Bac Culture Methods-HW PDF

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Bacteriological Culture Methods Observations and Outcomes-Fill in your answers here (10 points) Broth subcultures (Use Broth Culture Image) 2 pts Looka tt hebr ot hs ubc ul t ur et ube s ,a ndde s c r i bewha tyoue xpe c t e dt os e e ,a ndhow t he ya ppe a r i nt e r msofhow “ c l oudy”t he yl ook—c l oudi ne s si sa ni ndi c a t i onofba c t e r i a lgr o wt h. anc eof Actual appe ar anc eof Cl oudi ne s sofbr ot hbe f or e Predicted appear br ot haf t eri nc ubat i on br ot haf t eri nc ubat i on i nc ubat i on e a r l uut et uus Msub. c l r e( “S”) Cl

Yellowish

Yellowish, translucent

St e r i l i t yCont r ol( “ C” )

Yellowish

Yellowish, transparent

Cl e a r

Streak plate subcultures Looka tt hes t r e a kpl a t es ubc ul t ur e st ha ty ouma de .Conduc tas e l f a s s e s s me ntofhow we l lyou pe r -f or me dt het e c hni que .Wha tyouhopet os e ea r ei ndi vi dua lc ol oni e s ,we l ls e pa r a t e df r om e a c hot he r .Ont hes t r e a kpl a t eoft hemi x e dc ul t ur e ,y ous houl dbea bl et os e et wodi s t i nc t l y di ffe r e ntt ype sofc ol oni e s . ( 3poi nt s ) M. Lut eusSPi ma ge ) M. luteus s t r e a kpl a t e: ( •Ar et hec ol oni e swe l ls e pa r a t e d?0 . 5 pt s Thec ol oni e sar ewe l ls e pa r a t e d •How ma nydi ffe r e ntt ype sofc ol oni e sdoyous e e ?0 . 5 pt s Is e et hr e edi ffe r e ntc ol oni e s •De s c r i bei nf ul lt hec ol oni a lmor phol ogyoft heba c t e r i aont hi spl a t e .I nc l udet e xt ur e , t r a ns pa r e nc y , pi gme nt a t i on, s i ze , f or m, ma r gi nsa nde l e va t i on.1pt Thet e xt ur es e e mst obes moot ha ndgl i s t e ni ngi ns omepa r t soft hes t r e akpl a t e . Thet r a ns pa r e nc y l ooksopa quea ndve r ys ol i da ppe a r i ng. I t spi gme nti sye l l ow andi ti sme di um i ns i z ea f t e r s pr e a di ngt heba c t e r i ai nt hepl a t e . I thasafla tandr a i s e de l e va t i oni ns omepl a c e sa ndt hemar gi n s e e mst obeundul a t e . St r e a kpl a t eoft hemi x e dc ul t ur e: ( Mi x e dSPi ma ge) •Ar et hec ol oni e swe l ls e pa r a t e d?De s c r i bet hedi s t r i but i on. 1pt Mos toft hedi s t r i but i oni si nones i deoft hes t r e a kpl a t e , howe ve r , t he yar ewe l ls e pa r a t e d.

•De s c r i bei nf ul lt hec ol oni a lmor phol ogyofbot hoft heba c t e r i af r om t hemi x e dc ul t ur e : 2 pt s Col onyType1

Col onyType2

This is tough in a picture. Both are the same size

Si ze

This is tough in a picture. Both are the same size

Te xt ur e

Also tough, but both are smooth and Also tough, but both are smooth and uniform. uniform.

Tr ans par e nc y Pi gme nt a t i on Whol eCol ony

Both are opaque and not transparent

Both are opaque and not transparent

Cream Color

Cream Color

Circular

Circular

TSA slant subculture (E.coli Slant image) Ex ami net hes ubc ul t ur et ha twa spr e pa r e dont heTS As l a nt . •De s c r i bet het ex t ur e ,t r a ns par e nc y ,a ndpi gme nt a t i onoft heba c t e r i a lgr o wt hont he s l ant .Onl yt he s ec har a c t er i s t i c sc anbedes c r i be df oras l antc ul t ur e ,s i nc et her es houl d benodi s c r e e tc ol oni e sont hes l a nt , onl ya na r e aofde ns egr owt ha l ongt hes t r e ak l i ne . 1pt Thes l a nt ’ st e xt ur el ooksve r ys moot ha ndmuc oi d. I tl ookst r a ns pa r e nta tt hebe gi nni ngoft he s l a nt , buta si tc ome sdownt ot hee ndoft het e s tt ubei tl ooksopa que . I t spi gme nt a t i onl ooks ye l l o wa tt hebot t om oft het ubeandnoc ol ora tt heve r yt op. •Doe syourde s c r i pt i onma t c hwha twa snot e df or M. t het hec ol oni a lmor phol ogypr e vi ous l y?1pt l ut e us No , t hec ol oni a lmor phol ogyi sdi ffe r e ntf r om t heM. l ut e us •I st hi sapur ec ul t ur e ?1 pt Ye s , t heTSAs l a nts ub c ul t ur ei sapur ec ul t ur e .

c ol oni e swhe nyou de s c r i be d

Ada pt e df r om:Mi c r obi ol ogy:A Labor a t or yExpe r i e nc ebyHol l y Ahe r n.

Mi c r o b i o l o gy : ALa b o r a t o r yEx p e r i e nc eb yHo l l yAhe r ni sl i c e ns e dund e raCr e a t i v eCo mmo ns At t r i b ut i o nNo nCo mme r c i a l Sh a r e Al i k e4 . 0I nt e r na t i o na lLi c e ns e ,e x c e p twh e r eo t he r wi s eno t e d . ©2 0 1 8Ho l l yAhe r n. S o mer i ght s r e s e r v e d. I S BN: 9 7 8 1 9 4 2 3 4 1 5 4 3e bo o k 9 7 8 1 9 4 2 3 4 1 5 3 6pr i nt Yo ua r ef r e e t o : S ha r e —c o pya ndr e di s t r i b ut et hema t e r i a l i na n yme di um o rf o r ma t Ada pt —r e mi x , t r a ns f o r m, a ndb ui l dupo nt hema t e r i a l Thel i c e ns orc annotr e voket he s ef r e e domsa sl ongasy ouf ol l o wt hel i c e ns et e r ms . Unde rt hef o l l o wi n gt e r ms : At t r i b ut i o n—Yo umus tg i v ea ppr o pr i a t ec r e di t , pr o v i deal i nkt ot hel i c e ns e , a ndi ndi c a t ei fc ha ng e swe r ema de . Yo uma ydos oi na n yr e a s o na b l ema nne r , b utno ti na n ywa yt ha ts ugg e s t st hel i c e ns o re ndo r s e sy o uo ry our us e . No nCo mme r c i a l —Y o uma yno tus et hema t e r i a l f orc o mme r c i a l pur po s e s . S ha r e Al i k e —I fy o ur e mi x , t r a ns f o r m, o rb ui l dupo nt hema t e r i a l , y o umus tdi s t r i b ut ey o urc o nt r i b ut i o nsunde r t hes a mel i c e ns ea st heo r i gi na l . Th i spub l i c a t i o nwa sma d ep o s s i b l eb yaSUNYI nno v a t i v eI ns t r uc t i o nT e c h no l o g yGr a nt( I I TG) .I I TGi sac o mp e t i t i v egr a nt spr o gr a m o pe nt oSUNYf a c ul t ya nds up p o r ts t a ffa c r o s sa l ldi s c i p l i ne s . I I TGe nc o ur a g e sd e v e l o p me nto fi nno v a t i o nst h a tme e tt h ePo we ro f SUNY’ st r a ns f o r ma t i v ev i s i o n. Publ i s he db yOpe nS UNYT e x t bo o k s , Mi l ne Li br a r yS t a t eUni v e r s i t yo fNe w Yo r ka tGe ne s e o , Ge ne s e o , NY1 4 4 5 4...