BMS533 Practical 1 March 2021 PDF

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GROUP : AS201 TEAM :Practical 1 : Hazard characterisationLEADER : Nur Asyikin Binti Abd Azizi (2020449544) Members : Dayang Allysa Shazmeen Binti Abang Abd Hadi (2020461856) : Nor Atikah Binti Hamdi (2020861766) : Syaidatul Annisha Binti Sarum (2020837268)Date : 15th April 2021Report writing :This s...

Description

BMS533 Biorisk Management

GROUP : AS201

G2

TEAM :

*Give your group a name

Practical 1 : Hazard characterisation LEADER : Nur Asyikin Binti Abd Azizi (2020449544) Members : Dayang Allysa Shazmeen Binti Abang Abd Hadi (2020461856) : Nor Atikah Binti Hamdi (2020861766) : Syaidatul Annisha Binti Sarum (2020837268)

Date

: 15th April 2021

Report writing : This section

was written by

and reviewed by

Introduction

:

Dayang Allysa

Nor Atkah

Methods

:

Nur Asyikin

Dayang Allysa

Results

:

Nor Atikah

Syaidatul Annisha

Discussion

:

Syaidatul Annisha

Nur Asyikin

BMS533 Practical 1 :

Hazard characterisation

Introduction Hazard characterization is a term that describes the relationship between the amount of pathogen a person has ingested and exposed (dose) and the likelihood of them to develop negative health outcome such as getting severe infection as a response. The severity and the duration of a person to have the infection depends on the dose ingested. The higher the dose, the higher the probability and severity of the illness. Characterizing a risk as clearly as possible is important as it can prevent future injuries and illnesses from happening as we are able to find ways to reduce unwanted incidents from occurring. Occurrence of incidents will be reduced as clear risk characterization on potential hazards will establish new guidelines and safety practices in the workplace which serves as a training tool that can increase proactivity among the workers.

Objective 1.

To identify possible biosafety hazards in a laboratory.

2.

To characterise hazard identify in the lab and define the associated risks

Materials 1. Escherichia coli

Methods A layout of the map was sketched as below :

One biological agent has been picked which was Escherichia coli. The agent picked was used to conduct 3 experiments which were: a. diagnostic procedures (gram staining) b. culture in 1 liter volumes for growth studies c. inoculating mice with the bacteria for pathogenic studies In conducting those experiments we identified 3 biohazards that occurred in the laboratory. Thus, we completed the Hazard Characterisation Sheet that has been attached in this lab report and in addition of that we searched any other information on the internet regarding the hazard that has been identified. Apart from that, we also identified the risks that can occur due to the hazard that has been listed. The risks that was found by our group was discussed together either it can result other consequences. We also identified who will be at risk if the hazard occur in the laboratory or outside laboratory.

Results Table 2.1: List of risks and hazard characterisation Hazard

Risk What can happen

Under what conditions can this happen

Who is at risk

1 Escherichia coli

Infection

In contact with sample

Lab workers

2 Escherichia coli

exposure to pathogen

Not using any protection such as gloves,lab coat and mask

Lab workers/ support workers/ community

3 Escherichia coli

Unintentional release of phatogen

The sample is spilled through laboratory apparatus for example like spatula or inoculating scoop

Lab worker/ Community

4 Escherichia coli

Generation of aerosol

By opening the culture in laminar flow cabinet carelessly

Lab worker/ Support workers

5 Escherichia coli

Contaminated surfaces

Gloves are not worn properly

Lab Workers

Discussion Based on table 2.1, the risks could happen when did not handling this type of bacteria very well during the laboratory. First, this bacteria can cause infection when the lab workers come in contact with the sample. Next, when handling any microbes, those who did not wearing any protection such as gloves, lab coat and mask increase the chances of exposure to the pathogens hence unintentionally spread towards another lab workers, the hence worker and the community. Alongside, when the sample is spilled through the laboratory apparatus for example the spatula or the inoculating loop, this will cause involuntarily release the pathogen and affect the lab workers carry on by the community. When handling microbes, it is important for us to do the culture in the laminar flow cabinet. This is because, the laminar flow cabinet can prevent any contamination towards the lab worker. Last but not least, gloves and mask are needed to worn properly so the contamination surface could be less. In the lab, the uses of bacteria, viruses, blood, tissue, or bodily fluids may pose a biological risk. All of these components have the potential to bear disease or dangerous allergens, putting the lab group at risk. The effects of diseases and allergens may be immediate or take a lot of time to manifest, highlighting the importance of providing adequate protection to all members of the lab team. Preventing a biological emergency in the lab begins with proper storage and security. Exposure to the risk can be reduced by wearing adequate protective equipment and holding biological agents confined in designated areas. There should be systems and processes in place for the safe use, handling, storage, and transportation of

biological hazards. Proper sanitation, such as disinfecting work surfaces and properly disposing of waste, is also important for reducing biological risk. Emergency procedures should be planned beforehand, with the ultimate purpose of eliminating the biological threat and reducing the danger to people and the environment. Proper mechanisms, such as informing others, isolating the region, evacuation, requesting assistance, preventing the spread of contaminants or spills, and decontaminating the work area, may be needed depending on the situation. By doing this experiment, we able to know what are the biohazard and apply the biosafety hazard in the laboratory. It is important for us to always work in safe and good practices during the experiment. One wrong step could be a disaster risk. We should always follow the laboratory security procedure such as while handling with the microorgnism.

Conclusion

In conslusion, it is important to know, learn, and practice the biosafety hazards for our own good and especially towards the people and the environment. The objective of this laboratory are achieved. The possible biosafety hazards in a laboratory is identified.

References MarcusCannon. (2016, September 14). Laboratory Hazards: Chemical, Physical & Biological Hazards in the Lab. InterFocus Lab Furniture; InterFocus Lab Furniture. https://www.mynewlab.com/blog/working-in-a-laboratory-the-hazards-andrisks/#:~:text=The%20use%20of%20bacteria%2C%20viruses,the%20lab%20team %20at%20risk. Dennis, S. B., Miliotis, M. D., Buchanan, R. L., & Food, U. S. (2002). Hazard characterization / dose – response assessment. Microbial Risk Assessment in Food Processing, 77–99. https://doi.org/10.1533/9781855736689.1.77 Weekes, J. (2017). 5 Benefits of Conducting Risk Assessments. Health & Safety Handbook. Retrieved 25 April 2021, from https://healthandsafetyhandbook.com.au/bulletin/5-benefits-of-conducting-riskassessments/.

Lab Report Rubric PRAC # 1

Attribute Marks 1. Punctuality

(include this with every report)

Exceeds Expectations

Meet Expectations

Below Expectations

5

3 --> 4

0 --> 2

Submitted early

Submitted on time

5

Good, clear format

Loose format

Not organized 5

Is the prescribed format used?

4. Introduction Did the students provide background materials? 5. Outcomes

Tot al

Submitted late

Was the report submitted on time?

2. Organization of report

Marks

Good, concise summary of background

OK. Materials included but not summarised or too long.

Inadequate summary /duplicated from sources.

Well defined.

Acceptable, student is a bit unsure

Not well define. Student is unsure.

5

Risk1 5

Specific? Well defined?

5. Outcomes

Well defined.

Acceptable, student is a bit unsure

Not well define. Student is unsure.

Risk2 5

Specific? Well defined?

5. Outcomes

Well defined.

Acceptable, student is a bit unsure

Not well define. Student is unsure.

Risk3 5

Specific? Well defined?

5. Outcomes

Well defined.

Acceptable, student is a bit unsure

Not well define. Student is unsure.

Risk4 5

Specific? Well defined?

5

6. Results Figure a. Is Figure in proper format?

Well formatted, clear

Proper format

Not formatted

7. Discussion

Results were well discussed.

Some attempts. mainly descriptive.

No attempt to discuss.

Correct conclusion, related to the objective

Conclusion is provided, but not directly related to the objective

No conclusion/ conclusion not related to objective(s)

5

Is a discussion inlcuded?

8. Conclusion Was a conclusion provided?

SUM

Evaluated by

:_________________________________________ Date : ________________________

5

50...