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Summary

Microbiology and Parasitology Review Study online at 1. AFB grading fields 2. Optochin for S. pneumoniae 3. 0 AFB grading No fields 4. 0 microns HEPA Filter (size) 5. 0 units Concentration of bacitracin 6. AFB grading fields 7. 1:5 Ratio of blood to broth in blood culture 8. AFB grading in at least ...

Description

Microbiology and Parasitology Review Study online at quizlet.com/_2dzhdx 1.

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AFB grading 1-9 AFB/100 fields

2.

>14mm

Optochin for S. pneumoniae

3.

0

AFB grading No AFB/300 fields

4.

0.03 microns

HEPA Filter (size)

5.

0.04 units

Concentration of bacitracin

6.

1+

AFB grading 10-99 AFB/100 fields

7.

1:5

Ratio of blood to broth in blood culture

8.

2+

AFB grading 1-10 AFB/ in at least 50 fields

9.

2-4% NaOH

Decontaminate mucis

10.

2x3 cm

Ideal size of AFB smear

11.

3+

AFB grading >10 AFB/ in at least 20 fields

12.

4'C

Transport temperature virus and sputume

13.

5-10% CO2

Requirement for Capnophiles

14.

5ug

Concentration of Optochin

15.

7.2-7.4

pH of culture media to bacteria

16.

7.5 % Mannitol Salt Agar Selective media of Staphylococcus aureus

17.

10-30 min

Sodium Hypochlorite (time to disinfect)

18.

30% H2O2

Superoxol Catalase Test reagent

19.

37 degrees

CSF storage temp

20.

(-70 degrees Celsius)

Transport virus specimen for days (temp)

21.

75k-15k count

Before and after pasteurisation (count)

22.

Abiotrophia spp Granulicatella

Nutritionally variant streptococci (2) Requires Vitamin B6 (pyridoxine)

23.

Actinobacillus Star like colony actinomycetemcomitans

24.

Actinobacter

#2 NFO

25.

Actinomyces

Ray fungus

26.

Actinymyces

Fungus like bacteria, Anerobic, Gram pos

27.

Aeromonas hyrophila

Causative agent of red leg disease

28.

Alkalescens dispar

Only E.coli that is non lactose fermenter and non motile

29.

Alpha prime hemolysis

Small zone of alpha hemolysis surrounded by zone of beta hemolysis after refrigeration

30.

Ammonia

By product of acetamide utilization

31.

Ammonia

Acetamide by product

32.

Anaerobic atmosphere

5% CO2, 10% H2, 85% N2

33.

Aphtovirus

Foot, mouth disease virus

34.

Arcanobacterium haemolyticum

Beta hemolysis on SBAP Lipase: pos Lecithinase: pos Reverse CAMP: pos

35.

Arizona spp

Related to salmonella TSI: A/@ H2S ONPG: (+) (only salmonella that is lactose fermenter)

36.

Arylsulfatase test

Test for Rapid growers of Mycbacteria

37.

Ascoli test

Animal test for anthrax Ag

38.

Aspergillus fumigatus Mycetoma and Black Lung

39.

Asteroid body

40.

Atrhobacter

Brevibacterium

41.

Autoclave

121'C 15lbs 15-20 min

42.

Babes-Ernst

Metachromatic granules of Corynebacteria

43.

Bacillus

Gram pos bacilli Motility: (+) Esculin HOH: (+) Salicin: (+) CAMP: (+)

44.

Bacillus anthracis

Mx: Gram pos rods in chains, bamboo pole appearance Nonmotile BAP: nonhemolytic, irregular, serrated, swirling Penicillin Sensitive

45.

Bacillus anthracis

Mx: Gram pos bacilli in chains Motility: neg Capsule: pos Hemolysis: neg Growth 45°C: neg Salicin ferment: neg Penicillin G: S Gelatin HOH and PEA: neg

46.

Bacillus anthracis

Largest pathogenic bacteria

47.

Bacillus anthracis

Fried Rice bacillus

48.

Bacillus cereus

Mx: Gram pos bacilli in chains Motility: pos Capsule: neg Hemolysis: beta hem Growth 45°C: pos Salicin ferment: pos Penicillin G: R Gelatin HOH and PEA: pos

Product of Ag-Ab rnx

49.

Bacillus Flat sour spoilage stearothermophilus

50.

Bacillus Quality Control for autoclave stearothermophilus

51.

Bacillus subtilis

Quality Control for incineration

52.

Bacteria

Orange fluorescence in acridine orange

53.

Bacteriodes ureolyticus

Anaerobic bacteria, pitting of agar

54.

Bacteroides

Normal flora of intestines (90%)

55.

Bartonella bacilliformis

Oroya fever Infects RBCs

56.

Bartonella henselae

Catscratch disease

57.

Before height of fever

Blood collection time

58.

Beifanti

Biotype of C. diptheriae CT-BAP: non toxigenic

59.

Bench marking

60. 61.

78.

Burkholderia cepacia

An important cause of onion bulb rot in plants and foot rot in humans. Also causes severe pneumonia in patients with cystic fibrosis

79.

Burkholderia mallei

Glander's disease Onlt non motile pseudomonad

80.

Burkholderia pseudomallei

Glanders LIKE pneumonia/Merioidosis Wrinkled colony on ashdown medium Lactose oxidizer

81.

Burkholderia pseudomallei

Vietnamese time bomb

82.

Campylobacter

Curved bacteria with darting motility

83.

Campylobacter jejuni

Hippurate hyrolysis

84.

Campylobacter jejuni Chlamydia pneumoniae

Agent of Guillain-Bare syndrome (2)

Comparing results with peers

85.

Capnocytophagia

Periodontal, gliding

Beta lactamase

Gives drug resistance to bacteria

86.

Capsule

Bile Esculin hydrolysis

Presumptive ID for Group D streptococci

Virulence factor of Streptococcus pneumoniae

87.

Bile salts and brilliant green

Inhibitors contained in SalmonellaShigella agar

Capsule Endotoxin

Virulence factor of Neisseria meningitidis (2)

88.

Catalase Test

Biosafety Cabinet Class II

exhausted and recirculated air

Test to differentiate Staphylococcus from Streptococcus Species

89.

Cetrimide

Bismuth Sulfite Agar

Selective agar for Salmonella typhi

Selective media for Pseudomonas aeroginosa

90.

Cetrimide Agar

65.

Blastomyces

Cross reacts with Histoplasmin

Selective media for Pseudomonas aeruginosa

66.

Blood

Specimen to isolate S. typhi during 1st week of infection

91.

Chain of infection

source, transmission, host

92.

Chigger

O. tsutsugamushi (Proteus Ox-K)

62.

63.

64.

67.

Blue

Citrate, Acetamide, Acetate, Malonate Pos rxn

93.

Chlamydia pneumoniae

Human to human pneumonia

68.

Blue Color

Positive reaction for Citrate and Acetate

94.

Chlamydia psittaci

Parrot fever

95.

Chocolate Agar Martin Lewis Agar

Most Appropriate primary media for endocervical specimens (2)

96.

CHO fermentation

Definitive test for Neisseria spp

97.

Chromobacterium violecium

Violet Colony

98.

Citrate

Causes false positive for Coagulase test

99.

Citrobacter

Citrate: Pos LDC: Neg Related to salmonella TSI: A/@ H2S +/ONPG: +

100.

Citrobacter freundii

Cross reacts with Salmonella typhi A/@ H2S

101.

Clammatobacterium Granuloma inguinale

102.

Clear Zone

69.

Bone marrow

Specimen that is considered to be the most sensitive for the recovery of Brucella and typhoid bacillus in cases of chronic infection

70.

Borrelia burgdorfei Bull's eye rash

71.

Borrelia burgdorferi

Lyme Disease

72.

Borrelia Fusobacterium

Vincent's angina (2)

73.

Branhamella

Old name of Moraxella spp

74.

Brilliant Green Agar

Media for other salmonella species

75.

Brucella

Bang's disease

76.

Brucella melitensis

Brucella organisms that will grow in the presence of basig fuchsin and thionine

77.

Brucella pertusis

Whooping cough

Dnase pos Methyl green

103.

Clear Zone

Dnase pos 1N HCL

104.

Clostridium botulinum

Cause of floppy baby

105.

Clostridium botulinum

Clostridium spp Motility: pos Lecithinase: neg Lipase: pos Lactose: neg Glucose: pos

106.

Clostridium botulinum

Canned good bacillus

107.

Clostridium botulinum

Block release of acetylcholine Flaccid paralysis (agent)

122.

Corynebacteria

Mx: Pleomorphic Gram pos rods, club shape, Chinese letters, palisade Catalase: pos Nonmotile Babes Ersnt (Genera)

123.

Corynebacterium

Gram pos bacilli Motility: (-) Esculin HOH: (-) Salicin: (-) CAMP: (-)

124.

Corynebacterium

Kleb Loeffler's Bacillus

125.

Corynebacterium diptheriae

Mx: Pleomorphic Gram pos rods, club shape, Chinese letters, palisade Catalase: pos Nonmotile Dextrose: (+) Maltose: (+) Dnase: pos Urease: NEG

126.

Corynebacterium minutissimum

Agent of erythrasma, coral red flouresence on woods light, presence of porphyrin

108.

Clostridium difficile

Antibiotic associated pseudomembranous enterocolitis

109.

Clostridium dificile

Clostridium spp Motility: pos Lecithinase: neg Lipase: neg Lactose: neg Glucose: pos

110.

Clostridium dificille

Causes pseudomembranous colitis in the intestine

111.

Clostridium perfringes

Encapsulated, non motile, Double zone of hemolysis

127.

Corynebacterium Hoffman's bacillus pseudoditheriticum

112.

Clostridium perfringes

Clostridium spp Motility: neg Lecithinase: pos Lipase: neg Lactose: pos Glucose: pos

128.

Cotton blue

Stain for Aman method

129.

Cotton blue

Aman medium stain

130.

Coxsakie A Virus

Foot, mouth, hand disease virus

131.

Crystal violet

Anti fungal, anti gram pos in Mac

132.

Cystine Tellurite Blood Agar media

Selective Media for Corynebacterium diptheriae

133.

Cytoplasm

Replication of Poxvirus occurs in

113.

Clostridium perfringes

Boxcar shaped bacillus

114.

Clostridium tetani

Lockjaw

115.

Clostridium tetani

Binds to ganlgioside receptors & inhibit neuron in CNS Spastic paralysis (agent)

134.

Czapeks

Aspergillus medium

135.

Czapeks Agar

Aspergillus medium

136.

Dark purple

Positive oxidase test

116.

Clostridium tetani

Tack head

137.

Decolorization

Differentiation in Gram stain

117.

Clostridium tetani

Clostridium spp Motility: pos Lecithinase: neg Lipase: neg Lactose: neg Glucose: neg

138.

Deerfly

Tularemia insect bite

139.

Dermatophytoses

Skin, hair, nail fungal infection

140.

Dick's test

Skin test for scarlet fever

141.

Differential Media

A medium that aids in the presumptive identification of organisms based on their appearance on the medium

142.

Differential Medium

Presumptive ID of organism

143.

Directly Observed Treatment Strategy

DOTS

144.

Dnase pos

Differentiates Moraxella catarrhalis from other spp

145.

E. coli

XLD yellow

146.

Ehrlichia

Sennetsu fever

Colorless colonies with black centers

Salmonella in SS Agar

Cord factor Sulfatides

Virulence factor of MTB (2)

120.

Corn Meal Agar

Media to detect chlamydospore

121.

Cornmeal agar

Media that stimulates production of chlamydospores

118.

119.

147.

Ehrlichia

Infects WBCs Murulae body

168.

Francisella tularensis

Requires Cysteine BSC III

148.

Eikenella corrodens

Human Bite wound "clenched fist"

169.

Francis test (skin test)

Serologic test for pneumococal antibody

149.

Elek test

Definitive test of Corynebacterium diptheriae, detects diptheria toxin in vitro

170.

Fungi

Green fluorescence in acridine orage

171.

Fusobacterium necrophorum

Anaerobic Mx: Gram neg bacilli Colony: Oplascent with speckles when viewd under stereoscope

172.

Gardenela vaginallis

Clue cells are found

150.

Elizabethkingjae

New name of Chryseobacterium

151.

Elizabethkingjae meingosepticum

Found in nebulizer, incubator, sink

Enteroaggregative E. coli

E. coli biotype: Stacked pattern of cells, aggregative adhesion fimbriae

173.

Gas + growth

Completed water bacteriology test

174.

TSI: A/@ IMViC: -,-,+,+ Urease: neg UTI, wound, septicemia

Gas Liquid Chromatography

Definitive test for anaerobes

Enterobacter

175.

Gastroenterities

Intestinal anthrax

176.

GC Agar

AST media for Neisseria gonorrhoea

Urease positive Enterobacter

177.

Gentamicin Blood Agar

Culture media for Streptococcus pneumoniae

152.

153.

154.

Enterobacter gergoviae

155.

Enterohemorrhagic E. coli biotype: E. coli Verotoxin, hemolytic uremic syndrome, shigella like toxin; O157:H7`

156.

Enteroinvasive E. coli

E. coli biotype: Shigella (dysentery) like toxin; O124, O143, O164

157.

Enteropathogenic E. coli

E. coli biotype: infantile diarrhea (pathogenicity island); O111

158.

Enterotoxigenic E. Coli (ETEC)

E. coli biotype: Traveller's diarrhea, cholera like toxin, heat labile enterotoxin, watery diarrhea; O6:O8:O25

178.

Germ Tube

Candida albicans in serum yields

179.

Gimenez Macchiavelo

Rickettsia stain (2)

180.

Granada medium

Selective medium for Streptococcus agalactiae

181.

Gravis

Biotype of C. diptheriae CT-BAP: gray, large, non hemolytic, starch/glycogen fermentation (+)

182.

Green

Citrate, Acetamide, Acetate, Malonate Neg rxn

183.

Green/yellow

Malonate negative

184.

Group A

Mx: cocci in chains Catalase: (-) Colony: pinpoint Bacitracin: (S) PYR: (+) SXT: (R)

159.

Enterovirus

Smallest RNA Virus (Specific)

160.

EPEC (O111)

Strain of E. coli that causes diarrheal outbreaks in infants

161.

Erysipelothrix

Gram pos bacilli H2S pos

185.

Escerichia coli

#1 cause of UTI #2 cause of neonatal meningitis

Group A, B, C, F, G

Beta hemolytic streptococcus

162.

186.

Group A Group D Enterococci

PYR + (2)

187.

Group B

Mx: cocci in chains Catalase: (-) Colony: pinpoint CAMP: (+) Hippurate HOH: (+) SXT: (R)

188.

Group B Group D Enterococci

Hippurate Hydrolysis (+) (2)

163.

Escherichia coli

TSI: A/@ IMViC: +,+,-,LOA: +,+,Greenish metallic sheen on EMB MUG: +

164.

Fitz-Hugh Curtis

Penicillinase producing Neisseria gonorrhoea

165.

Flavobacterium

Old name of Chryseobacterium

166.

Fluorescent antibody staining

Most rapid method of detecting Francisella tularensis

167.

Fluorescent microscope

Alternate of dark field microscope

214.

-Lancefield test -Fluorescent antibody test -phadebact agglutination test -neufeld-quelling

Confirmatory test for streptococcus (4)

Streptococcus Bile esculin hydrolysis (+)

215.

Lancefiled classification

Streptococcus classification based on carbohydrate on cell wall

Haemophilus

Horse Blood Agar (organism found)

216.

Legionella

BCYE, airconditioner

193.

Haemophilus aphrophilus

No H and V

217.

Leptospira

Weil disease

194.

Haemophilus influenzae

Pfeiffer's bacillus

195.

Haemophylus ducreyi

Soft chancre

196.

HCV RNA

Hepatitis C test of choice

197.

Helicobacter pylori

Peptic ulcer/cancer

189.

Group C,F,G

Mx: cocci in chains Catalase: (-) Colony: pinpoint SXT: (S)

190.

Group D

Beta/alpha/gamma hemolytic streptococcus