The correct laboratory technique in using centrifuge PDF

Title The correct laboratory technique in using centrifuge
Course Chemistry
Institution Centro Escolar University
Pages 1
File Size 35 KB
File Type PDF
Total Downloads 544
Total Views 705

Summary

Download The correct laboratory technique in using centrifuge PDF


Description

ANACHEMLAB M1ACT3 1. Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. The correct laboratory technique in using the centrifuge for qualitative analysis is that you use the right tube in the centrifuge and you need to balance the solution of the tubes when you insert it in the centrifuge, and it must be placed in the opposite durection. Also you need to close the rotor lid. And you need to set the right settings for the centrifuge, so that your tubes will not break. 2. Explain how calcium ions are confirmed present in a solid salt by using flame test. The confirmation of calcium ions are the white precipitate of calcium carbonate formed in the group analysis dissolves in hot dil. acetic acid due to the formation of soluble calcium acetate. 3. Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. a) A method to confirm the pressence of phenol is dissolve the given organic compounds in water. Then add a neutral solution of ferric chloride slowly dropwise. After that observe the change in colour, a red, blue, green or purple coloration indicates the presence of phenol. b) To confirm the presence of ketone in a compound dissolve sodium nitroprusside in distilled water in a clean test tube. Add 1ml of the given organic compound to be tested. Shake well and add sodium hydroxide solution dropwise. If there is the appearance of red colour then the presence of ketone is confirmed. 4. How are proteins confirmed present in a sample? Explain the laboratory process. To confirm the presence of proteins in a sample add the food samples of your choice into the test tubes. Add 1-2ml of ninhydrin solution to it and shake the test tube. Boil the mixture and observe the change. If there is the appearance of deep blue or purple color then the presence of protein is confirmed. References: http://amrita.olabs.edu.in/?sub=73&brch=7&sim=180&cnt=1 https://byjus.com/biology/test-for-protein/ https://byjus.com/chemistry/test-for-phenolic-group/ https://byjus.com/chemistry/tests-for-aldehydes-and-ketones/...


Similar Free PDFs