Micro Lab Final Study Guide 245 1004 PDF

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micro lab practical final. Study guide used as test bank, all practicum questions taken from study guide...

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Re vi e wGui def orBI O245La bPr a c t i c a lEx am Thi si sj us tagui de ;i ti sno tane x haus t i v el i s t .Anyt o pi cc o v e r e di nc l as sc anpo t e nt i al l ys ho wup o nt hepr a c t i c al . Thee xa m wi l lbeawr i t t e nonewi t h25s t at i ons( e a c hwo r t h4p oi nt s )f orat ot a lof10 0po i nt s .You wi l lb epr o v i de dwi t hap ar t i c ul ars c e nar i oanda s ke dt oi de nt i f yt het e s t , i ndi c at et her e s ul t sand p r o v i d ea ni nt e r pr e t at i on( asr e l e v ant ) . 1 .Bea bl et oi d e nt i f yGr a mp os i t i v ea ndGr a mn e g a t i v eba c t e r i a ,a l s ot oi de nt i f yr o d, c oc c u s , a nd s pi r a ls ha pe s .Wh a ta r et h er e l a t i v ea dv a nt a g e sofwe tmo un t sv e r s u ss t a i ns ?Wh a to t he rt y pe sof s t a i n s( b e s i de sGr a m)a r et he r ea ndwha ta r et h e yf or( e v e ni fwed i dn otc o v e rt h e mi nt h el a b ,b ut c ou l dbeus e f ulf o ri d e nt i fic a t i o n) ? 2 .Kno wt heme di awer e gu l a r l yu s ei nl a ba ndwha te a c hi sus e df o r :TSA,EMB, a g a rd e e pse t c . Be a bl et od i s t i n gu i s hap os i t i v ef r omane g a t i v et e s t , whe r ea p pr op r i a t e . 3 .Kno wt hedi ffe r e n c e sbe t we e ns e l e c t i v e ,di ffe r e nt i a l ,de fin e d ,a n dun de fine dme di a . 4 .Kno wh o wt op e r f o r ma ndi n t e r p r e ta l lt het e s t swedi dt hi ss e me s t e re v e ni fy o udi d n ’ tpe r f o r ma l l o ft he my ou r s e l f .Exa mp l e si nc l ud e :KOH,c a t a l a s e ,o xi da s e ,MR/ VP ,SI M, e t c … Ex pl a i nwh e na nd wh ye a c hwou l db eus e d . 5 .Kno wh o wt od ot h ef o l l o wi n ga ndwh a te a c hi su s e df or :Gr a ms t a i n ,ne g a t i v es t a i n,s t r e a kpl a t e , s pr e a dp l a t e ,‘ l a wn ’c ul t u r ef ora nt i mi c r o bi a ls us c e pt i b i l i t y . 6 .Kno wt heba s i cl a bs a f e t ypr oc e d ur e sa nda dd i t i on a ls a f e t yf orBSL2. 7 .Und e r s t a ndho wme di af ora e r ot ol e r a n c et e s t si sma dea n dho wt h a tc on t r i b ut e st oi nt e r pr e t a t i o no f t her e s ul t s . 8 .Kno wt hel o gi c a ls e q ue n c eofs t e psi ni de nt i f yi n gas pe c i ficun kn o wn( e . g , aGr a mn e g a t i v er odo ra Gr a mpo s i t i v ec oc c u s ) . 8 . Kn o wt hep a r t soft h emi c r os c op ea ndt he i ru s e s . 9 . Kn o wh o wt op e r f or m as e r i a ldi l u t i on se xp e r i me nta nddoc a l c ul a t i on s .Re me mbe r ,noc a l c ul a t or s wi l lbea l l o we do nt h efin a le x a m.Wh ya r es e r i a ld i l ut i on sne c e s s a r y ? 1 0. Kno wt hepr i n c i pl e soft r a ns f or ma t i on ,t h ep GLOs y s t e m,a ndwh yt hev a r i o usc o nt r ol sa r eu s e d. 1 1. Kno wwh a tc ol i f o r ms ,e nt e r oc oc c ie t ca r ea ndho wt ode t e c tt he m 1 2. Ha v eag e ne r a li de aa bo utmi c r oa l g a ea swe l la st hepr i n c i pl e sbe h i n dt h eFv / Fmme a s u r e me nt s .

2 1 3. Kno wt hema j ors t r u c t ur e soff u n gi ,t hedi ffe r e n c e sb e t we e nmo l d sa ndy e a s t , e t c .Ho wa r ey e a s t d i ffe r e ntt ha nb a c t e r i a ?...