RTT Review PDF

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RTT Review...

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Replication, Transcription, Translation Review Replication 1. What is the site on the DNA where replication begins? 2. From above, which direction does replication proceed? 3. Identify the leading and lagging strands in the direction(s) of replication. 4. What protein recognizes the answer to 1? 5. What proteins prevent DNA strands in the replication bubble from reannealng? 6. What specific bacterial enzyme relieves supercoiling? (Topoisomerase is not specific enough) 7. What is the enzyme catalyzing strand elongation? 8. What are the DNA fragments formed on the lagging strand called? 9. What enzyme joins these fragments? 10. How does the cell prevent errors in replication (two ways)? 11. What is the direction of DNA synthesis? 12. Is DNA replication conservative, semi-conservative, or dispersive? 13. Can DNA polymerase start polymerization de novo? Transcription 1. What is transcription? 2. What enzyme catalyzes transcription? 3. Where does RNA polymerase bind to the DNA to start transcription? 4. What give RNA polymerase specificity for binding the promoter in bacteria? 5. Are all RNA transcripts used to make protein? 6. What does polycistronic mean? 7. What is an operon? 8. Is bacterial mRNA stable (may need to refer to your book on this one)? 9. What is a promoter? Translation 1. What catalyzes translation? 2. T/F In bacteria, translation can begin before transcription of the mRNA is complete. 3. What sequence does the ribosome recognize and bind to in bacteria? 4. What part of the ribosome binds the Shine Dalgarno sequence? 5. What type of bond is formed between the amino acids to make a protein? 6. What signals the start of translation and the end? 7. What is the first amino acid to start translation of every bacterial peptide? 8. How many nucleotides are in a codon?...