Physio Ex Exercise 1 Activity 5 PDF

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9/8/2020

PhysioEx Exercise 1 Activity 5

PhysioEx Lab Report Exercise 1: Cell Transport Mechanisms and Permeability Activity 5: Simulating Active Transport Name: Shan Noorani Date: 8 September 2020 Session ID: session-fc3b437f-5c21-88c5-491a-0acddd1f27a8

Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 The movement of sodium and potassium maintained by the Na +-K +pump You correctly answered: requires energy and is against a concentration gradient. 2 The sodium-potassium pump is classified as a(n) You correctly answered: antiporter. 3 The sodium-potassium pump moves _____ sodium ions and _____ potassium ions simultaneously. You correctly answered: 3, 2. 4 Solutes that require active transport for movement might be too large to pass or might be You correctly answered: lipid insoluble.

Experiment Results Predict Questions 1 Predict Question 1: What do you think will result from these experimental conditions? Your answer: No Na + will be transported. 2 Predict Question 2: Do you think the addition of glucose carriers will affect the transport of sodium or potassium? Your answer: No, it will not affect the transport of either ion. Stop & Think Questions 1 Why did the sodium transport stop before the transport was completed? You correctly answered: The ATP was depleted.

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PhysioEx Exercise 1 Activity 5

2 Why was the equilibrium for the solutes reached earlier? You correctly answered: There were more pumps for transport. Experiment Data Run Number

Solute

ATP (mM)

Start Conc. L (mM)

Start Conc. R (mM)

Pumps

Carriers

Rate (mM/sec)

1

Na+ Cl-

1

9.000

0.000

500

---

0.0188

1

0.000

6.000

500

---

0.0125

+

-

1

K Cl

1

Glucose

---

0.000

0.000

---

0

0.0000

2

Na+ Cl-

3

9.000

0.000

500

---

0.0025

2

K+ Cl -

3

0.000

6.000

500

---

0.0017

2

Glucose

---

0.000

0.000

---

0

0.0000

3

+

Na Cl

3

9.000

0.000

500

---

0.0000

3

K+ Cl -

3

0.000

0.000

500

---

0.0000

3

Glucose

---

0.000

0.000

---

0

0.0000

4

+

3

9.000

0.000

800

---

0.0042

3

0.000

6.000

800

---

0.0028

Na Cl +

-

-

-

4

K Cl

4

Glucose

---

0.000

0.000

---

0

0.0000

5

Na+ Cl-

3

9.000

0.000

800

---

0.0042

3

0.000

6.000

800

---

0.0028

---

0.000

10.000

---

400

0.0028#

+

-

5

K Cl

5

Glucose

Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 What happened when you increased the amount of ATP dispensed with the same concentration of sodium and potassium on either side of the membrane? You correctly answered: More ions were transported. 2 At what concentration of ATP were the sodium and potassium maximally transported? You correctly answered: 3 mM ATP. 3 What was the effect of adding more Na +-K + pumps to the simulated cell? You correctly answered: Transport of the ions was faster. 4 Describe the effect of adding glucose carriers to the sodium and potassium transport. https://media.pearsoncmg.com/bc/bc 0media ap/physioex/10/ex1/act5/

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PhysioEx Exercise 1 Activity 5

You correctly answered: There was no change in the transport rate because glucose is transported independently.

Review Sheet Results 1 Describe the significance of using 9 mM sodium chloride inside the cell and 6 mM potassium chloride outside the cell, instead of other concentration ratios. Your answer: The significance of using 9 mM sodium chloride inside the cell and 6 mM potassium chloride outside the the cell is that the Na/K pump allows for a 3:2 ratio. For every three sodium ions that go out of the cell, two potassium ions come into the cell. 2 Explain why there was no sodium transport even though ATP was present. How well did the results compare with your prediction? Your answer: Both sodium and potassium are required to move the ions using the pump. If one of the two are absent then no movement will occur. Potassium needs to be present in order for for sodium to be transported. 3 Explain why the addition of glucose carriers had no effect on sodium or potassium transport. How well did the results compare with your prediction? Your answer: This is because the glucose carriers behave independently from the sodium and potassium pumps. The results compared well with the prediction because the rates of the sodium and potassium pumps were the same before and after the addition of the glucose carriers. 4 Do you think glucose is being actively transported or transported by facilitated diffusion in this experiment? Explain your answer. Your answer: Glucose is being transported by facilitated diffusion because the actual process of glucose transport is done without ATP and needs only a transport protein. Glucose transport is coupled with the sodium potassium pump transport of ions to create the gradient that powers the transportation of glucose.

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