DNA Lab Homework PDF

Title	DNA Lab Homework
Course	General Bioloy: Cell Bioloy and Physioloy
Institution	University of Southern California
Pages	1
File Size	162.1 KB
File Type	PDF
Total Downloads	85
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Summary

BISC 220 Lab on DNA...

Description

DNA LAB HOMEWORK Below is a pGEN 101 plasmid with restriction sites for BamHI and EcoRI. Several restriction digests were done using these two enzymes either alone or in combination. Kb stands for kilobases, or thousands of base pairs.

a. Which lane shows a digest with BamHI only? _____ b. Which lane shows a digest with EcoRI only?

_____

c. Which lane shows the fragments when the plasmid was cut with both EcoRI and BamH1? _____ d. What is the size of the uncut plasmid? ______________ Below is another plasmid, pBR322. If you digest this plasmid with SalI, EcoRI and NdeI, how many fragments would you get and what size would they be?...