Physio Ex Exercise 1 Activity 5 PDF

Preview

Summary

Pearson PhysioEx Lab ...

Description

PhysioEx Lab Report Exercise 1: Cell Transport Mechanisms and Permeability Activity 5: Simulating Active Transport Name: Kaley Faucher Date: 15 September 2020 Session ID: session-30f35ffc-b515-b23f-f3f6-822d94d74f67

Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 The movement of sodium and potassium maintained by the Na +-K +pump You correctly answered: requires energy and is against a concentration gradient. 2 The sodium-potassium pump is classified as a(n) You correctly answered: antiporter. 3 The sodium-potassium pump moves _____ sodium ions and _____ potassium ions simultaneously. You correctly answered: 3, 2. 4 Solutes that require active transport for movement might be too large to pass or might be You correctly answered: lipid insoluble.

Experiment Results Predict Questions 1 Predict Question 1: What do you think will result from these experimental conditions? Your answer: Na + will be maximally transported. 2 Predict Question 2: Do you think the addition of glucose carriers will affect the transport of sodium or potassium? Your answer: No, it will not affect the transport of either ion. Stop & Think Questions 1 Why did the sodium transport stop before the transport was completed? You correctly answered: The ATP was depleted.

2 Why was the equilibrium for the solutes reached earlier? You correctly answered: There were more pumps for transport. Experiment Data Run Number

Solute

ATP (mM)

Start Conc. L (mM)

Start Conc. R (mM)

Pumps

Carriers

Rate (mM/sec)

1

Na+ Cl-

1

9.000

0.000

500

---

0.0188

1

0.000

6.000

500

---

0.0125

+

-

1

K Cl

1

Glucose

---

0.000

0.000

---

0

0.0000

2

Na+ Cl-

3

9.000

0.000

500

---

0.0025

2

K+ Cl -

3

0.000

6.000

500

---

0.0017

2

Glucose

---

0.000

0.000

---

0

0.0000

3

+

Na Cl

3

9.000

0.000

500

---

0.0000

3

K+ Cl -

3

0.000

0.000

500

---

0.0000

3

Glucose

---

0.000

0.000

---

0

0.0000

4

+

3

9.000

0.000

800

---

0.0042

3

0.000

6.000

800

---

0.0028

Na Cl +

-

-

-

4

K Cl

4

Glucose

---

0.000

0.000

---

0

0.0000

5

Na+ Cl-

3

9.000

0.000

800

---

0.0042

3

0.000

6.000

800

---

0.0028

---

0.000

10.000

---

400

0.0028#

+

-

5

K Cl

5

Glucose

Post-lab Quiz Results You scored 50% by answering 2 out of 4 questions correctly. 1 What happened when you increased the amount of ATP dispensed with the same concentration of sodium and potassium on either side of the membrane? Your answer: The ions were transported more slowly. Correct answer: More ions were transported. 2 At what concentration of ATP were the sodium and potassium maximally transported? Your answer: 1 mM ATP. Correct answer: 3 mM ATP. 3 What was the effect of adding more Na+ -K+ pumps to the simulated cell?

You correctly answered: Transport of the ions was faster. 4 Describe the effect of adding glucose carriers to the sodium and potassium transport. You correctly answered: There was no change in the transport rate because glucose is transported independently.

Review Sheet Results 1 Describe the significance of using 9 mM sodium chloride inside the cell and 6 mM potassium chloride outside the cell, instead of other concentration ratios. Your answer: Knowing that Na/K+ pumps work with a 3:2 ratio, it is ideal to use 9mM:6mM for a concentration ratio. 2 Explain why there was no sodium transport even though ATP was present. How well did the results compare with your prediction? Your answer: Sodium by itself will not make the pump work. Sodium and potassium are needed for Na/K+ pump. 3 Explain why the addition of glucose carriers had no effect on sodium or potassium transport. How well did the results compare with your prediction? Your answer: Since glucose works independently and is NOT necessary, it does not help with sodium transport. 4 Do you think glucose is being actively transported or transported by facilitated diffusion in this experiment? Explain your answer. Your answer: It is transported by active transport in this experiment, due to the amount of ATP dispursed. A carrier is needed to transport glucose across the cell membrane due to it's size, it is too large, and lipid insoluble also....