Virtual Microscope PDF

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Virtual microscope exercise....

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Exercise One- Virtual Microscope PRE-LAB QUESTIONS 1. Of the four major types of microscopes, give an example of a scenario in which each would be the ideal choice for visualizing a sample. - If you wanted to look at the surface of a coin much closer, a stereo microscope would be a good choice for this. - If you were comparing sand or dirt from different locations, you could use a compound microscope for this. - Confocal microscopes are used in medical and research labs. They could be used to look at vascular systems and take measurements of cross sectional areas. - Electron microscopes are used often in biomedical research. Perhaps in biomedical engineering of cells. 2. What is the difference between the coarse adjustment knob and the fine adjustment knob? When is it appropriate to use each of them? - The coarse adjustment knob gets the specimen into focus, and the fine adjustment knob helps to keep the specimen in focus when moving to larger magnifications. The fine adjustment helps create a sharper more precise image. 3. If you are using a compound microscope with an ocular lens with a magnification of 10x and an objective lens with a magnification of 40x, what is the total magnification? - 400x 4. In this lab, we discussed preparation of a wet mount slide. Research and describe another slide preparation and an example of when it would be used. - A dry mount slide could be used to examine a hair follicle after you’ve plucked it from your head. You would place your hair onto the slide and a cover slip on top of it to hold it in place before placing it under the microscope.

Post-Lab Questions 1. What is the first step normally taken when you look through the ocular lenses? - Adjusting your light and objective. There should be one circle, not two. 2. What does it mean that the image is inverted when you look through the ocular lenses? - It means if you move the slide away from you, through the lens it will appear to move toward you. 3. What new details are you able to see on the slide when the magnification is increased to 10x that you could not see at 4x? What about at 40x? - The shape of the cell along with its cell wall or cell membrane are visible at 10x, 4. Why is it important to be able to properly calibrate and measure objects viewed through a microscope? - Calibration is important to ensure accurate measuring of things too small to measure any other way. The lens alone can’t give a proper reference of an object's true size under a microscope. 5. Describe the qualitative difference you notice with the different types of microscope views in the “Microscope Compare” and “Specimen Compare” exercises. - Scanning electron microscope makes many more fine details of the specimen visible. The confocal microscope gives fluorescent images that show individual structures of the specimen. While the transmission microscope gives a much higher magnification than the Normasky optics that just give a decent general view of the sample....