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BIO246 MICROBIOLOGY 2020 Unit Information

BIO246 Microbiology

Unit Information Guide Semester 1, 2020

This information should be read in conjunction with the Guide to Studying at Murdoch booklet, the Unit Handbook Page at http://www.murdoch.edu.au/index/units and the material provided on the website which can be accessed via LMS

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BIO246 MICROBIOLOGY 2020 Unit Information

© Published by Murdoch University, Perth, Western Australia, 2020 This publication is copyright. Except as permitted by the Copyright Act no part of it may be in any form or by any electronic, mechanical, photocopying, recording or any other means be reproduced, stored in a retrieval system or be broadcast or transmitted without the prior written permission of the publisher.

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BIO246 MICROBIOLOGY 2020 Unit Information

Contents Unit Overview

3

Unit Objectives

4

Unit Coordination

6

Unit Structure and Timetable

7

Assessment

10

Resources

13

Unit Overview

The unit provides an introduction to the biology of viruses, fungi and bacteria, particularly the latter. One of the main emphases of the unit will be the interactions between micro-organisms and other living organisms. Topics: microbial morphology, taxonomy, culture and growth; microbes as saprophytes; soil microbiology; microbes as autotrophs; microbes as symbionts; microbes as heterotrophs; microbes as parasites (including human diseases), public health and epidemiology; applied microbiology (industrial microbiology, biological control, food production, antibiotics, genetic manipulation).

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BIO246 MICROBIOLOGY 2020 Unit Information

Unit Objectives THINKING SKILLS A student successfully completing Microbiology I will demonstrate an increased skill level in 1. Cognitive processes, including – – – –

Formulating a clear, answerable question Developing a testable hypothesis Predicting expected results Following an experimental protocol

2. Analysis skills, including – Collecting and organizing data in a systematic fashion – Presenting data in an appropriate form (graphs, tables, figures, or descriptive paragraphs) – Assessing the validity of the data (including integrity and significance) – Drawing appropriate conclusions based on the results 3. Communication skills, including – Discussing and presenting lab results or findings in the laboratory 4. Interpersonal skills, including – Working effectively in teams or groups so that the task, results, and analysis are shared – Effectively managing time and tasks allowing concurrent and/or overlapping tasks to be done simultaneously, by individuals and within a group – Integrating knowledge and making informed judgments about microbiology in everyday life

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BIO246 MICROBIOLOGY 2020 Unit Information

LABORATORY SKILLS

A student successfully completing Microbiology I should be able to demonstrate how to 1. Use a bright field light microscope to view and interpret slides, including – – – –

Correctly setting up and focusing the microscope Proper handling, cleaning, and storage of the microscope Correct use of all lenses Recording microscopic observations

2. Properly prepare slides for microbiological examination, including – – – –

Cleaning and disposing of slides Preparing smears from solid and liquid cultures Performing wet mount and/or hanging drop preparations Performing Gram stains and spore stains

3. Properly use aseptic techniques for the transfer and handling of microorganisms and instruments, including – Sterilizing and maintaining sterility of transfer instruments – Performing aseptic transfer – Obtaining microbial samples 4. Use appropriate microbiological media and test systems, including – – – –

Isolating colonies and/or plaques Maintaining pure cultures Using biochemical test media Accurately recording macroscopic observations

5. Estimate the number of microbes in a sample using serial dilution techniques, including – – – –

Correctly choosing and using pipettes and pipetting devices Correctly spreading diluted samples for counting Estimating appropriate dilutions Extrapolating plate counts to obtain the correct CFU or PFU in the starting sample

6. Use standard microbiology laboratory equipment correctly, including – Using the standard metric system for weights, lengths, diameters, and volumes – Lighting and adjusting a laboratory burner – Using an incubator

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BIO246 MICROBIOLOGY 2020 Unit Information

Unit Coordination

Unit Coordination

Graham O’Hara is the coordinator for Microbiology. All questions regarding unit administration and the laboratory component should be directed to Graham.

Contact Details for Academic Staff Graham O’Hara Rm 3.011, Biological Sciences (Building 240) Phone: 9360 2583 [email protected] Wayne Reeve Rm 3.031, Biological Sciences Phone: 9360 2631 [email protected] Jason Terpolilli Rm 3.033, Biological Sciences Phone: 9360 6104 [email protected] Ralf Cord-Ruwisch Rm 2.047, Science and Computing Phone: 9360 2403 [email protected] Giles Hardy Rm 3.037, Biological Sciences Phone: 9360 6272 [email protected] Carolyn Jones [email protected]

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BIO246 MICROBIOLOGY 2020 Unit Information

Unit Structure and Timetable Microbiology has seven hours of scheduled activities per week, consisting of three hours of lectures and a four hour laboratory practical. A pre-lab will be given in VCS3.024 prior to each laboratory session, unless otherwise advised. The lectures cover the content of the unit and lecture notes will be made available after the lecture has been presented. The laboratory sessions are devoted to the attainment of various practical skills and are discussed in detail in the laboratory manual. Lectures

Monday Tuesday Thursday

1.30 pm - 2.30 pm 12.30 pm - 1.30 pm 5.30 pm - 6.30 pm

LBLT KBLT RLT

Laboratory

Wednesday 12.30 pm - 1.30pm LBLT (pre-lab) Wednesday 12.30 pm - 4.30pm LB 3.001, 3.002, 4.002

Brief attendance at other unscheduled times will be required as part of the practical course.

Details on allocations to laboratory classes will be given in lecture 1 and will be posted on LMS in week 1. Your attention is also drawn to the safety provisions for laboratory work that are given in detail in your laboratory manual. Attendance/participation requirements You can only submit laboratory reports if you have attended the laboratory. If you are ill you will be exempted from that laboratory session upon submission of a medical note and you will not be required to submit a report. Your laboratory score will be adjusted accordingly so that you will not be disadvantaged. Please contact Graham to discuss anything related to participation in the laboratory sessions.

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BIO246 MICROBIOLOGY 2020 Unit Information

Week

Day

BIO246 LECTURE TIMETABLE 2020 Lect Topic

Lecturer

Date

1

Mon Tues Thur

1 2 3

Origin, history & relevance of microbiology Prokaryotic cell structure, function & nutrition Environmental factors affecting growth

Graham Graham Wayne

24/2 25/2 27/2

2

Mon Tues Thur

4 5 6

Microbial Growth & division Sterilization & disinfection Antibiotics & chemotherapy

Wayne Wayne Wayne

2/3 3/3 5/3

3

Mon Tues Thur

7 8 9

Microbial Taxonomy Gram Negative bacteria 1 Gram Negative bacteria 2

Graham Graham Graham

9/3 10/3 12/3

4

Mon Tues Thur

10 11 12

Gram Positive bacteria 1 Gram Positive bacteria 2 Identification of bacteria

Graham Graham Graham

16/3 17/3 19/3

Microbial Metabolism 1

Jason

30/3

Microbial Metabolism 2 Microbial interactions

Jason Carolyn

31/3 2/4

Pathogenicity

Carolyn

6/4

Epidemiology and Public Health Disease and Disasters

Carolyn Carolyn

7/4 9/4

ONE WEEK BREAK

5 6

Mon Tues Thur

7

Mon Tues Thur

13 14 15 16 17 18

ONE WEEK BREAK

8 9

10

11

12

13

14

Mon

19

Introduction to viruses

Wayne

20/4

Tues Thur

20

Cultivation & classification of viruses Bacterial viruses – bacteriophage

Wayne Wayne

21/4 23/4

Mon

21

Tues Thur

22 23

Eukaryotic viral diseases Bacterial Genetics

Wayne Wayne

28/4 30/4

Mon Tues

24 25

Functional genomics Archaea and Extremophiles

Wayne Wayne

4/5 5/5

Thur

26

Food Microbiology

Wayne

7/5

Mon Tues

27 28

Bacteria in Terrestrial Ecosystems 1 Bacteria in Terrestrial Ecosystems 2

Jason Jason

11/5 12/5

Thur

29

Introduction to Fungi & Oomycetes

Giles

14/5

Mon

30

Plant-microbe interactions – mycorrhizae

Giles

18/5

Tues Thur

31 32

Plant-microbe interactions – pathogens BioGeoChemistry of C and S cycles

Giles Ralf

19/5 21/5

Mon

33

Microbiology of the N cycle

Ralf

25/5

Tues

34

Applied Environmental Microbiology

Ralf

26/5

Thur

35

Industrial Microbiology

Ralf

28/5

ANZAC DAY

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BIO246 MICROBIOLOGY 2020 Unit Information

Assessment Unit assessment

Laboratory mark Mid Semester Exam Project outline Project work and report Final written exam

20% 15% 5% 10% 50%

Each laboratory report will initially be awarded a mark as shown below: Report 1: Isolation of pure cultures from a water sample Report 2: Viable counting – calculate data & comment Report 3: Epidemiology (completed in class) Report 4: One-step growth of virulent bacteriophage

15 5 5 15

These marks add up to 40 and are scaled to a mark out of 20 for your lab score. Demonstrators will compare their marks on Report 1 before these are returned to you, so that a uniform standard of marking is used for your reports. If you feel that you have been marked unfairly, you should first discuss this with your demonstrator. If you are still dissatisfied, consult with Graham. The Mid Semester Exam will be held on Wednesday 8th April 2020. The laboratory project must be planned and the plan submitted for approval and assessment by Friday of week 10. The project outline contributes 5% to your final assessment. Laboratory work on your project may only commence after the project plan has been approved. The final project report contributes 10% to your overall assessment. Students work in a group of 3 or 4 but must submit an individually written project report, due Friday of week 14. The final examination will be held during the assessment period at the end of the first semester. The exam will assess your understanding of the whole unit. The pass mark for Microbiology is 50%. You are not required to pass individual components of the course to pass the unit. Notation

Grade

Percentage Range

HD D C P N DNS

High Distinction Distinction Credit Pass Fail Fail

S

Supplementary Assessment

80 – 100 70 – 79 60 – 69 50 – 59 Below 50 Fail, the student failed to participate in assessment components with a combined weighting of 50% or more of the final mark. 40 – 49*

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BIO246 MICROBIOLOGY 2020 Unit Information

University policy on assessment Assessment for the unit as a whole will be made in accordance with the provisions of the University Regulations and Assessment Policy. You should check your understanding of the assessment regulations and the associated appeal procedure by referring to the current Murdoch University Handbook. http://handbook.murdoch.edu.au Academic Integrity Murdoch University encourages its students and staff to pursue the highest standards of integrity in all academic activity. Academic integrity involves behaving ethically and honestly in scholarship and relies on respect for others’ ideas through proper acknowledgement and referencing of publications. For information and policies on honesty in assessment including avoiding plagiarism see: http://our.murdoch.edu.au/Educational-technologies/Academic-integrity/ Lack of academic integrity, including the examples listed below, can lead to serious penalties. Find out more about how to reference properly and avoid plagiarism at http://our.murdoch.edu.au/Student-life/Study-successfully/StudySkills/Referencing/Academic-integrity/ Plagiarism

Inappropriate or inadequate acknowledgement of original work including: •

Material copied word for word without any acknowledgement of its source

•

Material paraphrased without appropriate acknowledgement of its source

•

Images, designs, experimental results, computer code etc used or adapted without acknowledgement of the source.

Ghost writing

An assignment written by a third party and represented by a student as her or his own work.

Collusion

Material copied from another student’s assignment with her or his knowledge.

Purloining

Material copied from another student’s assignment or work without that person’s knowledge.

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BIO246 MICROBIOLOGY 2020 Unit Information

Applications for deferred assessment and appeals against unit results. The procedures relating to the lodgement of appeals and applications for deferred assessment are contained in the University's Handbook 2020.

Non-discriminatory language Please refer to http://our.murdoch.edu.au/Student-life/Rights-and-responsibilities/Yourresponsibilities/Non-discriminatory-language-guidelines/

Student appeals Murdoch University encourages students to resolve issues initially through their Unit Coordinator and/or appropriate Faculty staff member. In cases where this is not possible, the University has in place a Student Appeals process. This process is open to all Murdoch University students and there is no fee. The fundamental principles of this process include: 1. 2. 3. 4.

natural justice and procedural fairness; transparency and accountability; the provision of regular procedural review; and the enhancement of the appeals process and outcomes.

An appeal is not a merits based review, in other words, the committee will not reconsider a student’s performance to determine whether a different grade should be awarded. Rather, it is a procedural review and will investigate whether proper process has been followed. In cases where the appeal of a student is upheld by the Student Appeals Committee, the committee will consider what remedy, if any, is appropriate. Students seeking a review of a grade or mark are instead encouraged to follow the procedures set out in the University’s complaint process at http://www.murdoch.edu.au/vco/secretariat/complaints/

Information on the Student Appeals process can be found at http://www.murdoch.edu.au/vco/secretariat/appeals/appeals.html

Student complaints Please refer to http://www.murdoch.edu.au/vco/secretariat/complaints/

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BIO246 MICROBIOLOGY 2020 Unit Information

Resources Textbook The textbook for Microbiology in 2020 is: Willey, J.M., Sherwood, L.M., & Woolverton, C.J. (2017) Prescott’s Microbiology (Tenth Edition). McGraw-Hill. This text provides an extremely valuable resource for the broad discipline that is Microbiology. We would highly recommend students purchase a copy, especially if considering further study and a career involving any aspects of Microbiology. Earlier editions (6th, 7th, 8th & 9th Editions) of Prescott’s Microbiology can also be used as the textbook for Microbiology I. Several copies are available in the University Library. Laboratory Manual You should purchase a copy of the BIO246 Laboratory Schedule which you will require to participate in the laboratory sessions. LMS You will find LMS for Microbiology I to be an extremely useful tool to help you study, provide you with information or exchange ideas and information with fellow students.

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