Week 10 Post-class Assessment-Sec U09 PDF

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Week 10 Post-class Assessment-Sec U09 biology lab...

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General Microbiology (MCB 3020L): Fall 2020 Week 10 Post-class Assessment (20 points) Section U09 Week Topic _________________________________ Date __11/04/20____ Section # ________ Student Name ______ ______ Panther ID # _______ In a Word document, use the prompts below to conduct research and answer each of the questions based on this week’s experiments. Remember to use full sentences and proper citations for each of your answers. References section required at the end of assignment. 1. Fill out the following table based on instructions given during your class section. Remember to download and zoom plate pictures to get an accurate measurement. (7 points) Bacteria sp.

Acinetobacter baumannii Acinetobacter lwoffi Aeromonas hydrophilia Aeromonas sobria Enterobacter cloacae Escherichia coli Klebsiella oxytoca Klebsiella pneumoniae Proteus penneri

Antibiotic #1

Antibiotic #2

Antibiotic #3

Antibiotic #4

CL 100 CL 100 TIM 85 TIM 85 CL 100 CL 100 CL 100 CTT 30 CL 100 VA 5 CL 100

NOR 10 NOR 10 CN 10 CN 10 AMC 60 AMC 60 AMC 60 AMC 60 AMC 60 IPM 10

AMP 25 AMP 25 IPM 10 IPM 10 CTT 30

AMC 60 AMC 60 AMP 25 AMP 25 IPM 10 SF30 0 CTX 5 IPM 10 CTX 5

Staphylococcus aureus Staphylococcus saprophyticus Stenotrophomona P0.5 s maltophilia Key: R - Resistant I- Intermediate S- Susceptible

R S S

S

W5 W5

I S

R

W5 IPM 10 IPM 10 IPM 10 FOX 10 NOR 10 IPM 10

R S

I

E5 NV 5 CIP2 .5

S S R

Antibiotic #5

Antibiotic #6

F200

R

W5

S

S

W5

I

SF 300 CIP 2.5 CIP 2.5 AMP 25 AMP 25 AMP 25 ATM 30 AMP 25 TE 30 CTX 5 ATM 30

CTX 5 CTX 5 CTX 5 CN 10 CAZ 10 CTX 5 CTT 30 CIP 2.5

S

F200 TIM 85

2. Based on your table above, when examining the CL100 antibiotic, which species of bacteria would be effectively treated with this antibiotic? Which species would not? Why? (2 points) 3. Would Gram positive or Gram negative bacteria be more susceptible to antibiotics? Why? (2 points)

Notes

4. You have identified your ‘bacterial unknown’ from this semester and now its antibiotic assay. Which antibiotics is your bacteria susceptible towards? Do these patterns of antibiotic resistance/susceptibility make sense based on the identification of your bacteria? Why or why not? (4 points) 5. Using an example, explain how the Kirby-Bauer methods can be useful in a clinical setting. Why would you not want to prescribe an antibiotic that is classified as “intermediate”? (2 points) Grammar: 1 point In-text citations and references: 1 point Class activity confirmation photos: 1 point Reminder: Attach your screenshot of this week’s activities at the end of your Post-class Assessment for credit....