DNA Worksheet Lab PDF

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Exercise 4 – DNA Isolation/Forensics Worksheet Name: Date: 10/24/17

Lab Section: 065

1. Sketch the patterns of DNA fragments that you observed on your gel (or insert a photo of your gel from lab). Be sure to identify which sample is in each lane

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Lane 1: Standards, Lane 2: Crime Scene sample, Lane 3: Suspect #1, Lane 4: Suspect #2, Lane 5: Suspect #3

2. Is there a match between any of the suspects and the DNA collected at the crime scene? Based on this limited amount of information, who do you think might have killed Jessica? Do you have enough information to convict a suspect? Why or why not? Yes, Suspect #3 had a match to the DNA found at the crime scene. I believe that Suspect #3 might have killed Jessica. I do not think that there is enough information to convict Suspect #3. There could be various alternative explanations for this to match the suspect at the crime scene. There is always a an extremely small chance that the DNA from Suspect #3 coincidentally had the same DNA as an unrelated person, the DNA is from a close relative of Suspect #3, there was an error in the lab procedure, or the DNA was put on the crime scene purposely.

3. DNA fingerprinting permits subtle differences between individuals to be identified at the genetic level. Two applications that demonstrate how DNA fingerprinting is useful include paternity testing as well as forensic science. Go to the following website and explore the DNA/Criminal Justice link: https://koshland-science-museum.org/sites/all/exhibits/exhibitdna/index.jsp Using this and other reliable web sources, answer the following question: How can FBI CODIS system be used to identify or exonerate a suspect in a criminal case? The FBI CODIS is based entirely on probability. There are thirteen regions of the genome that are probed with this FBI system. The FBI focuses on the easily detectable differences that are in the repeats of the DNA sequences of these regions. If both of the DNA samples match perfectly in all of these regions, then it is highly likely that the criminal has been found. If the samples do not match, then the suspect can be exonerated. The probability of two different people to have a perfect match of DNA is essentially zero. Furthermore, the FBI use CODIS by using the sample from a crime scene and takes its DNA profile. They use their DNA database to see if there are any matches with this DNA....