Hand Washing Questions - Lab 2 PDF

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Microbiology Lab Safety PRE-LAB QUESTIONS 1. What constitutes personal protective equipment? When should personal protective equipment be worn? Why is personal protective equipment important?



PPE’s include safety goggles, gloves, lab coat/long sleeve button up, and closed toed shoes. These should be worn when conducting experiments to protect yourself, as well as reduce the risk of contaminating the experiment. This is important because you do not want to put yourself at risk and want your experiment to be as accurate as possible.

2. Why are there increasing levels of biological containment procedures? What level of biological containment will be necessary to complete the experiments in this manual?



There are four different BSL’s for the different risk levels of containment and threat. The BSL-1 is the lowest and this is where we will be to complete the experiments in this manual.

3. What document should you refer to if you have questions regarding the safety or disposal of a chemical? Where can this document be located?



The document is called the Safety Data Sheet. This document has every chemical in our kit listed on it and it is located on the eScience Labs website.

4. List five safety procedures that should always be adhered to when working with microorganisms.



Wash your hands with soap before and after lab.

©eScience Labs, 2018

Microbiology Lab Safety 

Do not eat, drink, chew gum, or smoke while performing the experiments.



Do not put anything in your mouth while performing the experiments.



Prepare a workspace on a nonporous surface, free from food, drinks, or personal objects. Avoiding areas with rugs or carpet.



Disinfect your work area with isopropyl alcohol or 10% bleach solution before and after performing the experiment.

©eScience Labs, 2018

Microbiology Lab Safety EXPERIMENT 1: IMPORTANCE OF HAND HYGIENE Data Tables Table 2: Experiment 1 Colony Growth Plate 1 2

Condition Hand without Washing Hand with Washing

Growth Day 1

Growth Day 2

Growth Day 3

Growth Day 4

Growth Day 5

-

+

++

+++

+++

-

-

-

+

+

3

Yeast

-

+

++

+++

++++

4

Deionized Water

-

-

-

+

+

Post-Lab Questions 1. What purpose did plating the yeast serve in this experiment? What purpose did the water serve?



Plating the yeast was to serve as an effective model for how microorganisms can be transferred into the next plate being just the water. The water did show that there was no growth where the water was on the plate, but there was slight growth on the side of the plate where it could have been contaminated.

2. Which of the two hand treatments grew the most colonies?



Plate #1 was the hand without washing, and it grew the most colonies.

3. What effect does handwashing have on the presence of bacteria?



Handwashing is especially important and decreases the presence of bacteria and spreading it. Plate #2 had a significant decrease in colonies from plate #1. ©eScience Labs, 2018

Microbiology Lab Safety 4. Were there any colonies present on plate #2? What could account for the presence of these colonies?



Yes, there were still colonies present on plate #2. When washing your hands you do not get 100% of the bacteria from your hands, but the more thorough you wash your hands the lower presence of bacteria there should be.

©eScience Labs, 2018...