Physio Ex Exercise 1 Activity 1 PDF

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9/18/2020

PhysioEx Exercise 1 Activity 1

PhysioEx Lab Report Exercise 1: Cell Transport Mechanisms and Permeability Activity 1: Simulating Dialysis (Simple Diffusion) Name: Kelly Isidro Date: 18 September 2020 Session ID: session-4604ab7f-3c43-aee5-d7e9-4191050adc35

Pre-lab Quiz Results You scored 100% by answering 3 out of 3 questions correctly. 1 The driving force for diffusion is You correctly answered: the kinetic energy of the molecules in motion. 2 In diffusion, molecules move You correctly answered: from high concentration to low concentration. 3 Which of the following dialysis membranes has the largest pore size? You correctly answered: 200 MWCO.

Experiment Results Predict Questions 1 Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane? Your answer: Yes, but very slowly. 2 Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607 amino acids, and the average molecular weight of a single amino acid is 135 g/mol. Which of the following will be able to diffuse through the 200 MWCO membrane? Your answer: glucose only. Stop & Think Questions 1 The reason sodium chloride didn't diffuse left to right is that You correctly answered: the membrane pore size was too small. https://media.pearsoncmg.com/bc/bc 0media ap/physioex/10/ex1/act1/

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PhysioEx Exercise 1 Activity 1

2 Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average molecular weight of a single amino acid is 135 g/mol. There is no reason to run these solutes at the 20 MWCO because You correctly answered: glucose and albumin are both too large to pass. 3 The rate of diffusion for urea You correctly answered: is slower than that for sodium because urea is a larger molecule. Experiment Data Solute

MWCO

Left Solute (mM)

Right Solute (mM)

Average Diffusion (mM/sec)

Na + Cl -

20

9.00

0.00

0.0000

Urea

20

0.00

0.00

0.0000

Albumin

20

0.00

0.00

0.0000

Glucose

20

0.00

0.00

0.0000

Na + Cl -

20

0.00

0.00

0.0000

Urea

20

9.00

0.00

0.0000

Albumin

20

0.00

0.00

0.0000

Glucose

20

0.00

0.00

0.0000

Na + Cl -

50

9.00

0.00

0.0150

Urea

50

0.00

0.00

0.0000

Albumin

50

0.00

0.00

0.0000

Glucose

50

0.00

0.00

0.0000

Na + Cl -

50

18.00

0.00

0.0273

Urea

50

0.00

0.00

0.0000

Albumin

50

0.00

0.00

0.0000

Glucose

50

0.00

0.00

0.0000

Na + Cl -

100

9.00

0.00

0.0150

Urea

100

0.00

0.00

0.0000

Albumin

100

0.00

0.00

0.0000

Glucose

100

0.00

0.00

0.0000

Na + Cl -

100

0.00

0.00

0.0000

Urea

100

9.00

0.00

0.0094

Albumin

100

0.00

0.00

0.0000

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PhysioEx Exercise 1 Activity 1

Glucose

100

0.00

0.00

0.0000

Na + Cl -

200

0.00

0.00

0.0000

Urea

200

0.00

0.00

0.0000

Albumin

200

0.00

0.00

0.0000

Glucose

200

9.00

0.00

0.0042

Na + Cl -

200

0.00

0.00

0.0000

Urea

200

0.00

0.00

0.0000

Albumin

200

9.00

0.00

0.0000

Glucose

200

0.00

0.00

0.0000

Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 The effect of increasing the concentration of sodium chloride from 9 mM to 18 mM in the left beaker was to You correctly answered: increase the rate of diffusion. 2 Describe the difference between the rate of diffusion seen for sodium and urea. You correctly answered: Urea diffused more slowly because it is larger than sodium. 3 Which of the following solutes did not pass through any of the membranes? You correctly answered: albumin. 4 When diffusion stops, we say the solution has reached You correctly answered: equilibrium.

Review Sheet Results 1 Describe two variables that affect the rate of diffusion. Your answer: 2 variables that affect the rate of diffusion are the Molecular Weight, and Solute Concentration. 2 Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? https://media.pearsoncmg.com/bc/bc 0media ap/physioex/10/ex1/act1/

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Your answer: The lower the MWCO, the smaller the pores in the membrane are and Urea has a weight of 60.07 which is obviously bigger than 20. Therefore, being too big to pass through easily. 3 Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? Your answer: My predictions were right, glucose was able to pass because it is smaller and Albumin being a protein with a high molecular weight was not able to diffuse. 4 Put the following in order from smallest to largest molecular weight: glucose, sodium chloride, albumin, and urea. Your answer: Sodium Chloride, Urea, Glucose, ,Albumin.

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