightly hazardous in case of skin contact (irritant, permeator), of eye contact (irritant). Chronic Health Effects: ■ The substance is toxic to mucous membranes, upper respiratory tract. The substance may be toxic to blood, kidneys, liver, central nervous system (CNS). Repeated or prolonged exposure to the substance can produce target organs damage. If in contact with eyes remove any contact lenses, flush eyes with water for 15 minutes If in contact with skin flush skin with water for 15 minutes while removing clothing. Cover skin with emollient If ingested do not force oneself to vomit unless directed, if large quantities are ingested call a doctor immediately...