Factors affecting enzyme activity PDF

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Notes in biology A Level OCR A...

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Factors affecting enzyme activity

Chemicals and temperature

• As temperature increments so does motor energy and the quantity of effective crashes expanding the pace of response

• Optimum temperature, this is when chemical movement is at its most elevated

• Temperature excessively high, vibrations break bonds that hold the protein together. Chemicals denature (irreversible change to shape the dynamic site)

•

Enzyme movement diminishes.

Temperature coefficient Q10

• The Q10 an incentive for a response shows how much the pace of response changes when the temperature is raised by 10oC

• At temperatures before the ideal, a Q10 worth of 2 implies that a rate copies each 10oC increment. 3 methods it high pitches.

• Most have a

catalysts Q10 of 2

Ascertain Q10

Q10 = rate at higher temperature

rate at lower temperature

E.g

The pace of a specific response is 10 units/min at 20 °C and 24 units/min at 30 °C. What is its Q10?

Q10 = rate at higher temperature ÷ rate at lower temperature

= 24 ÷ 10 = 2.4

Chemicals and pH

•

The ideal pH – the pH the chemical works best at

• As you move away for the ideal pH, chemicals begin to denature and compound action diminishes.

• H+ and OH-particles found in acids and antacids break the ionic and H bonds that hold the compound's tertiary design set up...