BSC2426 Syllabus Fall 2019 1985 PDF

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BSC2426 – Biotechnology Methods and Applications I (3 credits) Fall 2019 (2197) Term (August-December 2019) Reference #1985 Day/Time:

Lecture: M 2:25 - 3:40 PM, Online: R 2:05 - 3:20 PM

Classroom:

Building A Room A102

Instructor:

Professor: Tammy Laberge Location: A349 On Campus: (305) 237-8118 E-mail: [email protected]

Office Hours:

Mondays: 9:00 – 10:00 AM, 1:30 – 2:25 PM, 3:50 – 4:55 PM Tuesdays: 2:05 – 4:40 PM Wednesdays: 9:00 – 1:25 AM

Course Description: The Biotechnology Methods and Applications I course is a Blended course that addresses the basic principles, concepts and techniques of biotechnology necessary for an understanding of the field, and effective work in a pharmaceutical-, biotechnology- and/or research-laboratory setting(s). Practical applications of biotechnology are explored. Goals and Objectives: Upon successful completion of this course, students will be able to demonstrate knowledge of: 1. The field of biotechnology with respect to the branches of science involved, the history of biotechnology, possible career choices and the future of the biotech industry 2. Biotechnology workplace and safety regulations 3. Cell Structure 4. The use of microorganisms in biotechnology 5. The basic principles of recombinant DNA technology 6. The importance of protein and the methods for their production, isolation and purification 7. Understanding of Genomics 8. Molecular data collection and manipulation 9. Legislation employed in the regulation of biotechnology Page 1 of 8

Resources Textbook:

Daugherty, Ellyn. Biotechnology: Science for the New Millennium, 2nd edition.

Additional Resources:

Dicky, Jean; Hogan, Kelly; Reece, Jane; Simon, Eric J. Biology (with Physiology chapters) 6th Edition. Reece, Urry, Cain, Wasserman, and Minorsky. Campbell Biology, 11th edition (Older versions can also be used). Thieman, Palladino. Introduction to Biotechnology 3rd edition. Mader, Windelspecht. Essentials of Biology, 3rd edition.

Blackboard:

Your Blackboard link for this course provides links to the course syllabus, PowerPoint lecture outlines, practice questions for the on-line quizzes and tests for the chapters to be covered during the course.

Learning Support:

Computer support and tutoring are available in the Learning Resources Lab and Computer Courtyard located in 2102 and 2201.

Class Procedures Attendance:

There will be a sign-in sheet for every class to verify attendance. If you cannot attend class, please notify the instructor via email in advance. The instructor reserves the right to withdraw students with more than three (3) unexcused absences. All the tests and assignments MUST be done. Non-attendance to tests MUST be justified, preferably prior to the absence

Personal Communication Devices: Pagers and cellular phones are NOT conducive to the educational process in this class. I will assume that any interruption due to a personal communication device will be justifiable based on a real emergency and that the student being summoned will need to leave immediately to deliver a baby, attend to the dying, retrieve an injured child from daycare, or otherwise take immediate action which necessitates leaving. Such devices used during any quiz or exam will result in an automatic zero.

Review Sessions:

The lecture notes in PowerPoint and related topic links are available on Blackboard. Exam questions will be drawn from questions in the online homework, lectures and related links.

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Components:

60% Exams: three exams covering 3-5 topics each in multiple-choice and true-false format. Students will be given one hour to complete the exam. Exams grades will be returned the following class and will not be graded on a curve. Except for unusual circumstances, no make-up exams will be provided. Therefore, if you miss an exam, a zero will be recorded for that particular exam. If you anticipate missing an exam, you must arrange with the professor to take the exam early. Students must bring to class their own scantron sheet; the Professor will not provide these sheets. The specific scantron that will be utilized in the course will be discussed by the Professor, if you missed this information, please contact the Professor through email. 20% Online Quizzes: There are online quizzess for each topic available on Blackboard that must be completed by 5:00 PM the day before an exam. Questions will consist of multiple-choice, matching, fill in the blank and true-false questions for each chapter that are similar to some the actual exam questions. 15% In-Class Quizzes: There are in-class quizzes that will be given that will test your knowledge of each topic. Quizzes will consist of 10 multiple choice questions that will be given at the end of class. The best 10 out of 12 scores will count towards your quiz grade. There are NO make-up quizzes for any missed quizzes. 5% Participation: a sign-in sheet will be available to verify attendance. Each class counts for part of the participation points towards your final grade. You are expected to have read the material that will be covered before lecture and to participate in class discussions.

Grading Scale:

A = 90-100, B = 80-89, C = 70-79, D = 60-69, F = 0-59 No pluses or minuses will be given. It is the responsibility of the student to withdraw from the course by the appropriate deadline if the student wishes to receive a grade of W.

Service Learning:

Service learning opportunities will be offered for students that want to get bonus points in this course. The service chosen must be biologically relevant in order to receive extra credit. The Center for Community Involvement web site (www.mdc.edu/cci) provides further details on service learning.

Scantron Form OMB-882 (required)

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Important Dates for the Fall 2019 (2187) Friday, August 30:

Last Day to Change Courses without Penalty; Withdraw from classes with 100% refund; Register, add, drop, or change sections of credit courses with instructor or department approval

Monday, November 4:

Last Day to Withdraw with a Grade of W

Friday, December 13:

Last Day of Classes for the Fall 2019-1 Term

BSC 22425 – Biotechnology Methods and Applications I Fall 2019 Tentative Course Schedule Week

Day

1

26-Aug

2 3 4 5 6

2-Sep 9-Sep 16-Sep 23-Sep 30-Sep

7

7-Oct

8 9 10 11 12 13 14 15 16 17

14-Oct 21-Oct 28-Oct 4-Nov 11-Nov 18-Nov 25-Nov 2-Dec 9-Dec 16-Dec

Topic Introduction/ What is Biotechnology/ The Nature of Science No Class Chemistry of Life Macromolecules Cell Structure & Function Exam 1 Nucleic Acid Structure & Analysis Replication & Recombination Transcription Translation: Protein Synthesis Gene Mutation & DNA Repair No Class Exam 2 Microorganism & Biotechnology Nucleic Acids Techniques Genomics & Biotechnology Exam 3

Access Students: Any Student with a disability who would like to discuss special ways that I can be of assistance should contact me by phone or in person after class. Your privacy will be respected and I will work with you to make your class experience a successful one. If you will require any Special Accommodations due to your disability, please see ACCESS SERVICES, Room 6112, (305) 237-1272 or Page 4 of 8

http://www.mdc.edu/north/accessservices/ Exam #1 Material Modules 1, 2 and 3 Introduction/ What is Biotechnology/ The Nature of Science Chemistry of Life Macromolecules Cell Structure and Function

Exam #2 Material Module 4 Nucleic Acid Structure and Analysis Replication and Recombination Transcription Translation Gene Mutation and DNA Repair

Exam #3 Material Module 5 Microorganisms and Biotechnology Nucleic Acid Techniques Genomics and Bioinformatics

BSC 2426 – Biotechnology Methods and Applications Lecture (3 credits) Course Competencies Competency 1: The student will demonstrate knowledge of the field of biotechnology with respect to the branches of science involved, the history of biotechnology, possible career choices and the future of the biotech industry by: 1. Defining the concept of biotechnology. 2. Explaining different biotechnology specialties and the scientific disciplines contributing to each. 3. Listing examples of historic and contemporary products from biotechnology applications. 4. Describing the history of domestication and agriculture as well as the history of fermented foods and beverages. 5. Listing milestones of modern biotechnology development. 6. Describing basic methods and applications of modern biotechnology. 7. Demonstrating basic methods and applications of modern biotechnology. 8. Summarizing the organization and functions of biotechnology companies. 9. Describing biotechnology workplaces. 10. Defining important skills and training required to become part of the biotechnology workforce. 11. Discussing career opportunities and hiring trends in biotechnology. Competency 2:The student will demonstrate knowledge of biotechnology workplace and safety regulations by: 1. Defining safety rules in the workplace. 2. Describing hazards and risk assessment. 3. Naming regulatory agencies. 4. Describing Occupational Safety and Health Administration (OSHA) worker safety regulations. 5. Defining principles of labeling, documentation, and housekeeping practices. Page 5 of 8

6. Summarizing actions directed to reduce risk in the laboratory setting. Competency 3: The student will demonstrate knowledge of cell structure by: 1. Describing the structures and functions of prokaryotic cells. 2. Describing the structures and functions of eukaryotic cells. 3. Listing examples of prokaryotic and eukaryotic cells. 4. Comparing and contrasting prokaryotic and eukaryotic cells. Competency 4: The student will demonstrate an understanding of the use of microrganisms in biotechnology by: 1. Describing the features of bacteria that make them useful tools for many applications in biotechnology. 2. Listing examples of how yeast can serve valuable roles in biotechnology. 3. Differentiating between alcoholic and lactic acid fermentation and discussing the importance of each in the production of common foods and beverages. 4. Demonstrating principles of microbial fermentation. 5. Describing how microorganisms play an important role in the development and production of recombinant proteins. 6. Listing examples of therapeutic proteins. 7. Identifying microorganisms that may pose a threat as bioweapons. 8. Categorizing biotechnology strategies that may be used to detect, identify, and combat bioweapons. 9. Describing the features of viruses that make them useful tools for many applications in biotechnology. Competency 5: The student will demonstrate knowledge of the basic principles of recombinant DNA technology by: 1. Describing the basic structure of DNA, RNA, and proteins. 2. Demonstrating the principles of DNA isolation. 3. Describing methods to specifically cut and join DNA. 4. Describing methods to separate restriction DNA fragments and visualize DNA. 5. Comparing methods of DNA cloning in different types of cloning vectors. 6. Describing different methods of cell transformation, transient expression and transfection. 7. Explaining the use of DNA libraries. 8. Comparing Southern, Northern and Western blot hybridization. 9. Demonstrating the principles of the Polymerase Chain Reaction. 10. Listing the various methods of DNA sequencing. 11. Explaining the principles of Sanger’s method of DNA sequencing 12. Defining methods of DNA microarray technology. 13. Describing recombinant DNA production systems used to generate proteins. 14. Listing examples of therapeutic proteins generated using recombinant DNA technology. 15. Describing the use of recombinant DNA in the food industry. 16. Summarizing the ethical issues associated with the utilization of recombinant DNA technology in food development and safety. Competency 6: The student will demonstrate an understanding of the importance of proteins and the methods for their production, isolation and purification by: 1. Describing the qualitative analysis of proteins. 2. Describing the principles of protein extraction and purification. 3. Describing chemical assays such as turbidity, viscosity and density of proteins. 4. Describing the features unique to E. coli that lead to its frequent use in protein production. 5. Describing methods employed in sequencing short peptides and proteins. 6. Explaining proteomics and how it may be applied to protein research and development.

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Competency 7: The student will demonstrate an understanding of genomics by: 1. Explaining differences between genetic, cytological and physical maps. 2. Demonstrating knowledge of applications for the human genome project. 3. Listing other genome projects. 4. Discussing the ethical, legal and social implications of the Human Genome Project. Competency 8: The student will demonstrate knowledge of molecular data collection and manipulation by: 1. Describing bioinformatics and the utilization of computational information management systems in biological research and dissemination of biological information. 2. Describing databases and websites available for sequence acquisition and analysis. 3. Accessing the location of sequences available in GenBank. 4. Utilizing a webcutter to generate restriction enzyme maps of a DNA sequence. 5. Performing BLAST search for homologous sequences and motifs in DNA sequences. 6. Utilizing the ExPASy proteomic server to translate DNA sequences into amino acid sequences. 7. Designing oligonucleotides of specific length, base composition, and nucleotide sequence by using computer technologies. 8. Calculating the melting temperature for an oligonucleotide. 9. Describing factors that affect oligonucleotide binding and function in PCR and other biotechnology applications. Competency 9: The student will demonstrate knowledge of the legislation employed in the regulation of Biotechnology by: 1. Explaining the roles of federal and state agencies in the development and manufacturing of biotechnology products. 2. Comparing and contrasting the guidelines of federal and state agencies that regulate the biotechnology industry. 3. Explaining the use of state, local, and industry regulations. 4. Explaining the use of current Good Laboratory Practice Standards (GLP) or Good Manufacturing Practices (GMP). 5. Listing the criteria that make a plant eligible for “notification” under governmental guidelines. 6. Explaining the precautions that must be taken to prevent release of bioengineered plants into the environment. 7. Identifying ethical issues pertaining to the development of bioengineered plants. 8. Summarizing the functions of patents. 9. Explaining why DNA sequences are considered patentable.

Academic Integrity: The instructor supports the College’s policies regarding academic integrity and honesty. These include the policies regarding cheating, plagiarism, and fabrication of information. It is your responsibility to understand fully what these policies are. As such, you are encouraged to obtain a copy of the Student Rights and Responsibilities Handbook and read these policies carefully and thoroughly. A. Cheating – Cheating is defined as the improper taking or tendering of any information or material which shall be used to determine academic credit. Taking of information includes, but is not limited to, copying graded homework assignments from another student; working together with another individual(s) on a take-home test or homework when not specifically permitted by the instructor; looking or attempting to look at Page 7 of 8

another student’s paper during an examination and; looking or attempting to look at text or notes during an examination when not permitted. Tendering of information includes, but is not limited to, giving your work to another student to be used or copied; giving someone answers to exam questions either when the exam is being given or after having taken an exam; giving or selling a term paper or other written materials to another student; sharing information on a graded assignment. All class notes, lecture outlines, class assignments, examinations, and any other course information are copyrighted material and may not be copied or distributed in any format or for any purpose without permission from the instructor or the author as the case may be.

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