Coshh Crystal Violet 2017 PDF

Title Coshh Crystal Violet 2017
Course Biomedical Science Practicals 1
Institution University of Wolverhampton
Pages 3
File Size 194.9 KB
File Type PDF
Total Downloads 22
Total Views 166

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COSHH FORM...


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CoSHH: Control of Substances Hazardous to Health By completing this document you are assessing the risk involved in handling, use and disposal of the named substance, and by signing it YOU BECOME RESPONSIBLE for its use. Numbers by each section relate to those in the Safety Data Sheets (SDS). 1) FULL NAME OF CHEMICAL with chemical formula, including ‘common’ name, and grade where possible. (examples: Sodium Hydroxide (NaOH) GPR; Methanol ‘HPLC grade’; Boric Acid ‘ANALAR’) Crystal violet stain 2% Crystal violet in 95% ethanol

PURPOSE FOR WHICH YOU NEED THE SUBSTANCE: (brief description of what you are going to do with it and why) Microbiological stain

QUANTITY /AMOUNT OF SUBSTANCE REQUIRED: (examples: 5g; 250ml; 1000 units; 2 slopes) 20 ml in dropper bottle only 2-3 drops per slide

QUANTITY /AMOUNT OF SUBSTANCE TO BE PREPARED from original above: (examples: 500ml 0.5M solution; 20 petri dishes) Be aware: if what you make is radically different from the original substance A SEPARATE CoSHH FORM WILL NEED TO BE PREPARED. none

I CONFIRM THAT A SAFER ALTERNATIVE CANNOT BE USED NOW REFER TO THE APPROPRIATE SAFETY DATA SHEETS FOR THE SUBSTANCE.

For chemicals use the online site: ‘START’: ‘ALL PROGRAMS’: ‘SAS SOFTWARE’: ‘SAFETY DATA SHEETS’

RPH conversion of MSI 07/10 document

2) IDENTIFIED HAZARDS (EXPOSURE RISK): SEE ALSO BOX “11” (Toxicological Info) in SDS (examples: causes burns; flammable; toxic. Is it harmful to the environment? ie: do not put silver stain in the drains! flammable. Limited evidence of a carcinogenic effect. Risk of serious damage to eyes. Toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment. Wear PPE and gloves.

3)WHAT IS IT? (examples: acid; alkali; solvent; inorganic salt) Alcoholic stain

4) FIRST-AID MEASURES: (What do I do if it gets on me or someone else?) Eye contact: Irrigate thoroughly with water for at least 10 minutes. OBTAIN MEDICAL ATTENTION. - Inhalation: Remove from exposure, rest and keep warm. In severe cases obtain medical attention. - Skin contact: Wash off skin thoroughly with water. Remove contaminated clothing and wash before re-use. Unless contact has been slight, OBTAIN MEDICAL ATTENTION. - Ingestion: Wash out mouth thoroughly with water and give plenty of water to drink. OBTAIN MEDICAL ATTENTION.

5) FIRE-FIGHTING MEASURES: (What do I do, and with what, and where is it kept?) Water spray, dry powder or vaporising liquids

6) SPILLAGE/ACCIDENTAL RELEASE (If I spill it, what do I immediately do? In addition: always tell those around you. INFORM THE TECHNICAL STAFF) Shut off all sources of ignition.(Turn off Bunsens) Warn people to keep away and inform staff

7) HANDLING and STORAGE: (Are there any special instructions to obey? examples: “NEVER ADD WATER TO THIS MATERIAL”; “Avoid contact with skin and eyes”; Do not store with ***”) Avoid contact with skin and eyes.

ALL ORIGINAL CHEMICALS, SOLVENTS AND DILUTED CHEMICALS CONTAINING SOLVENTS MUST BE STORED IN THE APPROPRIATE PLACE IN PREPROOMS ONLY UNLESS TOLD OTHERWISE. DILUTED CHEMICALS MAY BE STORED IN STUDENT CUPBOARDS IN APPRPROPRIATE CONTAINERS.

RPH conversion of MSI 07/10 document

8) PERSONAL PROTECTION: ALL USERS MUST UTILISE A MINIMUM OF FASTENED LABCOAT AND SAFETY SPECS UNLESS ADDITIONAL PROTECTION IS SPECIFIED. Tick as specified by SDS: Disposable gloves Heavy rubber gloves Disposable apron Face Shield Extraction/Flow hood Safety Cabinet Other If other give details: 9) PHYSICAL PROPERTIES: (examples: Is it soluble in water? If not, in what? Is it flammable? Does it have to be stored in special containers, or in the dark? Any other important considerations?) Flammable organic stain

13) DISPOSAL CONSIDERATIONS: (How do I get rid anything I use or make?) DO NOT DISPOSE OF CHEMICALS DOWN THE SINKS UNLESS GIVEN PERMISSION!! If you are in doubt, ASK THE TECHNICIANS. Leave in staining trays for disposal by technicians

ASSESSED RISK: ie, are there any risks involved in using this substance? Flammable keep well away from Bunsen flames. Aquatic toxin do not dispose of via sinks Avoid Skin & eye contact Low with good lab practice including use PPE

Name of Assessor:

Dr Liz O'Gara

Supervisor:

Signature Signature

Date of Assessment: 20/10/17

Date Form Completed

2016-09-09 21:34:53

RPH conversion of MSI 07/10 document...


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