BIO 270 LAB PART I DISSECTION OF RAT PDF

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answer for dissection of rat workbook. This workbook is the Part 1 of Lab Report BIO 270 for semester 3 student who take AS120....

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BIO270 / OKT 2021 -FEB 2022

UNIVERSITI TEKNOLOGI MARA (UiTM) Branch Pahang Campus Jengka

BIO 270 ANIMAL & PLANT PHYSIOLOGY Laboratory Part 1 Dissection of Rat Student Name Student ID Group/Class H/P Number Email Address Lecturer Name

SITI AISHA QHAIRUNNISSA BINTI ROSLAN 2020855836 AS1203B 0199188269 [email protected] DR. ENGKU AZLIN RAHAYU BINTI ENGKU ARIFF

FOR LECTURER USE ONLY

Section Part I

Mark /40

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BIO270 / OKT 2021 -FEB 2022

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Instructions: 1. This workbook must be completed and submitted individually. 2. Complete this workbook. The total marks of this workbook are 40 marks. 3. You are given links to several videos and website. Watch the videos and website before answering the workbook questions. 4. You may use other reliable information to assist you when necessary. 5. Should you need any further clarification about the assignment, please consult with your lecturer. 6. Answer ALL questions in this workbook. DO NOT alter this workbook, please use the space given to write your answer accordingly. 7. After completing the workbook, each student must save his/her workbook in pdf before submitting it to their respective lecturers via an online platform before the due date. 8. Failure to submit this workbook may affect your continuous assessment marks.

TOPIC

LINK

How animals are used in research

https://www.youtube.com/watch?v=U9OiEAWbU-M

1.

2.

Humane killing

https://www.understandinganimalresearch.org.uk/animals /three-rs/ https://youtu.be/2Du0h270a-c

3.

Dissection procedure

https://youtu.be/9Xk2KCYTujk

4.

Interview with the Veterinarian

https://youtu.be/ezt9p00PSOg

Carcass Disposal

https://youtu.be/VDiGd4620ac

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HOW ANIMALS ARE USED IN RESEARCH 1. From the video https://www.youtube.com/watch?v=U9OiEAWbU-M, name the animal and state their importance in research. (6 marks) Animal (0.5 mark each)

Importance in Research (1 mark each) -

Mice are used to research into cancer, human cancer can be given to mice.

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Zebrafish are used to study tuberculosis and stroke.

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Dogs lungs are similar to human lungs so dogs are used to test asthma drugs.

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Monkeys are used to study vision because they have binocular vision like humans.

Mice

Zebrafish

Dog

Monkey

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HUMANE KILLING 1.

After some procedures, it may be necessary to kill the animal to prevent further suffering. The person who conducted the procedure must ensure that the animal is killed competently and humanely. List out the appropriate methods used in humane killing for the animals in research (2 marks). Refer to website https://www.understandinganimalresearch.org.uk/animals/three-rs/ 1) Replace the use of animals with alternative techniques, or avoid the use of animals altogether. 2) Reduce the number of animals used to a minimum, to obtain information from fewer animals or more information from the same number of animals. 3) Refine the way experiments are carried out, to make sure animals suffer as little as possible. This includes better housing and improvements to procedure which minimise pain and suffering and for improve animal welfare.

2. From the video https://youtu.be/2Du0h270a-c, what method is used to sacrifice the animal? (1 mark)

The method that was used to sacrifice the animal was exposing the animal to Carbon Dioxide gas.

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DISSECTION PROCEDURE 1. After viewing the video https://youtu.be/9Xk2KCYTujk, the dissected rat is illustrated in Figure 1. Label the parts in the box provided below (10 marks)

Figure 1

A B C D E

Organ (1 mark each) Trachea Lung Diaphragm Liver Small intestine

F G H I J

Organ (1 mark each) Large intestine Spleen Stomach Heart Esophagus

2. From the video, what are the organs that were identified/observed BUT were NOT shown/stated in the Figure 1 (4 marks). 1.Kidney 2. Urinary bladder 3. Uterus 4. Ovary

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Fill in the columns with the appropriate answers (8 marks). Organ System Example: Circulatory

Respiratory

Digestive

Main Components (1 mark each) Heart, blood vessels, blood

nose, mouth, throat, Deliver oxygen into the body and voice box, windpipe, and distribute or remove waste gases including carbon dioxide. lungs. mouth, esophagus, stomach, small intestine, large intestine, rectum and anus. Ovaries, testes

Reproductive

Excretory

Main Function (1 mark each ) Internal distribution of materials such as O2, CO2, ions.

Motility, digestion, absorption and secretion are the four vital functions of the digestive system. Gamete production, promotion of fertilization and support developing embryo

Kidneys, ureters, urinary Disposal of metabolic bladder, urethra regulation of osmotic And balance of blood

wastes,

3. Based on your understanding, identify the part of the body that can be used to collect blood without killing the animal (3 marks). 1.Hand 2. Neck 3. Thigh

4. From the veterinarian surgeon point of view, https://youtu.be/ezt9p00PSOg. list out a few challenges faced by a veterinarian (3 marks). 1) Handling wildlife animals or zoo because they are not being domesticated and not clean. They can also be fierce and aggressive. 2) Facing animals that have many diseases such as cancer or tumour. 3) Animals that are fierce and aggressive.

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CARCASS DISPOSAL 1. The carcass must be disposed of properly. By referring to the video, describe how you would dispose of the carcass in the lab (3 marks). https://youtu.be/VDiGd4620ac 1) Firstly, the carcass needs to be put into a bio-hazard bag. 2) Then the bio-hazard bag needed to be sealed or tie tightly. 3) Then, the carcass needed to be stored in animal carcasses freezer. Make sure that the temperature is set to -40 ℃ . 4) Recorded the details of user, date, time, number of carcasses of animal and the type of the animals into the freezer book log. 5) Lastly, the carcasses will be taken by the clinical waste contractor every two weeks to be collected and disposed of.

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ACKNOWLEDGEMENT We would like to thank all the teaching team and lecturers in charge of BIO270 Animals and Plants Physiology UiTM for their expertise and suggestions in ensuring these assessment activities are a success. Here, we also acknowledge, with special thanks, to Dr Nursakinah Latifi and Mr Muhammad Fadhli Mohd Azahar from Laboratory Animal Facility and Management (LAFAM) as well as Madam Nina Keterina Hashim from Faculty of Health Sciences, UiTM Puncak Alam for their involvement and production of the video demo. REFERENCES https://www.youtube.com/watch?v=U9OiEAWbU-M https://www.understandinganimalresearch.org.uk/animals/three-rs/

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Thank You

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