Assn 5 - BQMOC of paper for project 6 with Dr Amanda Schivell PDF

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BQMOC of paper for project 6 with Dr Amanda Schivell ...

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1183457/pdf/bj3880785.pdf

This paper is relevant for our model because it introduces a new protein (80K-H) into the system that links it to a kinase (PKCζ ) and a known player of GLUT4 vesicle trafficking (munc18c)- bringing our model all together. Insulin stimulation in either adipocytes and muscle cells, triggers an interaction between PKCζ , 80 KHa n dmu nc 1 8ct of o r mac o mpl e x t ha t i nt u r nf r e e ss p e c i ficme mbr a nep r o t e i nst oe n ha n c eGLUT4t r a n s l oc a t i ont ot he me mbr a ne .PKCζi ss i mi l a rt oo urp r e vi ou s l ys t u di e dki na s e sa si tph os ph o r y l a t e s80 KHa t s e r i nea n dt h r e on i nes i t e sa n di sc o nt r o l l e db yPI 3K. Th epa p e ra l s ou s e se x pe r i me nt a l t e c h ni q ue swea r ef a mi l i a rwi t h -i mmu no pr e c i p i t a t i on , DNAc o ns t r uc t s , i mmun ob l ot s -t o i l l u s t r a t et he i rs i g ni fic a ntr ol ei nt heg l uc o s e / i ns u l i ns i gn a l l i n gpa t h wa y ....