Lab 1 Muscle Fatigue Complete PDF

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BIOL 1124, Lab 1: Applying Scientific Process to Studying Muscle Fatigue You will apply the scientific process to a study of muscle fatigue. Develop and conduct your study as a team, however each individual should complete this sheet. You will develop a hypothesis about the effect of an independent variable at two levels on muscles and the level of muscle fatigue. Your dependent variable will be the number of times the clothespin can be squeezed in 1 minute (or other reasonable time period) trials. Your Materials include the clothespin, paper, pen, timer. Here’s some information about the system so that you can formulate a hypothesis: - Muscle cells rely on aerobic (with oxygen) respiration for their energy needs. - Breathing brings oxygen into the body; blood delivers it to muscle tissues and cells. - If muscles are being used and their oxygen demands exceed the supply they will begin to use anaerobic metabolism (no oxygen) which produces lactic acid as a waste product. - Therefore, after oxygen supplies have been used up the result will be muscle fatigue, lactic acid and an accompanying burning sensation. - Fatigued muscles have reduced capacity for contraction until the lactic acid has been removed from the tissue by natural processes (~10 minutes). - Muscle mass varies by person, and by part of the body, e.g. by hand (with handedness) and by finger. - Exercise increases the heart rate and blood flow throughout the body, including extremities. Work with your lab team to answer the following questions and develop your study. Your methodology should minimize bias and error. Be prepared to share! First, design your study by answering questions 1-4. You may want to consider using scrap paper instead of this sheet while you work out your study. 1. What is the hypothesis that you will be testing? Provide your reasoning. If a clothes pin is squeezed with your index finger and thumb during consecutive trials then the number of the squeezes per trial will decrease because of muscle fatigue caused by lactic acid build up.

2. What is the independent variable and the two levels you are testing? The number of consecutive trials

3.What is the dependent variable? The number of squeezes 4. METHODS: Briefly describe the methodology for measuring the dependent variable including who will do what (e.g. squeeze the clothespin, keep time, record seconds, etc.). One member will be squeezing the clothes pin and keeping track of the number of squeezes. This will be done three times at a full minute with 10 seconds in between. Member will be using dominate right hand with index finger and thumb. That member will be the only Person squeezing will be the only person squeezing as many times as possible from fully open to fully closed. A separate member will be recording the time and the last member will record throughout the experiment.

5. Now check your proposed study with the instructor, then conduct your study (perhaps with modifications). 6. RESULTS: Use this space to make a table to record your data. Include a title, and units of measurement. Make sure to calculate the average for the data from each group (each level of your independent variable). Test 1 # of Squeezes

Test 2 # of Squeezes

Average # of Squeezes

Trial 1

191 squeezes

206 squeezes

198 squeezes

Trial 2

163 squeezes

168 squeezes

165 squeezes

Trial 3

126 squeezes

138 squeezes

132 squeezes

7. DISCUSSION (Conclusions): Did the results support the hypothesis? _______________. Either way, explain what your results say about your study, and the system you studied. What do you your results mean? Be ready to present your study and findings to the class. The hypothesis was supported by the data, after every trial the number of squeezes when down along with the average for the trials. Trial 1 had an average of 198 squeezes and the trial 3 had an average of 132 which supports the thought hypothesis that lactic acid built up causes muscle fatigue.

Modified by K. Miller, MxCC from https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&ved=0ahUKEwi4ZL32_PPAhVK94MKHZwPDUEQFggfMAA&url=http%3A%2F%2Fwww.tamdistrict.org%2Fcms%2Flib8%2FCA01000875%2FCentricity%2FDomain %2F681%2FMuscle%2520FatigueLab2014.doc&usg=AFQjCNF2miB36f8-CWrWelq1Q-Jm8nS-CQ&bvm=bv.136593572,d.cWw&cad=rja...