Type of column chromatography PDF

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column chromatography is one of the important topic in chemistry and they are used to separate protein based on their unique qualities i tried to show different type of columns through pictures and described each separately...

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Type of column chromatography a.) Stationary Phase in column chromatography and Mobile phase in column chromatography. similarities --> they are used to retain the solvents, either polar or nonpolar. differences --> the stationary phase does not moves, and typically is polar (silica) for the mobile phase, we require a nonpolar solvent , it acutally moves across the TLC plate b.) Gravity Column chromatography and Flash Column chromatography gravity column --> requires solvent which move with coheisve forces

flash column --> uses vaolatile flashing properties (evaporation) of the solvents c.) Thin Layer chromatography and column chromatography. both applies the concept of stationary phase in column style difference --> thin layer chromatography uses veyr thin plates, whereas the column chromatography uses a physcal colunn as stationary phase

What concept or process allows for separation in column chromatography What are the various layers in column chromatography and explain their purpose Why were the solvents introduced into the column in a particular order? he principle of separation of substances by column chromatography involves differential adsorption of substances by adsorbent. There are two layers present in column chromatography one is the solid layer or stationary phase made of silica and the other is the liquid or mobile phase which is the solvent that we add for separating the compounds. The purpose of the stationary phase is to adsorb all the compounds of the mixture but with different binding constant depending on their polarity. The function of the mobile phase is to release the adsorbed compound from the stationary phase one at a time thus separating them. The solvents are added at a particular order because our main aim is to separate the mixture of compounds based on their difference in polarity. The more polar compounds interact strongly with the silica and hence are bound strongly compared to the non-polar one . So a less polar solvent is added to the column to first elute the nonpolar compound followed by addition of polar solvent to elute the polar compound. If the polar solvent is added first then it will elute both the polar and nonpolar compounds so we cannot separate them , hence the solvents are added in reverse order....