Identifying gram Flow Charts PDF

Preview

Summary

flow chart for identifying microbes...

Description

Gram-positive Cocci

Gram-positive Cocci

Catalase Negative Flow Chart

Catalase Test

+

–

Micrococcaceae

Streptococcaceae Hemolysis

Beta β

Alpha α

GpC Strep GpA Strep (S. pyogenes)

Enterococcus Strep mitis

Bacitracin Sensitivity

Bile Esculin Test

+

–

+

–

GpA Strep (S. pyogenes)

GpC Strep

Enterococcus

Strep mitis Strep pneumoniae

Optichin Sensitivity

+

–

Strep pneumoniae

Strep mitis

Gram-positive Cocci

Gram-positive Cocci

Catalase Test

Catalase Positive Flow Chart

Coagulase Test

+

–

Micrococcaceae

Streptococcaceae

Hemolysis

Mannitol Fermentation Test

+

–

+

–

Staphylococcus aureus

Staphylococcus epidermidis Micrococcus luteus Micrococcus roseus

Staphylococcus aureus

Staphylococcus epidermidis Micrococcus luteus Micrococcus roseus

Growth on Mannitol Salt Agar Test (MSA)

Beta β

Alpha α

Staphylococcus aureus

Staphylococcus epidermidis Micrococcus luteus Micrococcus roseus

Pigmentation Observation

Growth on MSA or Pigmentation Observation

+

–

Grey/White

Golden/White

Mustard Yellow

Pale Pink/Rose

Staphylococcus aureus Staphylococcus epidermidis

Micrococcus luteus Micrococcus roseus

Staphylococcus epidermidis

Staphylococcus aureus

Micrococcus luteus

Micrococcus roseus

See Pigmentation Observation to differentiate

Gram-negative Rods

Gram-negative Rods

Lactose Positive Flow Chart

Lactose Test (KIA or Carbohydrate Fermentation Test)

+

_

IMViC Tests

++-E. coli

--++

Motility

+ Enterobacter aerogenes

Klebsiella

Gram-negative Rods

Gram-negative Rods

Lactose Test

Lactose Negative Flow Chart

(KIA or Carbohydrate Fermentation Test)

+

_

Oxidase Test

+

_

Gelatin Test

H2S (KIA) Test

+ Pseudomonas aeruginosa

-

+

Shigella flexneri

Alcaligenes faecalis

Urea Test

+ Proteus vulgaris

Salmonella typhimurium...