Beer Lambert tutorial questions PDF

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Practical Skills for Biosciences 2 Beer Lambert Law Tutorial

1. A solution of eosin of concentration 0.0004M was measured in a spectrophotometer cuvette with a 1cm light path at 570nm. What would be the expected absorbance given eosin has a molar absorbance of 3090 l mol-1 cm-1? 2. A 0.0002M solution of ATP was added to a spectrophotometer cuvette with a 1cm light path and was read at 259nm. Given ATP has a molar absorbance of 15400 l mol -1 cm-1, what would be the expected absorbance? 3. A 0.35mM solution of NADH was added to a spectrophotometer cuvette with a 1cm light path and was read at 340nm. If the molar absorbance of NADH is 6220 M -1 cm-1, what absorbance value would be expected? 4. A 0.49mM solution of safranin was added to a spectrophotometer cuvette with a 1cm light path and was read at 570nm. If safranin has a molar absorbance of 2.06 x10 3 l mol-1 cm-1, what would be the expected absorbance? 5. A solution of NADH has an absorbance (A) of 0.316 at 340 nm when measured in a cuvette with a 1cm pathlength. The molar absorbance of NADH is 6220 l mol-1 cm-1. What is the concentration of the solution? 6. A solution of ATP at 259nm in a 1cm cuvette has an absorbance (A) of 0.062. The molar absorbance of ATP is 15400 l mol-1 cm-1. What is the concentration of the solution in micromoles? 7. The molar absorbance of ATP is 15400 l mol-1 cm-1. What is the concentration of a solution of ATP when measured at 259nm in a cuvette with a 1cm pathlength and an absorbance of 0.587? Give your final answer in M rounded to 1 decimal place. 8. A 6.25 x 10-5 M solution of NAD+ has an absorbance of 0.900 at wavelength of 260nm. What is the molar absorbance of NAD+? 9. A colourless solution of concentration 0.003mol/l has an absorbance of 0.658 at its peak wavelength. What is its absorption coefficient? 10. A red coloured solution has an absorbance of 0.856 at its peak wavelength. If the concentration is 4.5mmol/l, what is the absorptivity coefficient of the solution?...