Physio Ex Exercise 1 Active transport PDF

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PhysioEx Exercise 1 Activity 5

6/13/21, 8 :47 AM

PhysioEx Lab Report Exercise 1: Cell Transport Mechanisms and Permeability Activity 5: Simulating Active Transport Name: Joshua Hammond Date: 13 June 2021 Session ID: session-05527ead-e267-a8cf-a18a-b4a2f9f11078

Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 The movement of sodium and potassium maintained by the Na +-K +pump You correctly answered: requires energy and is against a concentration gradient. 2 The sodium-potassium pump is classified as a(n) You correctly answered: antiporter. 3 The sodium-potassium pump moves _____ sodium ions and _____ potassium ions simultaneously. You correctly answered: 3, 2. 4 Solutes that require active transport for movement might be too large to pass or might be You correctly answered: lipid insoluble.

Experiment Results Predict Questions 1 Predict Question 1: What do you think will result from these experimental conditions? Your answer: Na + will be maximally transported. 2 Predict Question 2: Do you think the addition of glucose carriers will affect the transport of sodium or potassium? Your answer: Yes, it will affect the transport of both ions. https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act5/

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PhysioEx Exercise 1 Activity 5

6/13/21, 8 :47 AM

Stop & Think Questions 1 Why did the sodium transport stop before the transport was completed? You correctly answered: The ATP was depleted. 2 Why was the equilibrium for the solutes reached earlier? You correctly answered: There were more pumps for transport. Experiment Data Run Number

Solute

ATP (mM)

Start Conc. L (mM)

Start Conc. R (mM)

Pumps

Carriers

Rate (mM/sec)

1

Na + Cl -

1

9.000

0.000

500

---

0.0188

1

K+ Cl-

1

0.000

6.000

500

---

0.0125

1

Glucose

---

0.000

0.000

---

0

0.0000

3

Na + Cl -

3

9.000

0.000

500

---

0.0025

3

K+ Cl-

3

0.000

6.000

500

---

0.0017

3

Glucose

---

0.000

0.000

---

0

0.0000

4

Na + Cl -

3

9.000

0.000

500

---

0.0000

4

K+ Cl-

3

0.000

0.000

500

---

0.0000

4

Glucose

---

0.000

0.000

---

0

0.0000

5

Na + Cl -

3

9.000

0.000

800

---

0.0042

5

K+ Cl-

3

0.000

6.000

800

---

0.0028

5

Glucose

---

0.000

0.000

---

0

0.0000

6

+

Na Cl

3

9.000

0.000

800

---

0.0042

6

K+ Cl-

3

0.000

6.000

800

---

0.0028

6

Glucose

---

0.000

10.000

---

400

0.0028#

-

Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 What happened when you increased the amount of ATP dispensed with the same concentration of sodium and potassium on either side of the membrane?

https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act5/

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PhysioEx Exercise 1 Activity 5

6/13/21, 8 :47 AM

You correctly answered: More ions were transported. 2 At what concentration of ATP were the sodium and potassium maximally transported? You correctly answered: 3 mM ATP. 3 What was the effect of adding more Na +-K + pumps to the simulated cell? You correctly answered: Transport of the ions was faster. 4 Describe the effect of adding glucose carriers to the sodium and potassium transport. You correctly answered: There was no change in the transport rate because glucose is transported independently.

Review Sheet Results 1 Describe the significance of using 9 mM sodium chloride inside the cell and 6 mM potassium chloride outside the cell, instead of other concentration ratios. Your answer: The significance of using the 9mM sodium chloride inside the cell and 6 mM potassium chloride outside the cell is because for every 3 Na+ ions that cells, the cell 2 Ka+ ions enter. 2 Explain why there was no sodium transport even though ATP was present. How well did the results compare with your prediction? Your answer: There was no sodium transport even though ATP was present is due to more sodium being outside the cell and only is transported outside if needed. My prediction that Na+ would be maximally transported was incorrect because there was no need for it to moved. 3 Explain why the addition of glucose carriers had no effect on sodium or potassium transport. How well did the results compare with your prediction? Your answer: The addition of glucose carriers did not affect the sodium and potassium transport https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act5/

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PhysioEx Exercise 1 Activity 5

6/13/21, 8 :47 AM

because glucose is transported typically through facilitated diffusion. My prediction was that it would affect the transport of both ions, but that was incorrect. 4 Do you think glucose is being actively transported or transported by facilitated diffusion in this experiment? Explain your answer. Your answer: Glucose was being actively transported during this experiment because ATP was present.

https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act5/

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