Unknown Bacterial Identification - Sample #1 PDF

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Summary

Unknown Bacterial Identification...

Description

6/20/2021

Laboratory Simulation

CASE REPORT: While swimming in the creek, Anna slipped and a rock cut a three-inch gash in her leg. The next day, the area around the cut was swollen and red and the pain was increasing. When she began to run a fever later in the afternoon and the wound began to throb, a friend took her to the emergency room. A specimen of pus was collected from the wound for culture. An IV was started, delivering a broad-spectrum antibiotic. Blood work showed high activity and numbers of white blood cells. Quick work by the laboratory team isolated the organism into pure culture and was provided to you on a nutrient agar slant and in a liquid broth culture tube for further investigation. Patient condition is becoming critical. Unknown bacterium identification needed STAT.

Student: Aliena

Case Introduction Questions Based on the information in the case, what organism(s) could or should be suspected to cause this scenario? Select all that apply. Pseudomonas aeruginosa Staphylococcus aureus Escherichia coli

Now that a pure culture has been isolated for you, what would be a good starting procedure to begin classifying your unknown organism? Gram staining

Collected Lab Data Test

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Result

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6/20/2021

Laboratory Simulation

Test

Result

Gram staining

Gram (-) bacilli Oxidase test

Glucose fermentation

Nitrate reduction Unknown bacterium identification

Blue color was noted on swab, unknown bacteria was concluded to be oxidase (+) Red appearance indicates no colorimetric pH change was noted in PRB tube, unknown bacteria was concluded to be glucose fermentation (-) Colorless reaction after zinc addition was noted in tube, unknown bacteria was concluded to be nitrate reduction (+)

Pseudomonas aeruginosa

Completed Dichotomous Key

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Laboratory Simulation

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