Egel 1kb plus express ladder man PDF

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Choosing the right DNA ladder for your E-Gel™ agarose gel Agarose %

E-Gel™ Single Comb

E-Gel™ 50 bp DNA Ladder

E-Gel™ 1 Kb Plus DNA Ladder Cat. No. 10488090

E-Gel™ 1 Kb Plus Express DNA Ladder Cat. No. 10488091

E-Gel™ Sizing DNA Ladder Cat. No. 10488100

E-Gel™ Low Range Quantitative DNA Ladder Cat. No. 12373031

E-Gel™ 96 High Range DNA Marker

Cat. No. 10488096

Cat. No. 10488099

1%

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2%

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4%

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1.2%

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2%

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0.8%

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1.2%

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2%

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4%

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E-Gel™ EX

E-Gel™ with SYBR™ Safe

E-Gel™ Ultra Low Range DNA Ladder

Cat. No. 12352019 

1%

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2%

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E-Gel™ CloneWell™ II

0.8%

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E-Gel™ SizeSelect™ II

2%

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0.8%

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1%

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2%

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1%

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E-Gel™ Double Comb

E-Gel™ NGS ™

E-Gel Go!

E-Gel™ 48

E-Gel™ 96 E-Gel™ 96 SYBR™ Safe

2%

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4%

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1%

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2%

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1%

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Recommended DNA ladder

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Compatible DNA ladder

Print Options

Gel type

Important licensing information This product may be covered by one or more Limited Use Label Licenses. By use of this product, you accept the terms and conditions of all applicable Limited Use Label Licenses.

Limited product warranty Life Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies’ General Terms and Conditions of Sale found on Life Technologies’ website at www.thermofisher.com/us/en/home/global/terms-and-conditions.html.

Disclaimer TO THE EXTENT ALLOWED BY LAW, LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.

Corporate entity: Life Technologies | Carlsbad, CA 92008 USA | Toll Free in USA 1.800.955.6288 ©2017 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.

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Prepare and load DNA ladders and samples This protocol provides a brief description of how to use the DNA ladder with E-Gel™ agarose gels. For detailed instructions on using specific types of E-Gel™ agarose gels, go to thermofisher.com or contact Technical Support.

Step

Action a. Thaw, mix and briefly centrifuge DNA ladder before use. b. Prepare DNA ladder.

1

2

Prepare DNA ladder

Prepare samples

∤ ∤ ∤ ∤

For E-Gel™ CloneWell™ II Agarose Gels, mix and use 25 µL of the ladder without dilution. For E-Gel™ EX Agarose Gels, mix 2 µL of DNA ladder with 18 µL of water. For E-Gel™ Agarose Gels, mix and use the ladder without dilution. For E-Gel™ 48 Agarose Gels, mix 2 µL of DNA ladder with 13 µL of water.

a. Dilute your sample 2- to 10-fold with TE Buffer (Cat. No. AM9858), 1X E-Gel™ Sample Loading Buffer (Cat No. 10482055), or water. b. Mix gently. a. Load DNA ladders and DNA samples into the appropriate wells of the E-Gel™ agarose gel.

∤ 3

Load samples and DNA ladders

Add 25 µL for E-Gel™ CloneWell™ II Agarose Gels. Note: All wells of E-Gel™ Clonewell™ II Agarose Gels must be pre-filled with 50 µL of water.

∤ ∤

Add 20 µL for E-Gel™ and E-Gel™ EX Agarose Gels. Add 15 µL for E-Gel™ 48 Agarose Gels.

b. Add water to any empty wells, so that all wells contain an equal volume of liquid. c. Proceed to Perform electrophoresis of E-Gel™ agarose gel.

For support, visit thermofisher.com/support. 3 September 2017

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Perform electrophoresis of E-Gel™ agarose gel This protocol provides a brief description of how to perform electrophoresis with E-Gel™ agarose gels. For detailed instructions on using specific types of E-Gel™ agarose gels, go to thermofisher.com or contact Technical Support.

Step

Action ™

a. Choose the appropriate E-Gel run protocol for your gel type based on the electrophoresis device being used. Gel type Program E-Gel™ Power Snap Electrophoresis Device (Cat. No. G8100)

4

Perform electrophoresis

E-Gel™ Clonewell™ II Agarose Gel (0.8%) E-Gel™ EX Agarose Gel (1%, 2%)

Recommended run time

CloneWell 0.8%

12 min (40 min max)

E-Gel EX 1-2%

10 min (20 min max)

E-Gel 0.8-2%

26 min (40 min max)

E-Gel™ Agarose Gel (0.8%, 1.2%, 2%) E-Gel™ Double Comb Agarose Gel (1%, 2%) E-Gel™ E-Base™ Device E-Gel™ 48 Agarose Gel (1%, 2%)

E-Gel Double Comb

13 min (20 min max)

EG

20 min

b. Run the program to start electrophoresis. Visualize DNA ladder and samples.

the E-Gel Power Snap Camera (Cat. No. G8200), E-Gel Imager (Cat. No. · Use 466612), or other blue light imager to detect DNA bands stained with SYBR ™

5

Visualize agarose gel

™

™

stains.

· UV transilluminator to detect DNA bands stained with ethidium bromide.

For support, visit thermofisher.com/support. 3 September 2017

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