LFS112 - Workshop Manual PDF

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Coordinators: Associate Professor Shaun Sandow and Dr Mia Schaumberg...

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LFS112 Human Physiology Workshop Manual Coordinators: Associate Professor Shaun Sandow and Dr Mia Schaumberg

School of Health and Sport Sciences

Semester 2, 2019

university of the sunshine coast

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School of Health and Sport Sciences The University of the Sunshine Coast

LFS112 Human Physiology Workshop Manual Tutorial and Laboratory Classes Course Coordinators: A/Prof Shaun Sandow and Dr Mia Schaumberg Head Tutor:

Dr Felicity Lynch

Semester 2, 2019

Your Name:

………………………….……….….

Tutorial Class Time and Room:

…….…………………….…………. Laboratory

Class Time and Room: Name of your Tutor: LFS112 Human Physiology

…….…………………….…………. ..………………………….………… Semester 2 2019

Attach evidence of completion of Science Labs Induction Sippy Downs here. Semester 2, 2019

ACKNOWLEDGMENTS: all figures in this manual are from Martini et al (2019) LFS112 course text, unless otherwise indicated. This manual was prepared by current and past LFS112 teaching staff.

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Contents Introduction................................................................................................................................................ 4 Schedule of Submissions............................................................................................................................. 7 Laboratory Rules......................................................................................................................................... 8 Safe handling of Blood / other Body Substances....................................................................................... 15 Week 1: Tutorial 1: Course Introduction, Thermoregulation and the Integumentary System....................19 Week 2: Laboratory 1: Introduction to Powerlab and LabTutor.................................................................32 Week 3: Tutorial 2: Neurophysiology and Muscle Physiology....................................................................50 Week 4: Laboratory 2: Blood Glucose Analysis.......................................................................................... 61 Week 5: Tutorial 3: Endocrine System and Cellular Metabolism...............................................................75 Week 6: Laboratory 3: Mid-semester Exam and Self-directed Activities to Review Haematology.............87 Week 7: Tutorial 4: Review of Immune System and Digestive Physiology..................................................92 Week 8: Laboratory 4: Cardiac Physiology............................................................................................... 102 Week 9: Tutorial 5: Cardiovascular and Renal Physiology........................................................................ 121 Week 10: Laboratory 5: Respiratory Physiology...................................................................................... 130 Week 11: Tutorial 6: Respiratory physiology and Reproductive physiology.............................................142 Week 12: Laboratory 6: Sensory Physiology............................................................................................ 151 Week 13: No formal scheduled class....................................................................................................... 165

Consent to Participate in Laboratory Activities………………………………………………………………………………………166

Consent forms to participate in laboratory classes can be found at the end of this manual. Where relevant, sign the form, remove the page from your manual and present it to your laboratory tutor.

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Introduction

Human Physiology covers the normal mechanisms of function in the body. It includes topics on metabolism, digestion, osmoregulation, excretion, respiration, circulation, muscles, neurophysiology, sense organs, immunology, endocrinology and reproduction. Emphasis in the course is on the normal function of the body and the approach aims to develop an understanding of the integrative nature of physiological systems. The course (described in the below Figure) consists of 1 x theoretical eModule (to be completed PRIOR to attending classe), 2 x 1-hour lectorials / lectures per week starting in week 1 (ensure you have also purchased the lectorial Workbook), a 2-hour tutorial class in alternate weeks starting in week 1, and a 2hour laboratory class in alternate weeks starting in week 2. In addition, this course includes online activities as directed.

Theory (eModules and Lectorials) Prior to attending the weekly lectorial, you are required to complete the online eModule, where the theoretical content is delivered. The live lectorials will direct your revision of key concepts from the theoretical eModules. LECTORIALS ARE NOT A REPEAT OF THE THEORETICAL CONTENT and as such you will need to engage with BOTH the eModule AND lectorial. You are strongly advised to attend the lectorials (or make up the time viewing and listening to the lectures using the Mediasite recording on Blackboard), so that you can complete the associated activities in the lectorial workbook which will be essential for your revision and success in the course. Mediasite recordings of the lectures are made and posted to the Learning Activities area of Blackboard. However, please keep in mind that technical glitches can occur and so we cannot guarantee that each recording will be available. Completing the lectorial before your tutorial and laboratory class is expected. You are reminded that LFS112 is optimally not an online course and so active participation in all classes, including lectures, tutorials and lab classes is strongly recommended and failure to attend and participate is more than likely to result in a less than desired performance in the course, including a fail.

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Workshops (Tutorials and Laboratory Classes) Attendance at tutorial and laboratory classes is necessary for your learning of key physiology concepts and skills. You should prepare for these classes by reading the notes beforehand, completing any set readings or preparatory questions and revising the relevant lecture material. Class attendance will be recorded, and marks are given for preparation (satisfactory completion of pretutorial or pre-laboratory questions and any post-lab work assigned) and participation (satisfactory engagement and participation in group activities in tutorial or classes). Permission from a Course Coordinator ([email protected]) is required if you wish to attend a class other than the one in which you are enrolled. Please note that classes are only scheduled on Tuesday, Wednesday and Thursday.

Consolidate and apply Following the directed class activities, post-class activities, including revision questions (in the Lectorial Workbook) and directed assessment activities (available on Blackboard) are available to be completed. Keep an eye out for reminders in this Manual to help you keep track of this.

Absence from Tutorial and Laboratory Classes If you are absent from class and you want the opportunity to gain preparation and participation marks, you must submit an AAE (Application for Assessment Extension) to request an extension. The instructions and form to apply for an AAE are available in the Assessment: Tasks, Extensions and Procedures tab of the LFS112 Blackboard site. AAE forms should be submitted to a course coordinator using your USC Student Email address and sent to [email protected] Upon approval from a Course Coordinator, an email will be sent requesting that you complete the necessary work, which you must then show to your tutor at the next tutorial / laboratory class or by the due date, per the email.

Things to remember for laboratory classes 1. Write your name on the front and in your workshop manual and bring it to every class. 2. Complete the compulsory induction for the Science Labs - Sippy Downs , accessed via Lab Induction tab: USC Laboratories and Inductions 2019 on Blackboard. Print out your certificate showing your 100% score and show your tutor at the next class who will mark it on the roll. Completion of the Science Lab induction is a strict OHS requirement and failure to complete the induction will result in exclusion from the class/es and therefore loss of marks.

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LFS112 Human Physiology Semester 2 2019 3. Bring the following personal protective equipment (PPE) to lab class: laboratory coat, safety glasses, and closed-in, non-slip footwear that covers the heal and top of the foot. Be sure to label your lab coat and safety glasses. Lab coats, safety glasses and gum boots can be borrowed up to a limit.

If you have long hair remember a hair tie so you can tie it back. 4. Please note that phones, tablets and laptops are NOT generally allowed into the laboratory class for safety and hygiene reasons.

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Schedule of Submissions You may find the schedule below helpful throughout semester. Check it regularly to see when assessment tasks are due and tick them off as you complete them. Week

Lecture

Class

Assessment task submission

Due/ Done

1

Course introduction; Thermoregulation & the Integumentary system

Tutorial 1: Course introduction, Thermoregulation & the Integumentary system

Formative cell biology and chemistry diagnostic revision quiz

☐

2

Neurophysiology

Laboratory 1: Introduction to Powerlab & Labtutor

Task 1A: Pre-lab questions & class participation [formative]

☐

3

Muscle physiology

Tutorial 2: Neurophysiology & Muscle physiology

4

Endocrine system

Laboratory 2: Blood glucose analysis

Task 1A: Pre-tutorial questions & ☐ class participation [1 mark] Completed Excel graph from online graphing eModule [1 mark] Task 1A: Pre-lab questions & class ☐ participation [1 mark]

5

Cellular metabolism

Tutorial 3: Endocrine system & Cellular metabolism Laboratory 3: Selfdirected activities in Haematology

Task 1A: Pre-tutorial questions & class participation [1 mark] Task 1B: Lab Quiz 1 [4 marks]

☐

Task 1A: Laboratory 2 data presentation [2 marks] Task 2: Mid-semester exam [25 marks]

☐

Task 1A: Pre-tutorial questions & class participation [1 mark]

☐

6

Blood physiology and the Immune system

7

Digestive physiology

Tutorial 4: The Immune system and Digestive physiology

8

Cardiac and Vascular physiology

Laboratory 4: Task 1A: Pre-lab questions & class Cardiovascular physiology participation [1 mark]

9

Renal physiology

Tutorial 5: Cardiovascular & Renal physiology Laboratory 5: Respiratory physiology

10

Respiratory physiology

Break 11

Reproductive physiology

12

Sensory physiology

13

Revision Lecture

Teaching free period Tutorial 6: Respiratory Task 1A: Pre-tutorial questions & physiology & class participation [1 mark] Reproductive physiology Laboratory 6: Sensory Task 1A: Pre-lab questions & class physiology participation [1 mark] No formal class: drop in catch-up opportunity

Total marks 7 Semester 2, 2019

Task 1A: Pre-tutorial questions & class participation [1 mark] Task 1B: Lab Quiz 2 [8 marks] Task 1A: Laboratory 4 data presentation [3 marks] Task 1A: Pre-lab questions & class participation [1 mark]

☐

☐ ☐ ☐ ☐ ☐

☐ ☐

Task 1B: Lab Quiz 3 [8 marks]

☐

[60 marks]

☐ ..…….

LFS112 Human Physiology achieved during semester

Semester 2 2019

Laboratory Rules The following material has been supplied by the USC Risk and Fieldwork Manager and we highlight it for you to read and be aware of.

Introduction Laboratory sessions are an important aspect of the learning and teaching processes at USC, as they enable you to apply knowledge and gain valuable practical skills in the laboratory environment. Many of USC courses include laboratory sessions and students are encouraged to embrace this unique learning experience. Some laboratory classes have assessments for your final grades. You are reminded that skills or experience in the laboratory environment are often required/desired by employers for many jobs in the Science, Health and Education disciplines. To make the most of the laboratory activities it is necessary to be aware of and adhere to certain safety rules and regulations. This is to ensure the safety of yourself and all other laboratory users.

Responsibilities USC is always committed to managing the health and safety of all staff and students, including whilst conducting laboratory activities. The many associated risks of laboratory activities must be managed by both the organizers and the participants. Your responsibilities, as a student participating in laboratory sessions, are listed below. You must be familiar with and adhere to them.

As a student carrying out teaching activities in a laboratory class you must: •

understand and comply with your obligations, to conduct work safely and not compromise the safety of others (as specified by WHS legislation).

•

understand and adhere to relevant USC guidelines, policies and procedures.

•

be aware of and adhere to laboratory rules.

•

be aware of the hazards and control measures for the laboratory activities in each session.

•

notify the Lab Supervisor of any special consideration or needs (e.g. medical conditions which may increase your risk) prior to participating in the laboratory sessions.

•

follow all safety instructions from the Laboratory Supervisor and technical staff.

Governing regulations: The work conducted in laboratories is highly regulated through standards and legislation (WH&S Act, 2011) due to the specific risks and hazards involved. These standards (AS/NZ 2243 [type 2243 into search box to access]) dictate the minimum standards and practices to be conducted within laboratory spaces. 8 Semester 2, 2019

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Laboratory Rules: • • • • •

•

Always follow instructions given by the laboratory supervisor, tutor, or technical staff. Always tie back long hair (longer than shoulder length). Always wash hands on entering and before leaving the laboratory. Always protect reading and writing materials/keyboards from contamination (remove gloves). Always apply waterproof covering to any skin breaks (e.g. band-aids). Some are available in first aid kits in the room. Any skin breaks that occur during the session must also have an incident report completed. Bags and personal belongings (e.g. laptops) must be placed in the student bag racks or lockers.

The following list includes things you are not permitted to do in the laboratory: • • • • •

consume food or drinks (you must go outside). bring food or drinks into the laboratory (leave them in your bag in the back racks or lockers). place pens or pencils in your mouth. apply cosmetics, including lip balm. handle contact lenses (in addition, you should not wear contact lenses in the laboratory if chemical vapours are present).

The following rules are mandatory for all laboratory sessions. You must: •

• •

•

• • • • •

wear your laboratory coat fully buttoned, before entering and at all times in the laboratories and remove it before leaving the laboratory (keep it in a plastic bag if carrying in your bag/backpack). DO NOT WEAR around campus. wear closed, non-slip footwear that covers the heel and top of the foot. The correct protective safety glasses/goggles that fit close to the face to prevent splashes into the eye, must be worn in the laboratory at all times, unless otherwise directed by the laboratory supervisor. wear gloves when handling body fluids, microbial cultures or chemicals. Remove gloves and dispose of them in the yellow biohazard bins before leaving the lab. handle all sharps such as dissection instruments, razors, glass slides and cover-slips with care. volatile and odorous compounds must be used in an operating fume hood. follow directions in laboratory manuals and from the laboratory supervisor and technical staff. report all spills to your laboratory supervisor so the spill can be contained and cleaned appropriately. Refer to the laboratory spill procedures, tutors and technical staff for assistance. Always keep laboratory doors closed. Only students and staff associated with the teaching activity are allowed access to the laboratory during the laboratory session. Students are not allowed in the laboratory without a tutor present. 9

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Handwashing: For general work in the teaching laboratory the following method of hand washing should be applied. You should wash hands before and after use of the disposable gloves, and at the start and end of your laboratory session.

PPE: Personal Protective Equipment: Personal Protective Equipment is equipment or clothing designed to protect you from the risk of illness or injury when working in the laboratories. You may be asked to leave the laboratory if you are not wearing the mandatory PPE. Mandatory PPE in the USC laboratories is: 1. Laboratory Coat (fully buttoned up).

2. Safety Glasses, as; These must fit close to the face and not slip forward away from the face when you look down. Gaps between your face and the glasses could permit splashes of chemicals or blood to enter your eyes. Safety glasses can be purchased from the Co-op shop (at the Sippy Downs campus) or safety retail outlets.

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A sports lanyard with adjustment will prevent glasses sliding off your face when you look down.

This design si works well over prescription glasses– ensure the safety glasses fit close to

3. Closed in Shoes (please refer to the diagram below for correct footwear).

Biological hazards: Also refer to “Safe Handling of Blood and Other Body Substances” in the student practical manual. All blood, fluid, tissue samples and microbial samples you work with in the laboratory should be regarded as potentially infectious and can cause disease regardless of the presumed infectious status of a person/anima...