Worksheet 5 PDF

Title	Worksheet 5
Author	ley ren
Course	Molecular Genetics
Institution	La Trobe University
Pages	2
File Size	55.9 KB
File Type	PDF
Total Downloads	54
Total Views	157

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worksheet 5...

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GEN3MGG Applied Genomics worksheet Instructions Fill out the following worksheet while working through the Applied Genomics lessons on LMS. You must write all answers in your own words (paraphrase). Do not plagiarise from the text or videos provided! Bring your completed worksheet to the workshop and work on your answers with your group and with the class. Submit the completed worksheet via the turnitin link by Friday, May 17 th, 11.59 pm. Note that these study questions are in the same format as for the final exam. (1 minute = 1mark).

1. Describe the four basic steps performed in Next Generation DNA Sequencing (NGS). What are the strengths and weaknesses of this approach compared with the more traditional Sanger method of DNA sequencing? (10 minutes). 1. Sample Preparation – Adaptors are added to the ends of the DNA fragments 2. Cluster Generation – Amplification of each fragment of DNA 3. Sequencing – Sequencing of DNA fragments 4. Data Analysis – Analysing DNA sequences

Strengths: 1. NGS is more sensitive than the Sanger method 2. NGS is cheaper than the Sanger method 3. NGS can sequence many genes at once unlike the Sanger method

Weaknesses: 1. NGS has a higher chance of error than the Sanger method 2. NGS is more time consuming than the Sanger method

2. What are the goals of the International Cancer Genome Consortium? What methodologies are they applying? How will their discoveries be translated into personalized medicines? (12 minutes) The ICGC aims to conduct a large-scale study in genomes using 50 different cancer types and subtypes to discover and uncover oncogenic mutations and mutagenic influences. Ultimately, the ICGC aims to enable the development of new cancer therapies.

3. Describe the steps required to carry out a genome wide mutational analysis to discover the genetic basis of cancer and outline three bioinformatics approaches that can be used to differentiate between “passenger” and “driver” mutations in the cancer genome. (10 minutes)...