Detection of VDR gene ApaI and TaqI polymorphisms in patients with type 2 diabetes mellitus using PCR-RFLP method in a Turkish population PDF20160405-1303-1RVC0GL

Title	Detection of VDR gene ApaI and TaqI polymorphisms in patients with type 2 diabetes mellitus using PCR-RFLP method in a Turkish population
Author	Fuat Dilmec
Pages	6
File Size	139.2 KB
File Type	PDF20160405-1303-1RVC0GL
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Journal of Diabetes and Its Complications 24 (2010) 186 – 191 WWW.JDCJOURNAL.COM Detection of VDR gene ApaI and TaqI polymorphisms in patients with type 2 diabetes mellitus using PCR-RFLP method in a Turkish population Fuat Dilmec a,⁎, Elmas Uzer b , Feridun Akkafa a , Elif Kose c , André B.P. van K...

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Detection of VDR gene ApaI and TaqI polymorphisms in patients type 2 diabetes mellitus using PCR-RFLP method in a Turkish population Fuat Dilmec a, , Elmas Uzer b , Feridun Akkafa a , Elif Kose c , André B.P. van Kuilenburg d a Faculty of Medicine, Department of Medical Biology, Harran University, Sanliurfa, Turkey b Faculty of Medicine, Department of Internal Medicine, Harran University, Sanliurfa, Turkey c Faculty of Medicine, Department of Chest Disease, Harran University, Sanliurfa, Turkey d Emma Children's Hospital and Department of Clinical Chemistry, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands Received 9 July 2008; received in revised form 5 October 2008; accepted 3 December 2008 Abstract Type 2 diabetes mellitus (T2DM) is by far the most common type of diabetes and is characterized by insulin resistance a secretion. Some genes, such as the vitamin D receptor gene (VDR, NM_001017535; GI: 7421), involved in its metabolic pa regarded as good candidates for T2DM. In this study, we investigated whether there was an association of VDR: g.59979GNT or c.1 49GNT (ApaIGNT) and g.60058TNC or c.1056TNC (TaqITNC) polymorphisms in the 3 untranslated region of VDR with T2D population. We collected blood samples from 241 individuals (72 patients with T2DM and 169 healthy individuals), and their DNA was isolated. Polymorphisms of the VDR were analyzed by DNA amplifcation with polymerase chain reaction and endonucleas ApaI and TaqI. Body mass index was higher in T2DM patients than in control individuals. However, the frequency of g.599 in T2DM patients was not signifcantly increased compared to healthy subjects (37.5% vs. 36.1%, respectively). Although the VDR g.60058CC genotype in T2DM patients (19.4%) was higher than that in healthy individuals (11.2%), there was no signifca the same way, there was no diference between the groups in allele frequencies. In conclusion, our study did not provide evidence for the association of two examined VDR polymorphisms with T2DM in a Turkish population. © 2010 Published by Elsevier Inc. Keywords: Type 2 diabetes mellitus; Vitamin D receptor gene; Polymorphism; PCR; RFLP 1. Introduction Type 2 diabetes mellitus (T2DM) is one of the most important contemporary medical problems, which increases risks ofcardiovascular disease, kidney failure, blindness, neuropathy and peripheral circulatory disease. It is estimated that the number of people with diabetes worldwide exceeds 200 million, most of them being patients with T2DM. In th societies of the industrialized world, the prevalence of this disease has reached a low percentage of entire population and is still growing (Zimmet, Alberti, & Shaw, 2001). The vitamin D receptor mediates the majority of the efects of vitamin D on gene expression via formation of a heterodimer with theretinoid×receptor, which bindsto promoter regions of many target genes (Uitterlinden, Fang, Van Meurs, Pols,& Van Leeuwen, 2004). The vitamin D receptor(VDR) geneis locatedon chromosome 12 (12q13.11), consists of 11 exons and spans 63495 bp. Sin nucleotide polymorphisms (SNPs) in eight exons (2–9) and three alternatively spliced regions (1a–1c) are distributed functionally relevant areas, including the promoter region Journal of Diabetes and Its Complications 24 (2010) 186 – 191 WWW.JDCJOURNAL.COM Corresponding author. MedicalFaculty,Department of Medical Biology,Harran University, MorfolojiBinasi,Yenisehir Kampusu, TR- 63300, Sanliurfa, Turkey. Tel.: +90 414 312 84 56/24 06; fax: +90 414 313 96 15. E-mail addresses: [email protected], [email protected] (F. Dilmec). 1056-8727/08/$ – see front matter © 2010 Published by Elsevier Inc. doi:10.1016/j.jdiacomp.2008.12.002...