Physio Ex Exercise 1 Activity 5 PDF

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Physio Ex Lab Notes for Exercise 1 Activity 5. Expand more on the written questions to get a higher mark. ...

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5/17/2020

PhysioEx Exercise 1 Activity 5

PhysioEx Lab Report Exercise 1: Cell Transport Mechanisms and Permeability Activity 5: Simulating Active Transport Name: Date: 17 May 2020 Session ID:

Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 The movement of sodium and potassium maintained by the Na+-K+ pump You correctly answered: requires energy and is against a concentration gradient. 2 The sodium-potassium pump is classified as a(n) You correctly answered: antiporter. 3 The sodium-potassium pump moves _____ sodium ions and _____ potassium ions simultaneously. You correctly answered: 3, 2. 4 Solutes that require active transport for movement might be too large to pass or might be You correctly answered: lipid insoluble.

Experiment Results Predict Questions 1 Predict Question 1: What do you think will result from these experimental conditions? Your answer: No Na + will be transported. 2 Predict Question 2: Do you think the addition of glucose carriers will affect the transport of sodium or potassium? Your answer: No, it will not affect the transport of either ion. Stop & Think Questions 1 Why did the sodium transport stop before the transport was completed? You correctly answered: The ATP was depleted. 2 Why was the equilibrium for the solutes reached earlier? You correctly answered: There were more pumps for transport. https://media.pearsoncmg.com/bc/bc 0media ap/physioex/10/ex1/act5/

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5/17/2020

PhysioEx Exercise 1 Activity 5

Experiment Data Run Number

Solute

ATP (mM)

Start Conc. L (mM)

Start Conc. R (mM)

Pumps

Carriers

Rate (mM/sec)

1

Na+ Cl-

1

9.000

0.000

500

---

0.0188

1

0.000

6.000

500

---

0.0125

+

-

1

K Cl

1

Glucose

---

0.000

0.000

---

0

0.0000

2

+

Na Cl

3

9.000

0.000

500

---

0.0025

2

K+ Cl-

3

0.000

6.000

500

---

0.0017

2

Glucose

---

0.000

0.000

---

0

0.0000

3

Na+ Cl-

3

9.000

0.000

500

---

0.0000

+

-

-

3

K Cl

3

0.000

0.000

500

---

0.0000

3

Glucose

---

0.000

0.000

---

0

0.0000

4

Na+ Cl-

3

9.000

0.000

800

---

0.0042

3

0.000

6.000

800

---

0.0028

+

-

4

K Cl

4

Glucose

---

0.000

0.000

---

0

0.0000

5

+

3

9.000

0.000

800

---

0.0042

-

Na Cl +

-

5

K Cl

3

0.000

6.000

800

---

0.0028

5

Glucose

---

0.000

10.000

---

400

0.0028#

Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 What happened when you increased the amount of ATP dispensed with the same concentration of sodium and potassium on either side of the membrane? You correctly answered: More ions were transported. 2 At what concentration of ATP were the sodium and potassium maximally transported? You correctly answered: 3 mM ATP. 3 What was the effect of adding more Na+-K+ pumps to the simulated cell? You correctly answered: Transport of the ions was faster. 4 Describe the effect of adding glucose carriers to the sodium and potassium transport. You correctly answered: There was no change in the transport rate because glucose is transported independently.

Review Sheet Results https://media.pearsoncmg.com/bc/bc 0media ap/physioex/10/ex1/act5/

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PhysioEx Exercise 1 Activity 5

1 Describe the significance of using 9 mM sodium chloride inside the cell and 6 mM potassium chloride outside the cell, instead of other concentration ratios. Your answer: This is very significant because this is like the sodium-potassium pump that works in our body. The sodium-potassium pump has the ratio of 3 sodium ions that leaves the cell and 2 potassium ions that are transported in. For this experiment, it uses the ratios of 9:6, which is basically the same as 3:2 in fractions. 2 Explain why there was no sodium transport even though ATP was present. How well did the results compare with your prediction? Your answer: In order for the sodium-potassium pump to work, both ions of sodium and potassium must be present. Sodium alone cannot do any exchange by itself to transport. 3 Explain why the addition of glucose carriers had no effect on sodium or potassium transport. How well did the results compare with your prediction? Your answer: My prediction was correct that it will not affect the transport of either ion because glucose transport works independently with glucose transporter proteins. 4 Do you think glucose is being actively transported or transported by facilitated diffusion in this experiment? Explain your answer. Your answer: I think glucose is being transported by facilitated diffusion in this experiment. It is going with the concentration gradient and due to its size, it is too big to pass through the cell membrane. Therefore, it requires a glucose transporter to cross the membrane.

https://media.pearsoncmg.com/bc/bc 0media ap/physioex/10/ex1/act5/

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