Physical Lab 2Mouthwash PDF

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PHYSICAL LAB 2 - MOUTHWASH

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Physical Lab 2 - Mouthwash

Blanca Cordero Clovis Community College Microbiology 301 Ms. Michelle Hughes November 17, 2020

PHYSICAL LAB 2 - MOUTHWASH

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Physical Lab 2 - Mouthwash Title: Mouthwash lab Purpose: Examine the bacterial growth difference between two different mouthwash types. This experiment aims to investigate which brands of mouth wash are more effective in killing bacteria. This will be determined by using two different mouthwash types, which will be tested on a Petri dish, each having one trial. The degree of effectiveness of the mouthwashes will be measured by the percentage of growth on the Petri dish. It will be interesting to see what kind of bacteria is found in you taking and figure out which brand of mouthwash I should be using, especially now that I have braces. Hypothesis: Alcoholic mouthwash will allow less bacterial growth than non-alcoholic mouthwash. I hypothesize that using different mouthwashes will inhibit or kill oral bacteria found in the human oral cavity. I also think that the mouthwash with alcohol (Listerine brand) will be more effective in killing oral bacteria because alcohol solutions are used in the medical field to disinfect surfaces and kill bacteria. Therefore, the Listerine brand should be the most effective mouthwash. Introduction: Many oral bacteria contribute to diseases of the mouth. One of the most commons is Streptococcus mutants, gram-positive, anaerobic bacteria that live in the oral cavity. (Alcohol VS. Alcohol Free Mouthwash: What's the Difference?, n.d.)When it comes to mouthwash, there are two different types available for people to use: alcohol is alcoholic and non-alcoholic. Listerine is an alcoholic mouthwash, while crest is a non-alcoholic mouthwash. The active ingredient in the crest is .07% Acetyl chloride and in Listerine is menthol. Mouthwashes work by penetrating the plaque bifilar, killing bacteria. The #1 dentist recommended mouthwash is Listerine.(Cool Mint® Antiseptic Mouthwash | Listerine®, n.d.)

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Materials & Methods According to Ms.Hughes (2020) Material: Petri dish, alcoholic mouthwash, non-alcoholic mouthwash, bacterial sample, cotton swab, distilled water, pipet, test tubes, Permanent Marker, Incubator, lab coat, eyeprotective glasses, gloves, ruler, a camera, pen and paper, and your computer. Methods: According to Hughes (2020) It is important when culturing any bacteria. You must treat the bacteria as harmful to your health and use sterile equipment when collecting your bacteria to ensure you collect the bacteria properly. First: Pull hair back, gather all materials, wash hands for 30-60 seconds, apply lab coat and eye-protective glasses, Make sure area is nice and clean, and disinfecting to start a lab, wash hands once again for 30-60 seconds, and began lab. Disinfect work areas before and after use. Use a disinfectant to wipe down benches and work areas both before and after working with cultures. Wash your hands. Use a disinfectant soap to wash your hands before and after working with microorganisms. Wear gloves for extra protection. Obtain one Petri dishes, label one side A for alcoholic and the opposite side of the Petri dish, NA for non-alcoholic with a sharpie. With a sterile swab of cotton, take an oral sample and mix it into distilled water until turbid. Mix 1 ml of the mouth sample into 3 mL of the alcoholic and alcohol-free mouthwash samples. Let sit for one minute. Using another sterile cotton swab, spread the turbid mouthwash samples onto a Petri dish in a zigzag motion. Do this for both mouthwash samples.

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Keep in a warm environment and let bacteria grow for 120 hours, a total of 5 days. Disinfect work areas after use. Use a disinfectant to wipe down benches and work areas both before and after working with cultures. Wash your hands. Use a disinfectant soap to wash your hands before and after working with microorganisms. Wear gloves for extra protection after use. Properly dispose of all waste material and disinfect equipment. All items to be discarded after a class should be disposed of properly or isolated and kept away from clean equipment. To dispose of bacteria after 7 days, place in an incubator at high heat to kill bacteria. If done at home, fill the Petri dish with blench for 24 hours and then dispose of it. Results: The results I received were that the alcoholic mouthwash allowed less bacterial growth than the non-alcoholic mouthwash, which proves my hypothesis correct.

Table 1 Listerine vs Crest Listerine Alcohol number of colonies Size in (mm) of the colony Color of colonies

Alcohol 0 colonies wear found after 5days, 120 hours N/A no growth N/A- no colonies found

Crest Non- AlcoholNumber of colonies Size in (mm) of the colony Color of colonies

Non-Alcohol 1 large colony was found after 5days, 120 hours 2 mm size of the colony White in color of colony

Conclusion: Restate: examine different bacterial growth between two different types of mouthwash. Explain: using a Petri dish doing the zigzag method, a bacterial sample was used to see the growth inhibition that two different types of mouthwash allow.

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Results; The alcoholic mouth wash killed more bacteria than the non-alcoholic mouthwash; therefore, my hypothesis was correct.” The alcoholic mouthwash will allow less bacterial growth than non-alcoholic mouthwash. I hypothesize that using different mouthwashes will inhibit or kill oral bacteria found in the human oral cavity. I also think that the mouthwash with alcohol (Listerine brand) will be more effective in killing oral bacteria because alcohol solutions are used in the medical field to disinfect surfaces and kill bacteria. Therefore, the Listerine brand should be the most effective mouthwash.” Uncertainties: What were potential sources of error in your experiment? These are circumstances beyond your control that may have (or could potentially) impact the experiment. This is going to require some critical thinking. Answer: Some potential errors that could occur during this experiment could be not cleaning the equipment correctly.Opening and closing the Petri dish when not in use so the bacteria may grow wrong and give different results. Also, not doing this experiment for the full 5days 120 hours could give false results. New: How could you extend/expand on this experiment if you were to take it a step further? This also requires some critical thinking. Answer: I would incubate in a better environment to maximize bacterial growth. I would also like to extend/expand the experiment using more Alcohol and Non-alcohol mouthwashes instead of just the 2 types. I would use other ones and compare them all; also, I would like to do one person with braces and one person without braces to see the difference in bacteria growth.

PHYSICAL LAB 2 - MOUTHWASH

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Alcohol vs. alcohol free mouthwash: What's the difference? (n.d.). https://www.alliancedental.ca/oral-health-tips/alcohol-vs-alcohol-free-mouthwash-whatsthe-difference/ Anchan, A. (2013, December 15). How mouthwash can help fight germs in your mouth | thehealthsite.com. https://www.thehealthsite.com/oral-health/how-mouthwash-can-helpfight-germs-in-your-mouth-100472/ Cool mint® antiseptic mouthwash | listerine®. (n.d.). LISTERINE®. https://www.listerine.com/mouthwash/antiseptic/listerine-cool-mintmouthwash?utm_source=google&utm_medium=cpc Microorganisms safety. (2008). Science Buddies. https://www.sciencebuddies.org/science-fairprojects/references/microorganisms-safety...