E Science Lab 0.2 General Lab Safety C PDF

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General Lab Safety BIOL 101: General Biology Name: Semester: Lab 0.2: General Lab Safety (1 hr.) - 5 points

General Lab Safety Pre-Lab Questions (1 POINT/.25 POINTS EACH) 1. What should you always wear to protect your eyes when you are in a laboratory? 

Safety glasses

2. Should you add acid to water or water to acid? 

Add the acid to the water to dilute. Never add water to acid

3. Where should you dispose of broken glass? 

Using a broom and dustpan, clean it up and dispose of in waste receptacle

4. What should you do if you spill a chemical on your hand? 

Immediately wash hands with soap and water

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General Lab Safety Exercise 1: What Is It? (1 POINT) Laboratories contain specialized equipment used while performing experiments. Locate each item pictured on this page in your lab kit, and place a check mark in the appropriate place when you find it. After you have completed this checklist, sketch 5 additional items in your lab kit that will be useful in completing the labs that follow.

Beaker 50 mL ☐ 100 mL ☒

Test Tube ☒

Stir Stick ☒

Graduated Cylinder 10 mL ☒ 100 mL ☒

Pipette ☒

Petri Dish ☒

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General Lab Safety Sketch, take a picture, or find a picture online of five additional pieces of labware not shown on the previous page in the space provided below: (1 POINT)

by Unknown Author is licensed under

by Unknown Author is licensed under

by Unknown Author is licensed under

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General Lab Safety Experiment 1: Neutralization of Acids and Bases (1 POINT) Data Tables Table 2: Initial pH Test Results Container

Chemical Contents

Initial pH

A

5ML water

7

B

10 ML HCl

1

C

100 ML water + 0.5 g NaHCO3

3

Additional Observations

Table 3: Neutralization of Acid Total Amount of NaHCO3 Added

Beaker C pH after adding acid

0.5 (initial solution)

4

1.0

5

1.5

7

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General Lab Safety Post-Lab Questions (1 POINT) 1. Most of the chemicals included in your kit can be discarded down a drain with copious amounts of water. Describe a situation in which you would need to neutralize a chemical before discarding down a drain.



When the chemical is extremely acidic or extremely basic. Also, some chemicals can mix with other organic or inorganic compounds create an issue. It is best to always neutralize anything before discarding, and if there are any questions, research for proper and safe disposal.

2. Why should you add acid to water rather than add water to acid when preparing solutions?



Adding acid to water makes a diluted concentration rather than having a strong acid and slowly diluting it with water.

3. At what point was the solution in beaker C neutralized?



After adding 1.5g of sodium bicarb to the solution

4. Address the following scenarios:

a. If a more concentrated initial solution of sodium bicarbonate was used in beaker C, would it require more or less bicarbonate to neutralize the acid? Why?

o

It would require more to neutralize due to the release of CO2 as NaHCO3 and HCL.

b. If a less concentrated initial solution of sodium bicarbonate was used in beaker C, would that solution require more or less bicarbonate to neutralize the acid? Why?

o

Less due to the smaller amount that needed to react with each other

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