DNA Extraction DOCX

Title	DNA Extraction
Author	Anjanette Paasa
Pages	5
File Size	94.8 KB
File Type	DOCX
Total Downloads	285
Total Views	567

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DNA Extraction in Cheek Cells Marie Anjanette B. Paasa BIO 108.1 - Cell Biology Laboratory (F56), Department of Biological Sciences, College of Science and Mathematics, MSU-Iligan Institute of Technology ABSTRACT DNA as the carrier of genetic information that is inherited and passed down to offspring plays an important role in maintaining the growth and deeelopment of organisms. And also, it determines how organisms look like and also how organism functions and process actieities. Theoretically, DNA is too small and fts within a 2-3 micrometers size of nucleus which appears ineisible with the eye. But this actieity, it followed a procedure that demulsify and disrupt cell that would allow DNA to be seen by the naked eye. Cheek cells, which are easily collected with sports drink, were subjects for DNA extraction. DNA extraction ineoleed (1) the breakdown of lipids or fats components of cell and nuclear membrane to expose the DNA and cell components, (2) the separation of DNA from the wrapping proteins using protease, found in meat tenderizer, to cut proteins which causes the stretching out and unwinding of DNA and (3) the precipitation of DNA with alcohol since DNA is insoluble to alcohol which caused the DNA to clump. Thus, purifcation of DNA away from proteins and other cellular components are necessary to isolate DNA from cheek cells. Aside from extraction of DNA, obsereation on properties of cell, cell membrane and DNA when subjected with different reagents were drawn out in this actieity. INTRODUCTION All cellular organisms on earth haee the genetic code which is called DNA or deoxyribonucleic acid. It contains information which goeerns and controls cellular actieities and biological processes; and regulates deeelopment of organisms. DNA also directs the cells collectieely to function and work properly; and expresses characteristics such as morphological traits, disorders and defciencies (Campbell et. al., 2008). DNA is found within the cell under a compartment called nucleus, which is the control unit and secures the DNA molecules. DNA is basically composed of nucleotides; and each nucleotide consists a nitrogen-containing base, a sugar and a phosphate group. These nucleotides form polymer which is called a polynucleotide that constitute the DNA strand (Darnell et. al., 2003). Two polynucleotide chains winds into a double-helical structure and at the same time maintains a standard base-pairing in which adenosine always pairs with thymine, and guanosine always pairs with cytosine. This property of DNA explains its...