Physio Ex Exercise 1 Activity 1 PDF

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5/12/2020

PhysioEx Exercise 1 Activity 1

PhysioEx Lab Report Exercise 1: Cell Transport Mechanisms and Permeability Activity 1: Simulating Dialysis (Simple Diffusion) Name: Cheigh Date: 12 May 2020 Session ID: session-7e211c19-38cd-3bd1-011b-192c3fe04fbb

Pre-lab Quiz Results You scored 100% by answering 3 out of 3 questions correctly. 1 The driving force for diffusion is You correctly answered: the kinetic energy of the molecules in motion. 2 In diffusion, molecules move You correctly answered: from high concentration to low concentration. 3 Which of the following dialysis membranes has the largest pore size? You correctly answered: 200 MWCO.

Experiment Results Predict Questions 1 Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane? Your answer: No, not at all. 2 Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607 amino acids, and the average molecular weight of a single amino acid is 135 g/mol. Which of the following will be able to diffuse through the 200 MWCO membrane? Your answer: glucose only. Stop & Think Questions 1 The reason sodium chloride didn't diffuse left to right is that You correctly answered: the membrane pore size was too small.

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PhysioEx Exercise 1 Activity 1

2 Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average molecular weight of a single amino acid is 135 g/mol. There is no reason to run these solutes at the 20 MWCO because You correctly answered: glucose and albumin are both too large to pass. 3 The rate of diffusion for urea You correctly answered: is slower than that for sodium because urea is a larger molecule. Experiment Data Solute

MWCO

Left Solute (mM)

Right Solute (mM)

Average Diffusion (mM/sec)

Na+ Cl -

20

9.00

0.00

0.0000

Urea

20

0.00

0.00

0.0000

Albumin

20

0.00

0.00

0.0000

Glucose

20

0.00

0.00

0.0000

20

0.00

0.00

0.0000

Urea

20

9.00

0.00

0.0000

Albumin

20

0.00

0.00

0.0000

Glucose

20

0.00

0.00

0.0000

50

9.00

0.00

0.0150

Urea

50

0.00

0.00

0.0000

Albumin

50

0.00

0.00

0.0000

Glucose

50

0.00

0.00

0.0000

50

18.00

0.00

0.0273

Urea

50

0.00

0.00

0.0000

Albumin

50

0.00

0.00

0.0000

Glucose

50

0.00

0.00

0.0000

+

100

9.00

0.00

0.0150

Urea

100

0.00

0.00

0.0000

Albumin

100

0.00

0.00

0.0000

Glucose

100

0.00

0.00

0.0000

+

100

0.00

0.00

0.0000

Urea

100

9.00

0.00

0.0094

Albumin

100

0.00

0.00

0.0000

Glucose

100

0.00

0.00

0.0000

+

200

0.00

0.00

0.0000

Na+

Na+

Na+

Cl -

Cl -

Cl -

Na Cl

Na Cl

Na Cl

-

-

-

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PhysioEx Exercise 1 Activity 1

Urea

200

0.00

0.00

0.0000

Albumin

200

9.00

0.00

0.0000

Glucose

200

0.00

0.00

0.0000

Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 The effect of increasing the concentration of sodium chloride from 9 mM to 18 mM in the left beaker was to You correctly answered: increase the rate of diffusion. 2 Describe the difference between the rate of diffusion seen for sodium and urea. You correctly answered: Urea diffused more slowly because it is larger than sodium. 3 Which of the following solutes did not pass through any of the membranes? You correctly answered: albumin. 4 When diffusion stops, we say the solution has reached You correctly answered: equilibrium.

Review Sheet Results 1 Describe two variables that affect the rate of diffusion. Your answer: Pore size of the membrane- can allow for more solute and bigger solute to pass Solute concentration - the more solute and the higher the concentration gradient, the faster solute will move. 2 Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? Your answer: My prediction was correct, urea didn't diffuse due to its size. 3 Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? Your answer:

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This was true, since glucose was small enough it diffused, but albumin did not diffuse at all, likely due to its size. 4 Put the following in order from smallest to largest molecular weight: glucose, sodium chloride, albumin, and urea. Your answer: Sodium chloride, Urea, Glucose, Albumin.

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