Transpiration and Stomatal Peel Worksheet PDF

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Grand Canyon University BIO-182L Transpiration and Stomatal Peel Worksheet ...

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Name:

Sydni Frazier

Transpiration and Stomatal Peel Worksheet Directions: Complete the tables, calculations, and questions below. Be sure the observations are recorded accurately. Two or three students may work together; however each student must provide answers in his or her own words. Thoroughly answer the questions below in complete sentences using collegiate level writing style. Any use of outside resources should have in-text citations and be included at the end of the assignment in GCU style. Transpiration Data X

Y

Table 1 Transpirational Data Straw Description (color) 3 blue lines 3 green lines 4 white lines 3 orange lines 3 white lines

Inside Diameter (mm) 2.2 1.5 1.0 0.7 0.5

Total Water Line Capillary Tube (mm) 15 15 17 22 26

Water Height in Petri Dish (mm) 12 12 12 12 12

Height Traveled in Capillary Tube (mm) 3 3 5 10 14

Include a copy of the Excel graph generated for this data table in the box below. Be sure to include the regression line equation and R2 on the graph.

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Name:

Sydni Frazier

Transpiration Questions Directions: Thoroughly answer the questions below in complete sentences. Any use of outside resources should have in-text citations and be listed at the end of the assignment in GCU style, and original wording is expected for all answers. Failure to follow these directions can be considered plagiarism or cheating that can result in point deductions and referral for Code of Conduct violations. 1.

Explain how transpirational pull works. Include the two main forces involved. Water molecules adhere to each other by forcing a column in the xylem through cohesion. The negative pressure of the columns produced by transpirational pull produces a force on the particles of water which causes their upward movement in xylem. Two main forces involved are cohesion, water molecules sticking to each other and adhesive force between water molecules, hydrogen bonding between water and cellulose of the xylem cell wall (Urry, Cain, Wasserman, Minorsky, & Campbell, 2017, p. 792).

2.

Why does the size of the capillary tube influence the height that the water is able to reach? How does this translate to plants and water transport? The measure of water in a tube is inversely proportional to the size of the cylinder, which implies the more slender the tube the higher the water will rise. This translates to water transportation in plants because plants move water through their xylem, which is similar to capillary tubes (Urry, Cain, Wasserman, Minorsky, & Campbell, 2017, p. 792-793).

Stomatal Peel Data Upper Epidermis Observation:

Lower Epidermis Observation :

(Note total magnification) 100x

(Note total magnification) 100x

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Table 2 Number of Stomata

Field of View (same magnification)

# Stomata Upper Epidermis

# Stomata Lower Epidermis

1

10

10

2

2

19

3

3

17

Average

5

15.3

Calculations 1. Average number of stomata/microscopic field a. Upper epidermis 10+2+3=15 15/3=5 b.

Lower epidermis 10+19+17=46 46/3 = 15.3

2. Area of observation (πr2) = ______0.16________mm2 3. Number of stomata per 1 mm2 a. Upper epidermis 5/0.16= 31.25 mm2 b.

Lower epidermis 15/0.16= 95.63 mm2

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Stomatal Peel Questions

Directions: Thoroughly answer the questions below in complete sentences. Any use of outside resources should have in-text citations and be listed at the end of the assignment in GCU style, and original wording is expected for all answers. Failure to follow these directions can be considered plagiarism or cheating that can result in point deductions and referral for Code of Conduct violations. 1.

Which leaf surface had more stomata per mm2, the upper or lower epidermis? The lower epidermis leaf surface had more stomata per mm2.

2.

What advantage does this arrangement provide for the plant? Be specific. The advantage this arrangement provides for the plant is less water loss when the plant is taking in CO2. The lower epidermis is not as exposed to the sun compared to the upper epidermis (Adam and Bledsoe, 2016).

3.

Explain how the plant uses stomata to regulate transpiration and water loss. Be specific. The plant uses stomata to regulate transpiration and water loss by photosynthesis. Stomata allow for the uptake of carbon dioxide while expelling water as it evaporates along with, expelling oxygen into the outside environment (Adam and Bledsoe, 2016).

4.

What adaptations would you expect to see in the leaf of a desert plant vs. the leaf of a tropical plant? Why? What advantages or disadvantages does each have? Most desert plants don't have leaves, yet rather have a smooth green surface with thistles which are exceptionally adjusted leaves adjusted to decrease water loss, yet also fall off during dry periods. Tropical plants have green fully developed leaves with a waxy covering in order to drip off the excess water but are often unstable due to the wet and loose soil (Dimmitt, 2020).

References: Adam, N., & Bledsoe, S. (2016). Transpiration and Stomatal Peel Lab. Retrieved from https://lc.gcumedia.com/bio182l/laboratorymanual-for-general-biology-ii/v2.1/#/chapter/5 Dimmitt, M. (2020). How Plants Cope with the Desert Climate. Retrieved from https://www.desertmuseum.org/members/sonorensis/week1.php Urry, L. A., Cain, M. L., Wasserman, S. A., Minorsky, P. V., & Campbell, N. A. (2017). Campbell biology(11th ed.). Retrieved from https://viewer.gcu.edu/g9BWpE

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