Tutorial 1 Quiz Practice Questions PDF

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Room to answer the question (which are in the lab book). Just print out the document. Questions are only for Quiz 1. ...

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1. Nucleotides, Nucleic Acids & the Properties of DNA & RNA 1. St r uct ur eofPur i ne&pyr i mi di nenucl eot i desandt hei rphosphor yl at edder i vat i v es ;pol y mer i c nucl eot i des :DNA,t her eposi t or yofgenet i ci nf or mat i onandRNA,t hemedi at orofi t s ex pr es si on 2. Thet hr eemaj orcl as sesofc el l ul arRNA–mes sengerRNA,r i bosomalRNAandt r ans f erRNA; hy br i di sat i onofDNAandRNA;RNAsecondar yst r uc t ur e;out l i neoft her ol eofRNAi npr ot ei n s ynt hes i s . 3. Pol ynucl eot i des–nat ur eandpr oper t i esoft hephosphodi es t erl i nk age;s i ngl eanddoubl es t r anded DNAs t r uct ur e;nucl eas es :enzy meswhi c hdegr adepol y nucl eot i des. 4. Pr oper t i esofDNA–si z er angeofnat ur al l yocc ur r i ngDNAmol ecul es;effec toft emper at ur e,DNA hy br i di sat i on,shearf or ces ,ac i d,al k al i ;t hec ondens at i onofl ongDNAmol ecul esi nv i v o( v i a f or mat i onofnucl eosomes ,30nm fibr es,s ol enoi dset c )i nt oc hr omat i ns t r uct ur e. 5. Pr oper t i esandusesofr es t r i ct i onenzy mes 6. Thebas i cpr i nci pl esofmol ec ul arcl oni ng

a) Whati st her epeat i nguni tofDNA?

b) Whi char et hepur i nebases?Whi char et hepyr i mi di nebases?

c) Whi chbasesar edi ffer entbet weenDNAandRNA?

d) Whati st hepoi ntofat t achmentofapur i newi t hasugari nanucl eot i de ?

e) Whati st hepoi ntofat t achmentofapyr i mi di newi t hasugari nanucl eot i de ?

f ) Whati saphosphodi est erbond?Whati st heaci dt hatr eact st of or m aphosphodi est erbond?

g) Whati smeantby‘ pol ar i t y’ ?

h) Whati smeantbyt het er m‘ ant i par al l el ?

i ) Whatar et hef or ceswhi chst abi l i set heDNAdoubl ehel i x?

j ) DNAandRNAar ebot hhi ghl ynegat i vel ychar ged.Whi chgr oupscar r yt hi schar ge?

k) Wr i t eoutashor t handr epr esent at i onf or :adeoxynucl eosi de,a5’ nucl eot i de,a5nucl eosi de t r i phosphat e,a5’ deoxynucl eosi det r i phosphat e,adi nucl eot i dewi t ha5’ hydr oxylanda3’ phosphat e.

l ) Compar et heeffectofal kal ionDNAandRNAandwhyi si tdi ffer ent ?

m)Definet het er msendonucl easeandexonucl ease.

n) Whi chchemi calgr oupsar er esponsi bl ef ort hest r ongabsor pt i onofUVl i ghtbynucl ei caci ds?

o) Whatdot het er ms‘ GCcont ent ’and‘ Tm’meanasappl i edt oDNA?

p) How doesTm var ywi t hGCcont ent ?

q) Whatar et hemai ncomponent sofchr omat i n?

r ) Whati sanucl eosome ?

s) “ I thasnotescapedourat t ent i ont hatt hespeci ficpai r i ngwehavepost ul at edi mmedi at el y suggest sapossi bl ecopyi ngmechani sm f ort hegenet i cmat er i al ”–Wat son&Cr i ck( 1953) . Whatwast hepai r i ngandhow doesi tsuggestar epl i cat i onmechani sm?

t ) Whatar et het womaj orst epsi ngeneexpr essi on?

u) Whatar et het hr eemai ncl assesofRNA?

v) Whatt ypeofenzymear er est r i ct i onenzymes?

w)Wr i t edownsomepossi bl eDNAsequencest hatmayber ecogni sedbyar est r i ct i onenzyme/

x) Pr oduct sofr est r i ct i onenzymedi gest i onofDNAcanhavecohesi veORbl untends.Whatdoes t hi smean?

y) Assumi ngar andom ar r angementofbasesi nDNA,whatar et heaver agel engt hsoff r agment s pr oduct sbyr est r i ct i onenz ymeswi t h:( a)f ourbaser ecogni t i onsequenceand( b)6base r ecogni t i onsequence?

z ) Descr i bet hepr ocessofcl oni ngaDNAf r agmentusi ngar est r i ct i onenzyme....