Enzyme Activity Worksheet Module 6 PDF

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Enzyme Activity Worksheet...

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M6 ENZYME ACTIVITY ASSAY WORKSHEET

NAME: Troy Estigoy

SECTION: 3

1. What is the difference between anabolic and catabolic activity? (2pts) Catabolic breaks down a substrate into two or more products, while anabolic activity creates a product with two or more substrates.

2. List four variables may affect enzymatic activity (2pts) Four variables that may affect enzymatic activity is time, amount of substrate, temperature, and pH.

3. In an enzymatic reaction, if the substrate concentration is increased, why would the generated products tapper into a plateau? (2pts) If the substrate concentration were to be increased the generated products would start to plateau once the reaction reaches its limit, once this occurs the reaction rate will gradually decrease.

4. What is an assay? (2pts) Assay is a laboratory analysis completed on a substance, cells, or body fluid that allows scientist to see the presence or amount of a target substance present or if the target substance has been altered. Examples: testing enzyme activity, types of blood testing, testing for presence of proteins, and testing antiviral treatments.

5. What is a spectrophotometer? (2pts) A spectrophotometer is a tool used to analyze absorbance of a substance at a certain wavelength.

6. If you generate a standard curve, do you do this to quantify your reaction products or substrates? (2pts) A generated standard curve would quantify your substrate concentration.

M6 ENZYME ACTIVITY ASSAY WORKSHEET

7. What are the five steps in generating a standard curve? (2pts) The five steps in generating a standard curve is: 1) Assaying substrate of known concentration 2) Obtaining absorbances for these known concentrations 3) Creating a scatter plot with x-axis: substrate concentration; y-axis: absorbance 4) From that scatter plot generating a tread line in y= mx + b form 5) Running samples of unknown concentration obtaining the absorbance and use that as your yvariable and solve for x which will be your substrate concentration.

8. A flow cytometer allows you to analyze the ___________________ of single cells. (1pts) Fluorescence.

9. A western blot is conducted to visual proteins. This allows you to confirm __________ and _________ of proteins (2pts) Expression; Size

10. Explain the difference between in cis and in trans enzymatic activity. (2pts) The difference between in cis and in trans enzymatic activity is that in cis indicates that the protein can act as both a substrate and a protein, while in trans concurs that there are two different compounds interacting, concluding that there is a separate substrate and protein.

11. In the cellular assay for repurposing drugs for protease inhibitors, when the cells are green does that mean the protease is active or inactive? When the cells are green it indicates that the protease is active....